Navegando por Palavras-chave "Electroconvulsive shock"

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    Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice
    (Biomed Central Ltd, 2013-09-06) Ferrazoli, Eneas Galdini [UNIFESP]; Blanco, Miriam Marcela [UNIFESP]; Bittencourt, Simone [UNIFESP]; Bachi, Andre Luis Lacerda [UNIFESP]; Bahia, Luciana; Soares, Milena Botelho Pereira; Ribeiro-dos-Santos, Ricardo; Mello, Luiz Eugenio [UNIFESP]; Longo, Beatriz Monteiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fundacao Oswaldo Cruz; Hosp Sao Rafael
    Background: Bone marrow is an accessible source of progenitor cells, which have been investigated as treatment for neurological diseases in a number of clinical trials. Here we evaluated the potential benefit of bone marrow cells in protecting against convulsive seizures induced by maximum electroconvulsive shock (MES), a widely used model for screening of anti-epileptic drugs. Behavioral and inflammatory responses were measured after MES induction in order to verify the effects promoted by transplantation of bone marrow cells. To assess the anticonvulsant effects of bone marrow cell transplantation, we measured the frequency and duration of tonic seizure, the mortality rate, the microglial expression and the blood levels of cytokine IL-1, IL-6, IL-10 and TNF-alpha after MES induction. We hypothesized that these behavioral and inflammatory responses to a strong stimulus such as a convulsive seizure could be modified by the transplantation of bone marrow cells.Results: Bone marrow transplanted cells altered the convulsive threshold and showed anticonvulsant effect by protecting from tonic seizures. Bone marrow cells modified the microglial expression in the analyzed brain areas, increased the IL-10 and attenuate IL-6 levels.Conclusions: Bone marrow cells exert protective effects by blocking the course of electroconvulsive seizures. Additionally, electroconvulsive seizures induced acute inflammatory responses by altering the pattern of microglia expression, as well as in IL-6 and IL-10 levels. Our findings also indicated that the anticonvulsant effects of these cells can be tested with the MES model following the same paradigm used for drug testing in pharmacological screening. Studies on the inflammatory reaction in response to acute seizures in the presence of transplanted bone marrow cells might open a wide range of discussions on the mechanisms relevant to the pathophysiology of epilepsies.
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    O uso de células-tronco adultas em modelos experimentais de crises convulsivas
    (Universidade Federal de São Paulo (UNIFESP), 2010-05-26) Ferrazoli, Eneas Galdini [UNIFESP]; Longo, Beatriz Monteiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    In Brazil, it is estimated that epilepsy affects from 2% of the population, about three million people at different ages and social classes. The use of antiepileptic drugs is the most common form of seizure control, although about 50- 70% of patients with epilepsy are refractory to medication. Because of difficulties in finding effective treatments, it is crucial to develop new therapies through new research fronts. Recent findings about the ability of pluripotent cells for regeneration of the adult nervous system allow to investigate the potential use of adult stem cells from bone marrow and other sources in neuronal regeneration and functional recovery of the epileptic brain. The aim of this study was to evaluate the potential of adult stem cells in protecting the induction of seizures, and possible decreased frequency of seizures. Using two experimental models of seizures, the maximum electroshock seizure acute model and the lithium-pilocarpine chronic model, and the transplantation of whole cells and mesenchymal bone marrow and skeletal muscle, we evaluated the potential of these cells to raise the seizure threshold and reverse the disease-induced damage. Our results indicated that the transplanting adult stem cells was not able to increase the seizure threshold in the acute model, as well as in the chronic model where the cells could not reduce spontaneous epileptic seizures. However, transplantation of adult stem cells is promising, indicating a reduction in mortality in the acute model. Moreover, in the chronic model we have shown the migration of transplanted cells to regions where there is massive neuronal death, indicating a potential treatment for a tissue recovery.