Navegando por Palavras-chave "Colony count, microbial"

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    Estudo da micobiota em conjuntiva sadia de diabéticos, residentes na área urbana da cidade de São Paulo - Brasil
    (Conselho Brasileiro de Oftalmologia, 2006-02-01) Andrade, Alfredo José Muniz de [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Godoy, Patricio [UNIFESP]; Gompertz, Olga Fischman [UNIFESP]; Bonfim, Sabrina de Souza [UNIFESP]; Andrade, Francisco Eudes Muniz de; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Pernambuco
    PURPOSE: To determine the mycobiota of the healthy conjunctiva in diabetic individuals, according to diabetes type, age, sex, disease time, type of treatment, and stage of diabetic retinopathy of the individuals. To identify the anemophilus mycobiota in the sampling rooms. METHODS: A cross-sectional study was carried out on 803 diabetics who reside in the urban area of São Paulo-SP/Brazil. Sabouraud's dextrose agar culture with chloramphenicol was used for primoisolation, and the key of De Hoog was used to identify filamentous fungi. RESULTS: Of the evaluated diabetics, 6.6% (53/803) presented type 1 diabetes and 93.4% (750/803) type 2. The positive cultures for fungi in the conjunctiva of diabetics was 4.2% (34/803), with 1.9% (1/53) in type 1 diabetics and 4.4% (33/740) in type 2 diabetics (p=0.720). With respect to the presence or not of isolated fungi, there was no statistically significant association regarding age (p=0.575), sex (p=0.517), disease time (p=0.633), type of treatment (p=0.422), and diabetic retinopathy stage (p=0.655) of the tested individuals. The identified fungi were all filamentous: Aspergillus spp. represented 59.5% (25/42) of isolations and 47.6% (20/42) of isolated species were Aspergillus niger. Growth of anemophilus fungi occurred in the air of the room and coincidences were observed between the isolated species from the air and those from the conjunctiva. CONCLUSIONS: Presence of mycobiota in healthy conjunctivas of diabetics was identified, with no significant association between the greater number of positive fungi isolations and the type of diabetes, age, sex, disease type, type of treatment, and stage of diabetic retinopathy. In the collection rooms, anemophilus mycobiota was identified.
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    Microbiota conjuntival em pacientes com alergia ocular
    (Conselho Brasileiro de Oftalmologia, 2005-12-01) Libório, Alexandre Mattoso; Nishiwaki-Dantas, Maria Cristina [UNIFESP]; Mimica, Lycia Mara Jenne; Dantas, Paulo Elias Correa; Hofling-Lima, Ana Luisa [UNIFESP]; Santa Casa de São Paulo Faculdade de Ciências Médicas Departamento de Oftalmologia; Universidade Federal de São Paulo (UNIFESP); Santa Casa de São Paulo Faculdade de Ciências Médicas Laboratório de Microbiologia; Santa Casa de São Paulo Faculdade de Ciências Médicas setor de Córnea e Doenças Externas
    PURPOSE: To evaluate de presence of conjunctival aerobic microbiota in patients with ocular allergy as compared to a control group. METHODS: One hundred and thirty-three patients were evaluated from April to June 2001 and divided into 2 groups. Sixty-three patients with allergic conjunctivitis (without medication) were in group A and 70 patients from the general outpatient clinic were in group B (control group). Samples from the conjunctival sac of the right eye were collected and cultured in solid media (blood, chocolate and Sabouraud agar). RESULTS: In group A, 30 cultures (47.7%) were positive and 6 (8.6%) in group B. Seven bacteria were isolated from group A and 4 from group B. Statistical analysis revealed significant association between positive cultures and allergic conjunctivitis. CONCLUSION: Bacterial microbiota was more frequently found in patients with ocular allergy.