Navegando por Palavras-chave "Antitumor Drug Selection Tests"

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    Desenho De Novos Peptídeos Catiônicos A Partir Do Sensor De Voltagem De Canais Iônicos
    (Universidade Federal de São Paulo (UNIFESP), 2018-05-24) Lima, Estevao Carlos [UNIFESP]; Miranda Filho, Manoel De Arcisio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: Design Of Novel Cationic Peptides Through The Analysis Of Ion Channel Voltage Sensor Region And Analysis Of Their Bioactivity In Respect Of Anticancer And Antibacterial Activities. Methods: The Amino Acid Sequence Of 104 Ion Channels Of Nav, Cav And Kv Families Were Multialigned So That The S4 Region Could Be Identified. Based On The Distribution Frequency Of This Residues, Three Peptides Were Designed And Their Secondary Structure Was Evaluated By Circular Dichroism Technique With The Use Of Large Unilamelar Vesicles (Luvs). This Luvs Had The Biomimetic Characteristics Of: Mammal Cells [Popc:Col (70:30 Mol%)]; Tumoral Cell [Popc:Col:Pops(50:30:20)]; And Bacterial Strains [Popc:Popg (50:50)]. Anticancer Activity Was Measured By The Mtt Reduction Assay Using Tumoral (Hela; Du 145; Mcf 7) And Non Tumoral (Nih 3t3) Cell Lines Incubated With The Peptides Over A Concentration Range. The Antibacterial Activity Was Measured By An Optical Density Analysis Using Gram Negative And Gram Positive Strains. The