Navegando por Palavras-chave "Histoplasmosis"
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- ItemSomente MetadadadosCoccidioidomycosis and Histoplasmosis in Equines: An Overview to Support the Accurate Diagnosis(Elsevier Science Inc, 2016) Brilhante, Raimunda Samia Nogueira; Bittencourt, Paula Vago; Lima, Rita Amanda Chaves; Castelo-Branco, Debora; Oliveira, Jonathas Sales; Pinheiro, Adriana; Cordeiro, Rossana; Camargo, Zoilo Pires [UNIFESP]; Sidrim, Jose Julio Costa; Rocha, Marcos Fabio GadelhaFungal infections of the respiratory tract of horses are not as frequent as those of bacterial and viral origin, often leading to worsening of clinical conditions due to misdiagnosis and incorrect treatment. Coccidioidomycosis and histoplasmosis are systemic mycoses caused by the dimorphic fungi Coccidioides spp. and Histoplasma capsulatum, respectively, which affect humans and a variety of other animals, including equines. These systemic mycoses of chronic and progressive nature can exhibit clinical manifestations similar to other microbial infections. Thus, this article broadly discusses the epidemiology, etiology, virulence, pathogenesis, clinical presentation, treatment, and diagnostic strategies of coccidioidomycosis and histoplasmosis, to support accurate diagnosis. (C) 2016 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosEpidemiology of Invasive Fungal Infections in Patients with Acquired Immunodeficiency Syndrome at a Reference Hospital for Infectious Diseases in Brazil(Springer, 2014-08-01) Oliveira, Renata Buccheri de; Atobe, Jane Harumi; Souza, Simone Aparecida; Castro Lima Santos, Daniel Wagner de [UNIFESP]; Inst Infectol Emilio Ribas; Universidade Federal de São Paulo (UNIFESP)Invasive fungal infections (IFIs) represent one of the main causes of morbimortality in immunocompromised patients. Pneumocystosis, cryptococcosis and histoplasmosis are the most frequently occurring IFIs in patients with acquired immunodeficiency syndrome (AIDS). Fungi, such as Candida spp. and Aspergillus spp., may cause severe diseases during the course of an HIV infection. Following the introduction of highly active anti-retroviral therapy, there has been a marked reduction of opportunistic fungal infections, which today is 20-25 % of the number of infections observed in the mid-1990s. This study is an observational and retrospective study aimed at the characterising IFI incidence and describing the epidemiology, clinical diagnostic and therapeutic features and denouement in HIV/AIDS patients. in HIV/AIDS patients, the IFI incidence is 54.3/1,000 hospitalisation/year, with a lethality of 37.7 %. Cryptococcosis represents the main opportunistic IFI in the population, followed by histoplasmosis. Nosocomial pathogenic yeast infections are caused principally by Candida spp., with a higher candidemia incidence at our institution compared to other Brazilian centres.
- ItemSomente MetadadadosHistoplasmosis in HIV-positive patients in Ceara, Brazil: clinical-laboratory aspects and in vitro antifungal susceptibility of Histoplasma capsulatum isolates(Elsevier B.V., 2012-08-01) Brilhante, Raimunda S. N.; Fechine, Maria A. B.; Mesquita, Jaco R. L.; Cordeiro, Rossana A.; Rocha, Marcos F. G.; Monteiro, Andre J.; Lima, Rita A. C.; Caetano, Erica P.; Pereira, Juliana F.; Castelo-Branco, Debora S. C. M.; Camargo, Zoilo P. [UNIFESP]; Sidrim, Jose J. C.; Univ Fed Ceara; Univ Estadual Ceara; Hosp Sao Jose; Universidade Federal de São Paulo (UNIFESP)This study contains a descriptive analysis of histoplasmosis in AIDS patients between 2006 and 2010 in the state of Ceara, Brazil. Additionally, the in vitro susceptibility of Histoplasma capsulatum isolates obtained during this period was assessed. We report 208 cases of patients with histoplasmosis and AIDS, describing the epidemiological, clinical, laboratory and therapeutic aspects. the in vitro antifungal susceptibility test was carried out by the microdilution method, according to Clinical and Laboratory Standards Institute, with H. capsulatum in the filamentous and yeast phases, against the antifungals amphotericin B, fluconazole, itraconazole, voriconazole and caspofungin. in 38.9% of the cases, histoplasmosis was the first indicator of AIDS and in 85.8% of the patients the CD4 cell count was lower than 100 cells/mm(3). the lactate dehydrogenase levels were high in all the patients evaluated, with impairment of hepatic and renal function and evolution to death in 42.3% of the cases. the in vitro susceptibility profile demonstrated there was no antifungal resistance among the isolates evaluated. There was a significant increase in the number of histoplasmosis cases in HIV-positive patients during the period surveyed in the state of Ceara, northeastern Brazil, but no antifungal resistance among the recovered isolates of H. capsulatum. (C) 2012 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosMolecular identification of Histoplasma capsulatum using rolling circle amplification(Wiley-Blackwell, 2016) Furuie, Jason L.; Sun, Jiufeng; do Nascimento, Mariana M. F.; Gomes, Renata R.; Waculicz-Andrade, Caroline E.; Sessegolo, Gisele C.; Rodrigues, Anderson M. [UNIFESP]; Galvao-Dias, Maria A.; de Camargo, Zoilo P. [UNIFESP]; Queiroz-Telles, Flavio; Najafzadeh, Mohammad J.; de Hoog, Sybren G.; Vicente, Vania A.Histoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, Histoplasma capsulatum, which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of H. capsulatum infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (RCA) detection method which targets ITS (Internal Transcribed Spacer) rDNA of H. capsulatum enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (HcPL2) allowed specific amplification of H. capsulatumDNA while no cross-reactivity was observed with fungi used as negative controls. This method proved to be effective for H. capsulatum specific identification and demonstrated to be faster than the traditional method of microbiological identification.
- ItemSomente MetadadadosMolecular identification of Histoplasma capsulatum using rolling circle amplification(Wiley-Blackwell, 2016) Furuie, Jason L.; Sun, Jiufeng; do Nascimento, Mariana M. F.; Gomes, Renata Rodrigues; Waculicz-Andrade, Caroline E.; Sessegolo, Gisele C.; Rodrigues, Anderson Messias [UNIFESP]; Galvao-Dias, Maria Adelaide; Camargo, Zoilo Pires de [UNIFESP]; Queiroz-Telles, Flavio; Najafzadeh, Mohammad J.; de Hoog, Sybren G.; Vicente, Vania Aparecida; Universidade Federal de São Paulo (UNIFESP)Histoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, Histoplasma capsulatum, which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of H. capsulatum infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (RCA) detection method which targets ITS (Internal Transcribed Spacer) rDNA of H. capsulatum enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (HcPL2) allowed specific amplification of H. capsulatumDNA while no cross-reactivity was observed with fungi used as negative controls. This method proved to be effective for H. capsulatum specific identification and demonstrated to be faster than the traditional method of microbiological identification.
- ItemAcesso aberto (Open Access)Ocular histoplasmosis-like syndrome: a report from a nonendemic area(Conselho Brasileiro de Oftalmologia, 2007-08-01) Amaro, Miguel Hage [UNIFESP]; Muccioli, Cristina [UNIFESP]; Abreu, Mariza Toledo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To report some cases of ocular histoplasmosis-like syndrome from a nonendemica area. DESIGN: Observational case series. METHODS: This is a prospective study of 16 eyes from 8 immunocompetent patients evaluated between January 2001 to September 2005. Six patients were female and 2 male aged between 20 to 44 years, average 28 years. All patients presented clinical features that resembled ocular histoplasmosis. All patients had a negative antibody test for histoplasmosis and negative medical and laboratory evaluation of toxoplasmosis, syphilis, and tuberculosis. All patients were submitted to a complete ocular examination including fluorescein angiography. One patient was submitted to indocyanine green angiography. RESULTS: Five patients presented the classical triad of clinical features that include peripapillary scarring, histo spots, choroidal neovascularization, one patient presentd enlargement of atrophic chorioretina scar, one patient presented multiple retinal pigment epithelial detachment, one, neovascularized retinal pigment epithelial detachment. Another patient presented only histo spots. CONCLUSION: These findings of ocular histoplasmosis-like syndrome in patients with negative antibody serum test from a nonendemica area suggest that other agents could cause these similar fundus findings of ocular histoplasmosis.
- ItemSomente MetadadadosRYP1 gene as a target for molecular diagnosis of histoplasmosis(Elsevier Science Bv, 2016) Nogueira Brilhante, Raimunda Samia; de Melo Guedes, Glaucia Morgana; Riello, Giovanna Barbosa; Ribeiro, Joyce Fonteles; Alencar, Lucas Pereira; Bandeira, Silviane Praciano; Collares Maia Castelo-Branco, Debora Souza; Oliveira, Jonathas Sales; Maia Freire, Janaina Maria; Lima de Mesquita, Jaco Ricarte; Camargo, Zoilo Pires de [UNIFESP]; Cordeiro, Rossana de Aguiar; Gadelha Rocha, Marcos Fabio; Costa Sidrim, Jose JulioThis study analyzed the RYP1 gene as a target for the molecular diagnosis of histoplasmosis. This assay detected fungal DNA in 13/13 blood samples from HIV/AIDS-patients with histoplasmosis. Therefore, the detection of RYP1 gene in whole blood sample is a quick and sensitive test to diagnose histoplasmosis. (C) 2016 Elsevier B.V. All rights reserved.