Repeated electroconvulsive shock induces changes in high-affinity [H-3]-ouabain binding to rat striatal membranes
| dc.contributor.author | Bignotto, Magda [UNIFESP] | |
| dc.contributor.author | Venditti, Marco Antonio Campana [UNIFESP] | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T12:41:03Z | |
| dc.date.available | 2016-01-24T12:41:03Z | |
| dc.date.issued | 2006-04-01 | |
| dc.description.abstract | Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium-potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a long time. Sodium-potassium/ATPase has an extracellular regulatory binding site to which cardiotonic glycosides, such as ouabain, bind to, thus regulating the activity of the enzyme. Endogenous ouabain-like substances exist in the brain and their actions on the sodium-potassium/ATPase resemble ouabain biological properties. the aim of this work was to determine if electroconvulsive shock (ECS) would induce changes in the high-affinity binding of ouabain to the sodium-potassium/ATPase from rat brain regions. Adult, male Wistar rats received one (ECSx1 group) or seven electroshocks (ECSx7 group) delivered daily through ear-clips electrodes. Control rats received the same manipulations; however, no current was delivered through the electrodes (SHAMx1 and SHAMx7 groups). All groups were sacrificed 24 h after the last ECS session. the B-max and K-D of high-affinity [3H]-ouabain binding were determined in crude membrane preparations from the striatum, hippocampus and brainstem. the results obtained showed a statistically significant increase in the affinity of [H-3]-ouabain (lower K (D)) to striatal membranes in those rats receiving seven ECS. in the striatum there was no change in the K (D) after one ECS; as well as there was no change in the B-max after a single or seven ECS. High-affinity [H-3]-ouabain binding to hippocampus and brainstem did not reveal any significant differences either in K (D) or B-max after one or seven ECS. the increased affinity of ouabain to the striatal sodium-potassium/ATPase induced by repeated ECS suggests an increased interaction in vivo of the endogenous ouabain-like substances with the enzyme and the involvement of the extracellular regulatory allosteric ouabain binding site in the striatal sodium-potassium/ATPase in the effects of electroconvulsive shock. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Dept Psicobiol, BR-04023900 São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Dept Psicobiol, BR-04023900 São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.format.extent | 515-521 | |
| dc.identifier | http://dx.doi.org/10.1007/s11064-006-9046-6 | |
| dc.identifier.citation | Neurochemical Research. New York: Springer/plenum Publishers, v. 31, n. 4, p. 515-521, 2006. | |
| dc.identifier.doi | 10.1007/s11064-006-9046-6 | |
| dc.identifier.issn | 0364-3190 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/28808 | |
| dc.identifier.wos | WOS:000238138800009 | |
| dc.language.iso | eng | |
| dc.publisher | Springer | |
| dc.relation.ispartof | Neurochemical Research | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
| dc.subject | electroconvulsive shock | en |
| dc.subject | [H-3]-ouabain | en |
| dc.subject | sodium-potassium/ATPase | en |
| dc.subject | striatum | en |
| dc.subject | hippocampus | en |
| dc.subject | brainstem | en |
| dc.title | Repeated electroconvulsive shock induces changes in high-affinity [H-3]-ouabain binding to rat striatal membranes | en |
| dc.type | info:eu-repo/semantics/article |