Navegando por Palavras-chave "Anti-inflammatory activity"

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    The anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in mice
    (Springer, 2013-08-01) Morais-Zani, Karen de; Barbosa Nunes, Fernanda Peixoto; Silva, Jacilene Barbosa da; Ferreira, Marcio Jose; Grego, Kathleen Fernandes; Lopes-Ferreira, Monica; Tanaka, Aparecida Sadae [UNIFESP]; Tanaka-Azevedo, Anita Mitico; Inst Butantan; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Antithrombin is known as the most important natural coagulation inhibitor and has been shown to have anti-inflammatory properties. the present study aimed to investigate the effects of Bothrops jararaca antithrombin on acute inflammation induced by carrageenan in mice.We evaluated the anti-inflammatory activity of antithrombin on models of paw edema formation, cell migration and leukocyte-endothelium interaction in mice (Swiss; n = 5). Acute inflammation was induced by the administration of carrageenan (15 mg kg(-1)).Treatment with B. jararaca antithrombin (1 mg kg(-1)) 1 h before or after carrageenan administration significantly inhibited paw edema formation, reduced cell influx to the peritoneal cavity due to reduction in the migration of polymorphonuclear cells, and attenuated leukocyte rolling in the microcirculation of the cremaster muscle.The effects of antithrombin on vascular and cellular events of inflammation were completely abolished by treatment with the cyclo-oxygenase inhibitor indomethacin (4 mg kg(-1)), suggesting the involvement of prostacyclin in the mechanism of inflammation inhibition by B. jararaca antithrombin.This work showed for the first time the anti-inflammatory properties of B. jararaca antithrombin on vascular and cellular events of inflammation. These findings suggest that antithrombin is effective in preventing paw edema formation, cell migration and leukocyte rolling induced by carrageenan in mice.
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    Anti-inflammatory properties of a heparin-like glycosaminoglycan with reduced anti-coagulant activity isolated from a marine shrimp
    (Elsevier B.V., 2008-11-01) Brito, Adriana S.; Arimateia, Dayse S.; Souza, Lucilla R.; Lima, Marcelo A.; Santos, Vanessa O.; Medeiros, Valquiria P. [UNIFESP]; Ferreira, Paula A.; Silva, Rodrigo A.; Ferreira, Carmen V.; Justo, Giselle Z. [UNIFESP]; Leite, Edda L.; Andrade, Giulianna P. V.; Oliveira, Fernanda W.; Nader, Helena B. [UNIFESP]; Chavante, Suely F.; Univ Fed Rio Grande do Norte; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)
    The anti-inflammatory properties of a heparin-like compound from the shrimp Litopenaeus vannamei are related. Besides reducing significantly (p < 0.001) the influx of inflammatory cells to injury site in a model of acute inflammation, shrimp heparin-like compound was able to reduce the matrix metalloproteinase (MMPs) activity in the peritoneal lavage of inflamed animals. Moreover, this compound also reduced almost 90% the activity of MMP-9 secreted by human activated leukocytes. Negligible anti-coagulant activities in aPPT assay and a poor bleeding potential make this compound a better alternative than mammalian heparin as a possible anti-inflammatory drug. (C) 2008 Elsevier B.V. All rights reserved.
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    Anti-secretory, anti-inflammatory and anti-Helicobacter pylori activities of several fractions isolated from Piper carpunya Ruiz & Pav.
    (Elsevier B.V., 2010-04-21) Quilez, A.; Berenguer, B.; Gilardoni, G.; Souccar, C. [UNIFESP]; Mendonca, S. de; Oliveira, L. F. S.; Martin-Calero, M. J.; Vidari, G.; Univ Seville; Univ Pavia; Universidade Federal de São Paulo (UNIFESP); Univ Bandeirante São Paulo
    Ethnopharmacological relevance: the leaves of Piper carpunya Ruiz & Pay. (syn Piper lenticellosum C.D.C.) (Piperaceae), are widely used in folk medicine in tropical and subtropical countries of South America as an anti-inflammatory, anti-ulcer, anti-diarrheal and anti-parasitical remedy as well as an ailment for skin irritations.Aims of the study: To study the anti-inflammatory, anti-secretory and anti-Helicobacter pylori activities of different fractions isolated from an ethanolic extract of the leaves of Piper carpunya, in order to provide evidence for the use of this plant as an anti-ulcer remedy. Moreover, to isolate the main compounds of the extract and relate their biological activity to the experimental results obtained with the fractions.Materials and methods: Sixteen fractions were obtained from the ethanolic extract (FI-XVI) and 16 pure compounds were isolated and identified from these fractions. We studied the effects of the fractions (0.1-400 mu g/mL) on the release of myeloperoxidase (MPO) enzyme from rat peritoneal leukocytes, on rabbit gastric microsomal H(+), K(+)-ATPase activity and anti-Helicobacter pylori anti-microbial activity using the microdilution method (MM). the main compounds contained in the fractions were isolated and identified by (1)H- and (13)C NMR spectra analysis and comparison with the literature data.Results: Eight fractions showed inhibition of MPO enzyme (FI-IV, X, XII, XIV and XV). the highest inhibition was observed with F XIV (50 mu g/mL, 60.9%, p < 0.001). F X and XII were the most active ones, inhibiting the gastric H(+), K(+)-ATPase activity with IC(50) values equal to 22.3 mu g/mL and 28.1 mu g/mL, respectively. All fractions, except F XV, presented detectable anti-Helicobacter pylori activity, with a diameter of inhibition zones ranging from 11 mm up to 50 mm. the best anti-Helicobacter pylori activity was obtained with F III and V. Both fractions killed Helicobacter pylori with lowest concentration values, about 6.25 mu g/mL. Sixteen pure compounds were isolated, five of them are flavonoids that possess strong anti-oxidant and free radical scavenging activity, e.g. vitexin, isovitexin, and rhamnopyranosylvitexin. Terpenoids like sitosterol, stigmasterol and phytol, which have shown gastroprotective activity, and dihydrochalcones, like asebogenin, with anti-bacterial activity, were also isolated. Furthermore, the rare neolignan 1, that is a DNA polymerase 3 lyase inhibitor, and (6S, 9S)-roseoside, that shows strong anti-bacterial activity, were isolated, for the first time, from the genus Piper.Conclusions: We suggest that the flavonoids isolated from F I and II (vitexin, isovitexin, rhamnopyranosylvitexin and isoembigenin) contribute to the anti-MPO activity, as well as to their anti-Helicobacter pylori activity. These flavonoids may also be responsible for the important inhibition of H(+), K(+)-APase activity. Also the phytosterols and phytol obtained from F XIV and XV could be involved in these gastroprotective activities. These results encourage us to continue phytochemical studies on these fractions in order to obtain full scientific validation for this species. (C) 2010 Elsevier Ireland Ltd. All rights reserved.