Navegando por Palavras-chave "CREB-1"

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    LONG-TERM TREATMENT WITH STANDARDIZED EXTRACT of GINKGO BILOBA L. ENHANCES the CONDITIONED SUPPRESSION of LICKING in RATS BY the MODULATION of NEURONAL and GLIAL CELL FUNCTION in the DORSAL HIPPOCAMPUS and CENTRAL AMYGDALA
    (Elsevier B.V., 2013-04-03) Oliveira, D. R. [UNIFESP]; Sanada, P. F. [UNIFESP]; Filho, A. C. S. [UNIFESP]; Conceicao, G. M. S. [UNIFESP]; Cerutti, J. M. [UNIFESP]; Cerutti, S. M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Our group previously demonstrated that short-term treatment with a standardized extract of Ginkgo biloba (EGb) changed fear-conditioned memory by modulating gene expression in the hippocampus, amygdaloid complex and prefrontal cortex. Although there are few controlled studies that support the long-term use of EGb for the prevention and/or treatment of memory impairment, the chronic use of Ginkgo is common. This study evaluated the effects of chronic treatment with EGb on the conditioned emotional response, assessed by the suppression of ongoing behavior and in the modulation of gene and protein expression. Male adult Wistar rats were treated over 28 days and assigned to five groups (n = 10) as follows: positive control (4 mg kg(-1) Diazepam), negative control (12% Tween 80), EGb groups (0.5 and 1.0 g kg(-1)) and the naive group. the suppression of the licking response was calculated for each rat in six trials. Our results provide further evidence for the efficacy of EGb on memory. for the first time, we show that long-term treatment with the highest dose of EGb improves the fear memory and suggests that increased cAMP-responsive element-binding protein (CREB)-1 and glial fibrillary acidic protein (GFAP) mRNA and protein (P < 0.001) in the dorsal hippocampus and amygdaloid complex and reduced growth and plasticity-associated protein 43 (GAP-43) (P < 0.01) in the hippocampus are involved in this process. the fear memory/treatment-dependent changes observed in our study suggest that EGb might be effective for memory enhancement through its effect on the dorsal hippocampus and amygdaloid complex. (C) 2013 Published by Elsevier B.V. on behalf of IBRO.
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    Neuromodulatory property of standardized extract Ginkgo biloba L. (EGb 761) on memory: Behavioral and molecular evidence
    (Elsevier B.V., 2009-05-07) Oliveira, Daniela R. [UNIFESP]; Sanada, Priscila F. [UNIFESP]; Saragossa Filho, A. C. [UNIFESP]; Innocenti, L. R.; Oler, Gisele [UNIFESP]; Cerutti, Janete M. [UNIFESP]; Cerutti, Suzete M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Sao Francisco
    Although it has been suggested that the standardized Ginkgo biloba leaf extract (Egb 761) may have a beneficial effect on memory, the cellular and molecular changes that underlie this process are not yet well defined. the present study evaluated the effects of acute (one dose) or subacute treatments (one daily dose/seven days) with EGb 761 (0.5 g kg(-1) and 1.0 g kg(-1)) on rats submitted to a conditioned emotional response (CER) in comparison with positive (4 mg kg(-1) Diazepam) and negative (12% Tween 80) control groups. To this end, eighty (n = 10/group) adult, male, Wistar rats (+/- 250-300 g) were used in an off-baseline CER procedure. We here observed that the rats submitted to an acute and subacute EGb 761 treatments had acquisition of fear conditioning. Additionally, we investigate if the expression of genes previously associated with classical conditioning (CREB-1 and GAP-43) and new candidate genes (GFAP) are modulated following EGb 761 acute treatment. CREB-1, GAP-43 and GFAP mRNA and protein expressions were evaluated using both quantitative PCR (qPCR) and immunohistochemical analysis, respectively. We here show, for the first time, that EGb 761 modulated GAP-43, CREB-1 and GFAP expression in the prefrontal cortex, amygdala and hippocampus. We observed an underexpression of GAP-43 in all structures evaluated and over-expression of GFAP in the amygdala and hippocampus following acute G. biloba treatment when compared to control group (Tween; p<0.01). GAP-43 expression was decreased in prefrontal cortex and hippocampus in the subacute treatment with EGb 761. Subacute treatment with EGb 761 lead to a decreased CREB-1 in mPFC (p<0.001) and increased in the hippocampus to 1.0 g kg(-1) G. biloba group (p<0.001). the results obtained from immunohistochemical analysis support our aforementioned findings and revealed that the changes in expression occurred within specific regions in the areas evaluated. All together, our findings not only provide new evidence for a role of EGb 761 on memory but also identify molecular changes that underlie the fear memory consolidation. (C) 2008 Elsevier B.V. All rights reserved.