Navegando por Palavras-chave "Bacterial eye infections"
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- ItemAcesso aberto (Open Access)Alterações da microbiota conjuntival e palpebral após uso tópico de lomefloxacina e tobramicina na cirurgia de catarata e cirurgia refrativa(Conselho Brasileiro de Oftalmologia, 2002-01-01) Hofling-Lima, Ana Luisa [UNIFESP]; Farah, Michel Eid [UNIFESP]; Montenegro, Luciano [UNIFESP]; Alvarenga, Lênio Souza; Chalita, Maria Regina Catai; You, Maria Cecília Zorat [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: To determine the changes in the conjunctival and palpebral flora after topical use of 0.3% lomefloxacin and 0.3% tobramycin eye drops in the preoperative management of patients submitted to cataract and refractive surgery and to assess the chemosensitivity of bacterial isolates from the conjunctiva and eyelid to these antibiotics. Methods: A prospective study of the conjunctival and palpebral flora of patients submitted to cataract or to refractive (PRK or LASIK) surgery was performed. An analysis of the conjunctival and palpebral flora was carried out in patients before surgery without prophylaxis, after surgery during the use of prophylaxis (0.3% lomefloxacin or 0.3% tobramycin qid) and after discontinuation of the antibiotic. Results: Tobramycin and lomefloxacin reduced the number of positive cultures in specimens from the conjunctiva and eyelid of individuals submitted to cataract and refractive surgery. In both groups, isolated microorganisms displayed a greater resistance to tobramycin. In the group submitted to cataract surgery, patients treated with prophylactic tobramycin showed a slower recovery of the flora after discontinuation of the antibiotic than those treated with lomefloxacin, with the opposite occurring in the group submitted to refractive surgery. Conclusion: Both lomefloxacin and tobramycin were effective in reducing conjunctival and palpebral flora during drug administration. This reduction was more marked in the conjunctiva. Among the various bacterial isolates, there was a greater resistance to tobramycin than to lomefloxacin. The use of antibiotics reduced the incidence of positive cultures more for the conjunctiva than the eyelids.
- ItemAcesso aberto (Open Access)Contaminação de frascos de colírios de soro autólogo(Conselho Brasileiro de Oftalmologia, 2001-02-01) Hofling-Lima, Ana Luisa [UNIFESP]; Lima Filho, Acácio Alves de Souza [UNIFESP]; Batistoso, José Antonio; Kawamura, Débora; Chalita, Maria Regina Catai [UNIFESP]; Alves, Leandro Siqueira [UNIFESP]; Farah, Michel Eid [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: To evaluate bacterial contamination of autologous serum eyedroppers after topical use. Methods: Autologous serum eyedroppers used for ocular surface diseases were submitted to culture after topical use. A total of 127 eyedroppers was cultivated after the patients had used the drops and returned them to the laboratory. Results: We found that 76.03% of the eyedroppers were contaminated. Nine-two microorganisms were found: coagulase-negative Staphy-lococcus (35.86%), Alcaligenes sp (21.73%), Klebsiella sp (20.65%) and Bacillus sp (9.78%). Conclusion: The eye-dropper contamination can be caused by normal flora and by skin and environmental microorganisms. These results show the contamination risk at the moment of instillation. Further research will be done to evaluate fungal contamination and to correlate the patient's normal flora with microorganisms found in the eyedroppers.
- ItemAcesso aberto (Open Access)Estudo do raspado de conjuntiva e margem palpebral de pacientes tratados de retinoblastoma: etapa I - Microflora aeróbica(Conselho Brasileiro de Oftalmologia, 2002-03-01) Santos, Maria Carmen Menezes; Santos, Gilda Da Cunha [UNIFESP]; Vozza, João Antônio; Medeiros, Nivaldo; Erwenne, Clélia Maria; Universidade Federal de São Paulo (UNIFESP); Fundação Antônio Prudente Hospital A. C. Camargo Laboratório IPACE; Fundação Antônio Prudente Hospital A. C. Camargo Departamento de Patologia Clínica; Fundação Antônio Prudente Hospital A. C. Camargo Serviço de OftalmologiaPurpose: Conjunctival discharge is a common complaint among patients treated for retinoblastoma who underwent enucleation and radiotherapy. Lack of data about the microflora in those patients motivated this study. Methods: One hundred and fourteen orbits of 58 patients were examined. The authors present the results of aerobic bacterial and fungal culture of conjunctival and eyelid margin smears, as well as conjunctival cytology, in 4 different conditions: with and without enucleation, with and without radiation. Microorganisms were identified according to the National Committee for Clinical Laboratory Standards, using Gram and Leishman staining. Results: Microorganisms were more frequent in irradiated anophthalmic sockets, both in the conjunctiva (54.5%) and eyelid margin (63.6%); however the difference was not statistically significant. On the other hand, in orbits with ocular bulbi, the radiation, in the late phase, represented a limiting factor for the presence of microorganims in the conjunctiva and eyelid margin, but without a statistically significant difference. The coagulase-negative Staphylococcus was the most frequent microorganism in the conjunctiva and eyelid margin in all groups. There was fungus growth, Candida tropicalis, in only one case. Conclusions: Topical antibiotics in patients treated for retinoblastoma who underwent enucleation, with or without radiotherapy, are suggested.