PPG - Bioprodutos e Bioprocessos

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https://hdl.handle.net/11600/61205

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Navegando PPG - Bioprodutos e Bioprocessos por Autor "Costa, Marcia Busanello [UNIFESP]"

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    Avaliação dos efeitos do fator de crescimento epidérmico (EGF) associado a terapia de fotobiomodulação a LED no reparo tecidual em modelo experimental de feridas cutâneas em ratos
    (Universidade Federal de São Paulo (UNIFESP), 2020-08-28) Costa, Marcia Busanello [UNIFESP]; Garcia, Livia Assis [UNIFESP]; Universidade Federal de São Paulo
    Epidermal Growth Factor (EGF) and LED light therapy have been highlighted as promissory treatments for skin repair, however their mechanisms of their association have not yet been clarified. The aim of this study was to compare the effects of combined treatment with Epidermal Growth factor (EGF) and red light-emitting diode (LED) on the wound healing processes in rats. Forty male Wistar rats were randomly divided into 4 groups: control group (CG) wounds without treatment; wounds submitted to EGF treatment (EGF); wounds submitted to LED treatment (LED); wounds submitted to EGF associated with LED treatments (EGF/PBM). Treatments were starting immediately after the surgery and they were performed 8 consecutive days. Moreover, LED was performed before EGF treatment at a single point in the center of the wound. Morphological characteristics and the immunoexpression of wound healing markers were evaluated. The results demonstrated that EGF/LED group presented a higher wound healing index. Additionally, all experimental groups presented similar findings in the histological evaluation, the degree of inflammation and area of dermis-like tissue. However, for EGF treated animals (with or without LED) neoepithelial length was higher. Furthermore, all the treated groups decreased COX-2 and increased VEGF imunoexpression and only EGF/LED group enhanced the TGF-β protein expression compared to CG. This study showed that EGF and LED modulate inflammatory process and increase the vascularity. However, the associated treatments showed a more evident positive effect for increasing TGFβ expression and may be promising resources in the clinical treatment of cutaneous wounds.