Navegando por Palavras-chave "EPR cells"

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    Implante celular do epitélio pigmentar da retina derivado de células-tronco embrionárias em um modelo murino: princípios para a terapia regenerativa celular em humanos
    (Universidade Federal de São Paulo (UNIFESP), 2016-11-29) Ribeiro, Ramiro Magalhães [UNIFESP]; Maia, Mauricio [UNIFESP]; http://lattes.cnpq.br/6377105744231862; http://lattes.cnpq.br/9793000308103027; Universidade Federal de São Paulo (UNIFESP)
    Objective: to demonstrate the principle of cellular therapy after the implantation of retinal pigmented epithelial cells (RPE) derived from embryonic stem-cells (here denominated hESC-RPE) seeded in a parylene-C membrane in a murine animal model. Method: after the implant of the cell into the murine animal, four different studies were done. In the first study, analysis of histology and optical coherence images (OCT) were performed immediately (n=6) or seven days (n=5) after the implant. In the second study, monthly infrared imagens and OCT were obtained in animals that received the implant (n=14) and control group (parylene-C membrane without the presence of cells, n=14). The images were correlated and compared with histology findings. In the third study, histological analyses were performed in ocular and extra-ocular tissue in an immunocompromised animal (n=36). The cellular survival rate and the tumor formation were compared with a group of animals (n=33) that received the implant of hESC-RPE as a cell suspension. In the fourth study, the visual behavioral response and quantification of the photoreceptors were analyzed in animals with progressive degenerative retina disease after the implant of cells (n=20) and compared with a surgical control group (n=10) and untreated group (fellow eye). Animals implanted with the hESC-RPE as a cell suspension (n=18) were also evaluated. Results: OCT images, microscopy and histology demonstrated the successful surgical implantation leading to a small cell lost (less than 2%). Infrared images identified in-vivo areas corresponded with the implanted cells and those findings were confirmed by histology and specific markers. No areas corresponding with hESC-RPE was identified in animals that received the implant of parylene without the cells. Histology analysis demonstrated the cell survival for a period of up to one year and confirmed the non-formation of ocular and extra-ocular tumor. The survival rate was superior in the animals that received the implant of hESC-RPE/parylene compared with the injection group. The animals implanted with the hESC-RPE cells demonstrated a better visual response between the 4th and 25th weeks after the implant. Afterwards, no visual response was detected in any of the animals. The hESC-RPE also led to a better preservation of the photoreceptors. Conclusion: The implant of hESC-RPE cells seeded in a parylene-C membrane into the subretinal space was viable, imagining studies detected the presence of in-vivo cells and its correlation with histology findings were positive, the cells survived for a period up to one year and did not lead to formation of tumors and the implantation of the cells were able to slow the progression of visual lost and retina degeneration.