Navegando por Palavras-chave "Anticoagulant activity"
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- ItemSomente MetadadadosAnti-inflammatory properties of a heparin-like glycosaminoglycan with reduced anti-coagulant activity isolated from a marine shrimp(Elsevier B.V., 2008-11-01) Brito, Adriana S.; Arimateia, Dayse S.; Souza, Lucilla R.; Lima, Marcelo A.; Santos, Vanessa O.; Medeiros, Valquiria P. [UNIFESP]; Ferreira, Paula A.; Silva, Rodrigo A.; Ferreira, Carmen V.; Justo, Giselle Z. [UNIFESP]; Leite, Edda L.; Andrade, Giulianna P. V.; Oliveira, Fernanda W.; Nader, Helena B. [UNIFESP]; Chavante, Suely F.; Univ Fed Rio Grande do Norte; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)The anti-inflammatory properties of a heparin-like compound from the shrimp Litopenaeus vannamei are related. Besides reducing significantly (p < 0.001) the influx of inflammatory cells to injury site in a model of acute inflammation, shrimp heparin-like compound was able to reduce the matrix metalloproteinase (MMPs) activity in the peritoneal lavage of inflamed animals. Moreover, this compound also reduced almost 90% the activity of MMP-9 secreted by human activated leukocytes. Negligible anti-coagulant activities in aPPT assay and a poor bleeding potential make this compound a better alternative than mammalian heparin as a possible anti-inflammatory drug. (C) 2008 Elsevier B.V. All rights reserved.
- ItemAcesso aberto (Open Access)A heparin-like compound isolated from a marine crab rich in glucuronic acid 2-O-sulfate presents low anticoagulant activity(Elsevier B.V., 2013-04-15) Andrade, Giuliana Paiva Viana de; Lima, Marcelo Andrade de [UNIFESP]; Souza Junior, Airton Araujo de [UNIFESP]; Fareed, Jawed; Hoppensteadt, Debra A.; Santos, Elizeu Antunes dos; Chavante, Suely Ferreira; Oliveira, Fernanda Wanderley de; Rocha, Hugo Alexandre de Oliveira; Nader, Helena Bonciani [UNIFESP]; Univ Fed Rio Grande do Norte; Universidade Federal de São Paulo (UNIFESP); Univ Liverpool; Inst Fed Rio Grande Norte; Loyola Med SchA natural heparin-like compound isolated from the crab Goniopsis cruentata was structurally characterized and its anticoagulant and hemorrhagic activities were determined. Enzymatic and nuclear magnetic resonance analysis revealed that its structure is rich in disulfated disaccharides, possessing significant amounts of 2-O-sulfated-beta-D-glucuronic acid units. Furthermore, low amounts of trisulfated disaccharide units containing 2-O-sulfated-alpha-L-iduronic acid were detected, when compared to mammalian heparin. in addition, this heparin-like structure showed negligible in vitro anticoagulant activity and low bleeding potency, facts that make it a suitable candidate for the development of structure-driven, heparin based therapeutic agents with fewer undesirable effects. (C) 2013 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosA heparin-like glycosaminoglycan from shrimp containing high levels of 3-O-sulfated D-glucosamine groups in an unusual trisaccharide sequence(Elsevier B.V., 2014-05-22) Chavante, Suely F.; Brito, Adriana S.; Lima, Marcelo [UNIFESP]; Yates, Edwin [UNIFESP]; Nader, Helena [UNIFESP]; Guerrini, Marco; Torri, Giangiacomo; Bisio, Antonella; Univ Fed Rio Grande do Norte; Universidade Federal de São Paulo (UNIFESP); Univ Liverpool; Ist Ric Chim & Biochim G RonzoniThe detailed characterization of a novel heparin-like glycosaminoglycan purified from the viscera (heads) of the shrimp Litopenaeus vannamei is reported. Structural analysis performed by mono-and two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed it to be rich in both glucuronic acid and N, 6-sulfated glucosamine residues. the key peculiarities were its high 3-O-sulfated glucosamine content compared to mammalian heparins; a residue which is usually associated with the antithrombin (AT) binding site, and the location of these residues within 2-O-sulfated iduronate and glucuronate-containing sequences (I(2S-)A*-G), a situation not found in mammalian heparin. It also exhibited higher molecular weight (similar to 36 kDa) than conventional heparin (similar to 16 kDa) but, negligible anticoagulant activity (similar to 5 IU/mg compared to heparin similar to 190 IU/mg) and stabilization of AT, which has been linked directly to anticoagulation activity. A high affinity fraction, eluting at a similar salt concentration (0.75-1.5 M NaCl) from an antithrombin affinity column, to the high affinity fraction of heparin, also showed only weak thermal stabilization of AT (+ similar to 2 degrees C). These structural peculiarities may help elucidate more clearly the relationship between structure and function of sulfated polysaccharides, and provide useful model compounds with which to better understand interactions of biological significance. (C) 2014 Published by Elsevier B.V.
- ItemAcesso aberto (Open Access)Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora(Associação Brasileira de Divulgação Científica, 2005-04-01) Silva, T.m.a.; Alves, L.g. [UNIFESP]; Queiroz, K.c.s. de; Santos, M.g.l.; Marques, C.t.; Chavante, Suely Ferreira [UNIFESP]; Rocha, Hugo Alexandre Oliveira [UNIFESP]; Leite, E.l.; Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia; Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais; Universidade Federal de São Paulo (UNIFESP)The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.