PPG - Patologia

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Navegando PPG - Patologia por Autor "Bernardo, Marcia Cristina [UNIFESP]"

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    Perfil Imunohistoquímico De Painel Das Citoqueratinas (5,7,14,18,20), Em Uma Série Retrospectiva De Fibroadenoma De Mama
    (Universidade Federal de São Paulo (UNIFESP), 2017-02-15) Bernardo, Marcia Cristina [UNIFESP]; Waitzberg, Angela Flavia Logullo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objetive: Cytokeratin expression profile And Progenitor Cell Contingent In Fibroadenomas. An Imunohistochemical Assessment Of Proliferation And Cytokeratins 5,7,14, 18 And 20. Study Design: Fibroadenomas (FA) are the most common benign tumor in breast. Described as a biphasic stromal and epithelial lesion, it is usually stable and clinically well managed. However, few cases present as multiple or even recur after excision. Although distinct cytokeratin (CK) distribution among ductal cells is well described in normal breast tissue and ductal carcinomas, how different in terms of differentiation and distribution of progenitor cells are in FA is scarcely known. We therefore verified if Epithelial- mesenchymal relationship could influence the distinct CK expression in ductal cells in FA and if their contingent of CK5 and CK14 positive cells, as a progenitor cell profile, is comparable to the normal breast. A retrospective series of FA diagnosed and surgically treated at Pérola Byington Hospital, São Paulo, Brazil between 2005 and 2006 were evaluated with a 5 year follow up. After paraffin blocks, slides and clinical data available were reviewed, 104 cases were included and proper areas were identified and included in a tissue micro array containing ductal epithelial samples. CK expression was assessed by counting the positive and negative number of ductal cells contained in terminal ductal acinar units and also registering the basal or luminal position of positive cells. Ki-67 expression score evaluated the contingent of positive cells in 100 ductal cells. All data were statistically evaluated by parametric T- student and non-parametric Fisher tests. Mac Nemmar and Mann –Whitney tests verified the differences among CK expressions. FA express CK 5, 7 14,18 and not CK 20, distribution and percentage of positive cells are comparable to the normal breast profile. Progenitor cells contingent, when considering CK5 and Ck14 positive cells, is equal or higher than in normal breast tissue. Proliferation score is maintained comparable to the normal tissue in FA, and is correlated to CK5 expression, but not to CK14 or 18 or tumor size. CK 18 was associated to early age (p=0,0368).14 cases recurred in our series. Recurrence was not associated to clinical variables (age, tumor size) Ki-67 score or CKs expression.