Navegando por Palavras-chave "Diarrheagenic E. coli"

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    Captive wild birds as reservoirs of enteropathogenic E-coli (EPEC) and Shiga-toxin producing E-coli (STEC)
    (Soc Brasileira Microbiologia, 2017) Sanches, Lilian Aparecida; Gomes, Marcelo da Silva; Friciello Teixeira, Rodrigo Hidalgo; Vieira Cunha, Marcos Paulo; Xavier de Oliveira, Maria Gabriela; Midolli Vieira, Monica Aparecida [UNIFESP]; Tardelli Gomes, Tania Aparecida [UNIFESP]; Knobl, Terezinha
    Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of Sao Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp (EPEC), stxl and stx2 for STEC. The results of these tests revealed 23/401 (5.74%) positive strains for eae gene, 16/401 positive strains for the bfp gene (3.99%) and 3/401 positive for stx2 gene (0.75%) distributed among the orders of Psittaciformes, Strigiformes and Columbiformes. None of strains were positive for stxl gene. These data reveal the infection by STEC, typical and atypical EPEC in captive birds. The frequency of these pathotypes is low and restricted to few orders, but the data suggest the potential public health risk that these birds represent as reservoirs of diarrheagenic E. coli. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
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    Etiology of childhood diarrhea in the northeast of Brazil: significant emergent diarrheal pathogens
    (Elsevier B.V., 2010-01-01) Ramos Moreno, Ana Carolina; Fernandes Filho, Antonio; Tardelli Gomes, Tania do Amaral [UNIFESP]; Ramos, Sonia T. S.; Montemor, Liana P. G.; Tavares, Vanessa C.; Santos Filho, Lauro dos; Irino, Kinue; Martinez, Marina Baquerizo; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Univ Fed Paraiba; Adolfo Lutz Inst
    In a study conducted in Joao Pessoa, northeast of Brazil, 2344 Escherichia coli isolated from 290 infants with diarrhea and 290 healthy matched controls were analyzed for virulence traits. Enteroaggregative E. coli (EAEC) was the most prevalent pathogen associated to acute diarrhea. Based on the results of colony blot hybridization, serotyping, and HEp-2 cell adherence assays, strains were separated in categories as typical enteropathogenic E. coli (EPEC) (1.7%), atypical EPEC (a-EPEC) (9.3%), EAEC (25%), enterotoxigenic E. coli (10%), and enteroinvasive E. coli (EIEC) (1.4%). No enterohemorrhagic E. coli strains were isolated. Other enteropathogens were found, including Salmonella (7.9%), Shigella spp. (4.1%), thermophilic Campylobacter spp. (2.4%), Giardia lamblia (9.3%), and Entamoeba histolytica (5.8%). All enteropathogens were associated with diarrhea (P < 0.01). However, the association was lower for EPEC and EIEC (P < 0.03). Different pathogens associated with diarrhea may have been changing in Brazil where EAEC and a-EPEC seem to be the most prevalent pathogens among them. (C) 2010 Elsevier Inc. All rights reserved.
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    High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
    (Biomed Central Ltd, 2017) Spano, Liliana Cruz; da Cunha, Keyla Fonseca; Monfardini, Mariane Vedovatti [UNIFESP]; Bergamaschi Fonseca, Rita de Cassia; Affonso Scaletsky, Isabel Christina [UNIFESP]
    Background: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adults