The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline

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dc.citation.issue11
dc.citation.volume32
dc.contributor.authorRibeiro de Oliveira, Teresinha Regina
dc.contributor.authorde Oliveira, Geraldo Ferreira
dc.contributor.authorSimoes, Ricardo Santos
dc.contributor.authorFeitosa, Suellen Maurim [UNIFESP]
dc.contributor.authorTikazawa, Eduardo Hiroshi
dc.contributor.authorMonteiro, Hugo Pequeno [UNIFESP]
dc.contributor.authorFagundes, Djalma Jose [UNIFESP]
dc.contributor.authorTaha, Murched Omar [UNIFESP]
dc.coverageSao Paulo
dc.date.accessioned2020-09-01T13:21:21Z
dc.date.available2020-09-01T13:21:21Z
dc.date.issued2017
dc.description.abstractPurpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+ R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes). Results: The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P< 0.05) compared to IR+ SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P> 0.05). The NOS-2 expression (qRTPCR) in the IRPTX group (P< 0.05) was higher than the values for the IPC+ IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P< 0.05), the IR-SS (P< 0.05) and the IR-PTX (P< 0.05) groups. Conclusions: The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect.en
dc.description.affiliationUFGD, Sch Hlth Sci, Dourados, MS, Brazil
dc.description.affiliationUniv Sao Paulo, UNIFESP, Dept Morfol & Genet, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Gynecol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Sch Med, Dept Publ Hlth, Campinas, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Surg, Div Surg Tech & Expt Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Sao Paulo, UNIFESP, Dept Morfol & Genet, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Gynecol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Surg, Div Surg Tech & Expt Surg, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMinisterio da Educacao do Brasil, UFGD
dc.format.extent935-948
dc.identifierhttp://dx.doi.org/10.1590/s0102-865020170110000005
dc.identifier.citationActa Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 935-948, 2017.
dc.identifier.doi10.1590/s0102-865020170110000005
dc.identifier.fileS0102-86502017001100935.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502017001100935
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58221
dc.identifier.wosWOS:000417846700005
dc.language.isoeng
dc.publisherActa Cirurgica Brasileira
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIschemic Preconditioningen
dc.subjectPentoxifyllineen
dc.subjectIschemiaen
dc.subjectReperfusion Injuryen
dc.subjectNitric Oxide Synthaseen
dc.subjectApoptosisen
dc.subjectRatsen
dc.titleThe expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifyllineen
dc.typeinfo:eu-repo/semantics/article
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