Navegando por Palavras-chave "Abdominal Hernial/Surgery"

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    Estudo comparativo da deposição do colágeno tipo 1 e tipo 3 nos períodos precoce e tardio entre as telas de polipropileno de alta densidade, polipropileno de baixa densidade, polipropileno revestido por celulose, e politetrafluoretileno expandido: modelo experimental em ratos
    (Universidade Federal de São Paulo (UNIFESP), 2020-10-29) Maeda, Carlos Toshinori [UNIFESP]; Linhares, Marcelo Moura [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Abdominal wall hérnia is the most prevalent disease of surgical treatment worldwide. Nowadays, the prostheses reinforcement is the standard treatment, However the ideal mesh for the different scenarios is still a controversial topic in the literature. It is known that the final surgery outcome depends directly of the healing tissues quality induced by the meshes and that could be basically assessed quantifying 1 collagen type 1, collagen type 3, as well as the proportion between these two proteins. Objectives: Evaluate in the Early and Late Periods, the deposition of collagen 1, collagen 3 and the proportion of collagen 1 over collagen 3, between four types of meshes. Methods: Eighty Wistar rats were submitted to a ventral hernia model surgery with mesh placement. They were primarily divided into four large groups: Group PPA (high density polypropylene mesh); Group PPB (low density polypropylene mesh); Group PPCE (low density polypropylene encapsulated with polydioxanone and coated with oxidized cellulose); and Group PTFE (expanded polytetrafluoroethylene mesh). These groups were then subdivided into Early Period (submitted to euthanasia on the 7th postoperative day) and Late Period (submitted to euthanasia on the 28th postoperative day). For immunohistochemical analysis, polyclonal anti-collagen 1 and anti-collagen 3 antibodies were used and evaluated using a semi-quantitative scale. To analyze the proportion of collagens, picrosirius staining method with differentiation by the birefringence method was used, and analysis was made with the “Image J” software. The results were compared between meshes and between periods. Results: In the Early Period, Group PPCE had a higher collagen 1 mean than group B (p=0.003) and Group PTFE (p=0.001), the was no difference between groups in collagen 3 means. In the Late Period the Group PPB present higher mean than Group PPA (p = 0.005) and Group PTFE (p=0.002), and again there was no difference comparing collagen 3. When comparing Early and Late Periods, Groups PPA (p=0.022) and PPB (p<0.001) showed an increase of collagen 1 quantification, while Group PPCE (p= 0.01) showed an increase collagen 3 mean. In the analysis of the proportions of type 1 collagen over type collagen 3 using the picrosirius method, there was no statistical differences in the Early and Late Periods; When comparing Early and Late Periods, Group PPA (p=0.001); and PPB (p=0.018) showed an increase mean of this proportion. Conclusions: In the Early xvii Period, deposition of type 1 collagen was higher in cellulose-coated polypropylene mesh than low-density polypropylene and expanded polytetrafluoroethylene mesh, while in the Late Period, low-density polypropylene mesh showed greater collagen 1 deposition when compared to high density polypropylene and expanded polytetrafluoroethylene mesh. There was no difference in collagen 3 deposition of between the prostheses, in both Periods. The high and low density polypropylene meshes showed an increase collagen 1 deposition, while the polypropylene mesh coated with cellulose showed an collagen 3 increased between the Early and Late Periods. The ratio of collagens 1 over 3 was similar between prostheses in the studied periods. However, high and low density polypropylene meshes showed an increase ratio in the Late Period.