Navegando por Palavras-chave "Amicacina"
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- ItemAcesso aberto (Open Access)Caracterização molecular de amostras de Pseudomonas aeruginosa resistentes a Amicacina e Meropenem(Universidade Federal de São Paulo (UNIFESP), 2019-02-20) Santos, Paulo Henrique Dantas Dos [UNIFESP]; Pignatari, Antonio Carlos Campos [UNIFESP]; http://lattes.cnpq.br/9461346610553865; http://lattes.cnpq.br/7071042329034394; Universidade Federal de São Paulo (UNIFESP)Introduction: Pseudomonas aeruginosa infections in the hospital environment present high morbidity and mortality, particularly in immunocompromised patients, with high rates of multiresistance to antimicrobials including carbapenens and aminoglycosides. This study retrospectively analyzed isolates of P. aeruginosa resistant to amikacin and meropenem from nosocomial infections of the bloodstream and respiratory tract diagnosed in the participating medical centers of SCOPE Brazil and Hospital São Paulo. Methods: The sensitivity profile to amikacin and meropenem of 96 P. aeruginosa samples, identified by MALDI-TOF, was determined by dilution in agar. The presence of genes encoding enzyme resistance to meropenem and amikacin was evaluated by real-time PCR (qPCR) and the clonality profile by Pulsed Field Gel Electrophoresis (PFGE). Four samples with different minimum inhibitory concentrations (MIC) for amikacin were submitted to new generation sequencing (SNG). Results: Resistance to both antibiotics, amikacin and meropenem, was observed in 42 (43.75%) with very high MICs for amikacin (> 4,096 μg / ml). The blaSPM, rmtD, blaGES resistance genes were detected by the qPCR methodology. An association of samples with higher MICs for amikacin was observed when the detection of genes coding for the metalloenzyme blaSPM and the methylase rmtD was observed. The analysis of the clonality profile of the isolates resistant to amikacin by PFGE revealed 10 profiles, and the majority of isolates resistant to amikacin and meropenem are found in the same profile. The SNG analyzed by the online platforms Center for Genomic Epidemiology and MLST-Pasteur Database enabled the detection of resistance genes for β-lactams, aminoglycosides and fluoroquinolones, as well as ST of three isolates, especially ST 277 for international dissemination.
- ItemSomente MetadadadosEfeito da oxigenação hiperbárica em lesão ototóxica produzida pela amicacina em cobaias(Universidade Federal de São Paulo (UNIFESP), 2012) Amora, Luciana de Albuquerque Salviano [UNIFESP]; Fagundes, Djalma José [UNIFESP]Objetivo: estudar os efeitos da oxigenacao hiperbarica (OHB) em lesao ototoxica produzida pela Amicacina (AM) administrada pelas vias transtimpanica (TT) ou subcutanea (SC). Metodos: Foram estudados os aspectos funcionais de 24 cobaias albinas, atraves das emissoes otoacusticas produto de distorcao (EOADP) e do potencial evocado auditivo de tronco encefalico (PEATE), previa e posteriormente a aplicacao da Amicacina (600 mg/Kg/dia) e das sessoes de oxigenacao hiperbarica, 2 ATA, 60 minutos, tres sessoes. Os aspectos morfologicos foram avaliados atraves da microscopia eletronica de varredura (MEV). Foram constituidos dois grupos; Grupo com perfuracao timpanica (12 animais) subdividido em quatro subgrupos: OHB I + SS (3 animais, solucao salina + OHB), AM TT (3 animais, AM 8 dias), AM TT + R (3 animais, AM 8 dias + 7 dias de repouso) e AM TT + OHB (3 animais, AM 8 dias + OHB). Grupo sem perfuracao timpanica (12 animais) subdividido em quatro subgrupos: OHB II + SS (3 animais, solucao salina + OHB), AM SC (3 animais, AM 8 dias), AM SC + R (3 animais, AM 8 dias + repouso 7 dias) e AM SC + OHB (3 animais, AM 8 dias + OHB). Resultados: Os animais submetidos apenas a solucao salina e OHB demonstraram preservacao tanto dos aspectos funcionais quanto dos morfologicos durante todo experimento. Os animais submetidos a AM tanto pela via transtimpanica quanto pela via subcutanea apresentaram lesoes significativas das celulas ciliadas da coclea, bem como alteracoes funcionais ao final do experimento. Os animais submetidos a AM e a algum tipo de procedimento, seja OHB ou repouso tambem apresentaram alteracoes significativas tantos dos aspectos funcionais quanto dos morfologicos, nao apresentando diferencas estatisticamente significantes com os animais nao tratados. Conclusao: A OHB nao promoveu alteracoes significativas na morfologia das celulas ciliadas da coclea e nos limiares eletrofisiologicos das cobaias submetidas ao uso de AM nas duas formas de administracao propostas neste estudo. O estado funcional das celulas ciliadas externas da coclea de cobaias submetidas ao uso de AM pela via subcutanea nao apresentou ganho significativo apos a OHB
- ItemAcesso aberto (Open Access)Efeito de fármacos ototóxicos na audição de recém-nascidos de alto risco(Sociedade Brasileira de Fonoaudiologia, 2010-01-01) Camara, Marília Fontenele e Silva; Azevedo, Marisa Frasson de [UNIFESP]; Lima, José Wellington de Oliveira; Sartorato, Edi Lúcia; Universidade de Fortaleza; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual do Ceará; Universidade Estadual de Campinas (UNICAMP)PURPOSE: To calculate the incidence of sensorineural hearing loss (SNHL), to verify if there is a causal association between the use of ototoxic drugs and SNHL, and to establish the frequency of genetic mutations related to SNHL in high risk newborns. METHODS: The study was a retrospective and prospective cohort research with 250 children. Data was gathered from subjects' charts and with their caregivers. Moreover, subjects were submitted to auditory evaluation with distortion product otoacoustic emissions, timpanometry, visual reinforcement audiometry, auditory brainstem response and transient otoacoustic emissions. The study of the genetic mutation 35delG, and the mitochondrial mutations A1555G and A7445G was essential to evaluate the possibility that SNHL had a non-syndromic genetic origin. The association between the medicine use and the occurrence of hearing loss had been analyzed. RESULTS: The incidence of SNHL in high risk newborns was 11.6%, and causal associations between SNHL and the drugs administered were: amikacin and cefotaxime (OR=5.35), cefotaxime and furosemide (OR=7.02), ceftazidime and vancomycin (OR=9.12). The frequencies of the mutation 35deIG and mitochondrial mutations A1555G and A7445G were, respectively, 0.8% and 0%. CONCLUSION: The incidence of SNHL in high risk newborns was high, showing an important causal relation with the use of ototoxic drugs and a small relation with genetic mutations.