Navegando por Palavras-chave "Culture Media"

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    Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-10-01) Machado, Natasha Mendonça [UNIFESP]; Duailibi, Silvio Eduardo [UNIFESP]; Santos, Jennifer Adriane dos; Penna, Vanessa; Ferreira, Lydia Masako [UNIFESP]; Duailibi, Monica Talarico [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Science and Technology Institute
    PURPOSE:To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation.METHODS:Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity.RESULTS:A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate.CONCLUSION:Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.
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    Hemoderivados como suplemento no meio de cultivo para células-tronco dentárias humanas
    (Universidade Federal de São Paulo (UNIFESP), 2011-07-27) Pisciolaro, Ricardo Luiz [UNIFESP]; Duailibi, Silvio Eduardo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: One among many aims of medicine is to overcome injuries inflicted to the organism by diseases, aging and trauma, re-establishing the usual functions. About tissues losses, several authors claim that the ideal replacement is the healthy tissue itself, originated from the same source or developed by Tissue Engineering (TE). However, much research is needed before in vivo application. Objective: To evaluate three different kinds of sera supplies used in stem cell culture media, as to cellular proliferation and cellular injuries on dental stem cell. Methods: Five experiments were made utilizing incompletely developed third molar teeth. After enzymatic digestion, the adult stem cells were cultivated in four different kinds of culture media. Medium I, serum free (SF); medium II, supplied with FBS (heterologous serum- HeS); medium III, supplied with homologous human serum (homologous serum- HoS) and medium IV, supplied with autologous human serum (autologous serum . AuHS).These cultures were analyzed comparatively as to cellular proliferation; they were submitted Von Kossa (VK) and Alizarin Red (AR) markers tests for four weeks (checked weekly), and each two weeks checked for Colonies Forming Unities (CFUs). On the 28th day, all four cultures were submitted to comet assay, and were inspected for possible cellular DNA injuries. The results underwent a non-parametric statistical Friedman fs variance test, with significance (p) . 5%. Results: Culture medium IV reached a cellular proliferation rate higher than medium I, showing a significant result (p*=0,0074). Culture medium II presented a superior proliferation result than medium I, and similar to medium III, although neither of them presented significant result when compared to medium IV. The comet assay fs results showed minor cellular DNA injury in the medium IV cultures, when compared to medium II and III cultures. The CFUs were numerous in the media IV and III cultures, respectively, and there was higher mineralization rate in the medium IV than in the media II and III. Conclusion: The culture medium supplied with AuHS significantly improved cellular proliferation. Human sera proved to be a viable supply to human dental stem cell culture.