Navegando por Palavras-chave "CuPcTs"

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    Impedance sensing of DNA hybridization onto nanostructured phthalocyanine-modified electrodes
    (Pergamon-Elsevier Science Ltd, 2016) Manzanares-Palenzuela, C. L.; Fernandes, Edson Giuliani Ramos [UNIFESP]; Lobo-Castanon, M. J.; Lopez-Ruiz, B.; Zucolotto, V.
    DNA detection is still undergoing major innovations in pursuit of low-cost and simple approaches for decentralized applications. Label-free sensing of DNA hybridization via impedance measurements is a popular strategy to fulfil the goals of cost-efficiency and simplicity. Several materials are often reported for electrode modification to improve the sensitivity of impedance-based sensors. Herein we evaluate the electronic properties of copper phthalocyanine tetrasulfonate (CuPcTs) in Layer-by-Layer (LbL) films for impedimetric sensing of DNA hybridization using silanized Fluorine-doped Tin Oxide (FTO) electrodes. 1 to 5 bilayers were prepared by alternate immersion of the substrate in CuPcTs and poly(allylamine hydrochloride) (PAH). DNA probe immobilization was carried out electrostatically onto the last PAH layer, followed by hybridization with the target sequence leading to the formation of a partial double stranded (pds) structure onto the films. Impedance decreased after hybridization proportionally with the concentration of the target sequence at picomolar levels. Not only are these findings useful as a potential biosensing strategy, but also leave an open question about the electronic and synergistic properties of DNA interacting with different materials and surfaces. (C) 2016 Elsevier Ltd. All rights reserved.