PPG - Tecnologias e Atenção à Saúde

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Navegando PPG - Tecnologias e Atenção à Saúde por Orientador(es) "Andrade, Luiz Eduardo Coelho Andrade [UNIFESP]"

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    Caracterização do teste de imunofluorescência indireta em células hep-2 de anticorpos antinúcleo em enfermos não-autoimunes
    (Universidade Federal de São Paulo (UNIFESP), 2015-08-19) Agustinelli, Renan de Almeida [UNIFESP]; Andrade, Luiz Eduardo Coelho Andrade [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objectives: to assess the frequency of antinuclear antibodies (ANA)-HEp-2 test results, the distribution of various immunofluorescence patterns and reaction titers in patients with various non-autoimmune diseases and compare these parameters to those presented by patients with systemic lupus erythematosus (SLE) and healthy individuals. Patients and Methods: cross-sectional observational study in which 588 patients were evaluated and separated into 4 subgroups (152 patients with multiple co-morbidities, 95 patients with neoplasms, 148 with infectious diseases and 163 with psychiatric diseases), 150 patients with SLE and 918 healthy individuals. We considered the ANA-HEp-2 positive in titers higher than 1:80 using commercial slides evaluated by 2 independent examiners. Results: positive ANA-HEp-2 was observed in 102 (18,3%) patients with non-autoimmune disease, in 146 (96%) of patients with SLE and 118 (12,9%) of healthy individuals. No significant differences were found in titers or patterns between the subgroups of patients with non-autoimmune disease. The titers for ANA-HEp-2 in SLE were higher than in patients with non-autoimmune disease, and, when comparing the titers of the non-autoimmune patients with healthy individuals we found titers 1:160 (p < 0,001) and 1:320 (p =0,001) more frequently in the first group and 1:80 in the second group (p <0,001). The nuclear homogenous and nuclear coarse speckled patterns were more frequent in SLE patients (p < 0,001). In the comparison between patients with non-autoimmune disease and healthy individuals we found higher incidences of nuclear dense fine speckled patterns in the second group (p = 0,004). The most common pattern in all groups was the nuclear fine speckled, presenting lower titers in healthy individuals and in patients with non-autoimmune disease (p < 0,001). When comparing the titers of nuclear fine speckled patterns between patients with non-autoimmune disease and in healthy ones we found the titer 1:160 more frequently among the first group (p = 0,003) and 1:80 in the second group (p < 0,001). Conclusion: our results suggest that the pattern and titer of ANA-HEp-2 are useful parameters to differentiate, not only ANA-HEp-2 results in healthy individuals and in SLE patients, but also to distinguish patients with non-autoimmune disease.