Navegando por Palavras-chave "Chagas Disease"
Agora exibindo 1 - 9 de 9
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosCardiac Magnetic Resonance-Verified Myocardial Fibrosis in Chagas Disease: Clinical Correlates and Risk Stratification(Arquivos Brasileiros Cardiologia, 2016) Uellendahl, Marly [UNIFESP]; Siqueira, Maria Eduarda Menezes de [UNIFESP]; Calado, Eveline Barros [UNIFESP]; Kalil-Filho, Roberto; Sobral, Dario; Ribeiro, Clebia; Oliveira, Wilson; Martins, Silvia; Narula, Jagat; Rochitte, Carlos EduardoBackground: Chagas disease (CD) is an important cause of heart failure and mortality, mainly in Latin America. This study evaluated the morphological and functional characteristics of the heart as well the extent of myocardial fibrosis (MF) in patients with CD by cardiac magnetic resonance (CMR). The prognostic value of MF evaluated by myocardialdelayed enhancement (MDE) was compared with that via Rassi score. Methods: This study assessed 39 patients divided into 2 groups: 28 asymptomatic patients as indeterminate form group (IND)
- ItemSomente MetadadadosDoença de Chagas experimental: tentativas de imunização protetora por inoculação previa com flagelos monoxênicos(Universidade Federal de São Paulo (UNIFESP), 1975) Takehara, Harumi Ando [UNIFESP]; Albuquerque, Ivan da Mota e [UNIFESP]
- ItemSomente MetadadadosEstudo comparativo de achados anatomopatotógicos de biópsias endomiocárdicas em transplante cardíaco de pacientes chagásicos e não chagásicos(Universidade Federal de São Paulo (UNIFESP), 1998) Ishigai, Marcia Marcelino de Souza [UNIFESP]; Patrício, Francy Reis da Silva [UNIFESP]Estudamos 293 biopsias endomiocardicas (BEMS) de 33 pacientes transplantados cardiacos chagasicos (C) e nao chagasicos (NC) comparando as alteracoes histopatologicas desenvolvidas pos-Tx em ambos os grupos. O objetivo do estudo foi avaliar se pacientes do grupo C, sob regime especial de imunossupressao, responderiam aos estimulos imunologicos de rejeicao de modo semelhante ao do grupo NC e, diante de recidiva da miocardite chagasica, o padrao histologico diferiria do observado na fase pre-Tx e na rejeicao. O numero medio de BEMs e o tempo de acompanhamento foi semelhante para os dois grupos. Nao houve diferenca quanto ao efeito 'Quilty' nem relacao deste com a intensidade da rejeicao. O mesmo foi observado para isquemia miocardica, comum nas duas primeiras semanas pos-Tx, fibrose miocardica e miocitoiise. Hipertrofia de miocitos foi achado comum nos dois grupos sendo mais frequente em BEMs do grupo NC. A incidencia de episodios de rejeicao foi semelhante nos dois grupos embora maior numero de pacientes do grupo C tenham desenvolvido rejeicoes grau 3-4 (ISHLT). Vasculite arterial ocorreu em dois pacientes chagasicos, um dos quais faleceu. Recidiva da doenca de Chagas ocorreu cinco vezes em quatro pacientes com comprometimento do miocardio em duas. Celulas inflamatorias foram quantificadas usando-se tecnica de imunoperoxidase com marcacao por anticorpos CD4 e CD8. Nao houve diferenca entre os grupos quanto a media de linfocitos T CD4+, CD8+ e da relacao CD4+/CD8+ na rejeicao.. Proporcao semelhante foi observada nos dois casos de recidiva da miocardite chagasica. Niveis inferiores de linfocitos I- CD4+ foram encontrados na miocardite chagasica cronica (coracao do receptor) com relacao CD4+/CD8+ significantemente mais baixa. A sobrevida em um ano foi maior para o grupo NC e a mortalidade esteve relacionada a rejeicao em 75 por cento dos casos. Concluimos que pacientes chagasicos mostraram-se mais suscetiveis a rejeicoes mais intensas. Entretanto, o padrao histopatologico do processo foi similar em ambos os grupos o que indica que a condicao inflamatoria pre-Tx nao altera a resposta do hospedeiro em episodio de rejeicao subsequente. Por outro lado, a composicao linfocitaria na miocardite do grupo C pos-Tx com ou sem parasitas, mostrou-se similar a desenvolvida no grupo NC diferindo da miocardite chagasica pre-Tx.. Este aspecto sugere que o diagnostico de recidiva da infeccao em BEM deva estar baseado na deteccao do parasita e/ou seus antigenos
- ItemAcesso aberto (Open Access)Migração interna e a distribuição da mortalidade por doença de Chagas, Brasil, 1981/1998(Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz, 2006-10-01) Drumond, João Augusto Guimarães; Marcopito, Luiz Francisco [UNIFESP]; Universidade Estadual de Montes Claros; Universidade Federal de São Paulo (UNIFESP)In Brazil, mortality from Chagas disease occurs even in regions classified as free of vector transmission. Because death rates refer to residents, and considering that a huge migratory movement has occurred inside the country, this study was intended to quantify the contribution of Brazilian internal migration to overall mortality from Chagas disease, from 1981 to 1998. If the PAHO Southern Cone Initiative actually achieved its objectives, one could expect declining death rates and increasing age at death from this cause. The results showed that out of 68,936 deaths in Brazilians with known birthplace, 32,369 (32%) occurred in people born in States other than those of their current residence (range: from 0.3% in Rio Grande do Sul to 100% in Roraima and Amapá). Most (67%) of the deaths in migrants occurred in individuals born in the States of Minas Gerais (51%) and Bahia (16%). Death rates in residents showed a consistent decline in the Southeast, South, and Central West of the country, but not in the Northeast and North, where median age at death was the lowest.
- ItemAcesso aberto (Open Access)Miocárdio não compactado, Doença de Chagas e disfunção: relato de caso(Sociedade Brasileira de Cardiologia - SBC, 2010-07-01) Mello, Ronaldo Peixoto de [UNIFESP]; Szarf, Gilberto [UNIFESP]; Nakano, Edson Minoru [UNIFESP]; Dietrich, Cristiano de Oliveira [UNIFESP]; Cirenza, Claudio [UNIFESP]; De Paola, Angelo Amato Vincenzo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)We report the association between heart disease associated with noncompaction of the left ventricular myocardium (NCLVM) and chronic Chagas' heart disease (CCHD) in a patient with heart failure, ischemic stroke and cardiac arrhythmia. Images typical of NCLVM and CCHD were documented by cardiac magnetic resonance imaging (CMRI).
- ItemSomente MetadadadosPorcelia macrocarpa r. E. Fries (annonaceae): prospecção de novos compostos com potencial atividade antiparasitária(Universidade Federal de São Paulo (UNIFESP), 2019-04-26) Oliveira, Emerson Alves De [UNIFESP]; Lago, João Henrique Ghilardi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Despite the devastating scenario imposed by neglected tropical diseases (NTDs) that affect more than 1.4 billion people worldwide, especially in developing countries, there are no public and private investments for the diagnosis, treatment and research of new therapies. Many of these NTDs are treated with very old, low efficacy drugs that have severe side effects and high toxicity. Brazil has 3 million chronically ill patients suffering from Chagas' disease (CD), an NTD whose etiologic agent is Trypanosoma cruzi. Treatment of CD in the country is performed with a single drug, benznidazole that is ineffective against all stages of the disease. In this context, the present study performed the biomonitoring phytochemical analysis by antiparasitic assays of hexane and dichloromethane extracts of P. macrocarpa seeds, which allowed the isolation of four acetylenic acetogenins (A) (2S, 3R , 4R)-3-hydroxy-4- methyl-2-(n-eicos-11'-in-inyl)butanolide, (B) (2S,3R,4R)-3-hydroxy-4-methyl-2-(neicos- 11'-in-19'-enyl)butanolide, (C) (4R)-3-hydroxy-4-methyl-2-(n-eicos-11'-in-19'- enyl)but-2-enolide (D) 3-hydroxy-4-methylene-2-(n-eicos-11'-in-19'-enyl)but-2- enolide, two of which have not been published in the literature (C and D). All of acetogenins isolates were subjected to antiparasitic testes and two (C and D) were more potent than the standard drug against the amastigotes and trypomastigotes forms of T. cruzi, with IC50 values of 3.6 μM and 27.7 μM (amastigote/trypomastigote) for C and 0.4 μM and 23.0 μM (amastigote/trypomastigote) for D. In addition, all active acetogenins (B-D) were evaluated against NCTC cells, which had no cytotoxicity at the highest concentration (200 μM), except for D, where CC50 was 80.0 μM. The mechanism of action of the most potent acetogenins (C and D) was investigated and the results suggested that the plasma membrane depolarization and the observed change in the electrical potential of the mitochondrial membrane of the parasite may be a result of the lethal action of C and D. Complementary in silico ADMET studies were perfomed with active acetogenins B-D and the following features were indicated: (i) all were suitable for human intestinal absorption (HIA+), (ii) none were substrates of CYP2D6, (vi) except for D, none were substrates for CYP2C9, and (vii) none acted as inhibitors of the following isoforms: CYP3A4, CYP2C9, CYP2C1I9, CYP2D6, or CYP1A2. In addition the active acetogenins B-D are not mutagenic (Ames), carcinogenic, prone to colloidal aggregation. They are also not hERG K+ channel blockers (multi-class) no pan-assay interference compounds (PAINS). Thus, acetogenins C and D can be considered excellent prototypes for the development of drugs, aiming at the effective treatment of Chagas' disease in the acute and chronic phases of the disease.
- ItemSomente MetadadadosResposta das células T CD8+ em modelos de obesidade infectados pelo Trypanosoma Cruzi(Universidade Federal de São Paulo (UNIFESP), 2018-03-19) Correa, Priscila Gontijo [UNIFESP]; Vasconcelos, Jose Ronnie Carvalho de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: Chagas Disease Is An Infection Transmitted By The Intracellular Protozoan Trypanosoma Cruzi. The Specific Immune Response Mediated By Cd8 + T Cells Are Crucial To Control Infection, And Changes In Response Of These Cells Increases The Risk Of Morbidity And Mortality By The Disease. The Excess Of Adipocytes, Characteristic Of Obesity, Potentiates The Inflammatory Process From The Infection, Which Could Interfere In The Response Of The Specific Cd8 + T Cells. Objective: Assess The Response Of The Specific Cd8+ T Cells Generated In The Infection Of The Trypanossoma Cruzi, In Different Models Of Obesity. Material And Methods: Two Murine Models Of Obesity Were Studied, One Genetically Linked And Another Induced By High Fat Diet (Hfd) Consumption. For Genetic Obesity, Ob/Ob Fat And Ob/Ob Lean Mice Were Used Animals. For Hfd-Induced Obesity Model, C57bl/6 Mice Were Fed A High Fat Diet, Containing 39% Of Fat. The Two Models Were Infected With T. Cruzi, And Blood Parasitemia, Survival And Immune Response
- ItemSomente MetadadadosThe substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi(Blackwell Publishing, 2006-06-01) Reis, Flavia Coelho Garcia dos; Judice, Wagner AS; Juliano, Maria Aparecida [UNIFESP]; Juliano, Luiz [UNIFESP]; Scharfstein, Julio; Lima, Ana Paula CD; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Federal de São Paulo (UNIFESP)Papain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates.
- ItemSomente MetadadadosTrypanosoma-cruzi - detection of a circulating antigen in urine of chagasic patients sharing common epitopes with an immunodominant repetitive antigen(Academic Press Inc Jnl-comp Subscriptions, 1993-06-01) Umezawa, Eufrosina Setsu; Shikanai-Yasuda, Maria Aparecida; Silveira, Jose Franco da [UNIFESP]; Cotrim, Paulo Cesar [UNIFESP]; Paranhos, Gláucia da Silva [UNIFESP]; Katzin, Alejandro Miguel; BIOMERIEUX; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)