Navegando por Palavras-chave "Endothelial dysfunction"
Agora exibindo 1 - 6 de 6
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)Association of urinary 90 kDa angiotensin- converting enzyme with family history of hypertension and endothelial function in normotensive individuals(Associação Brasileira de Divulgação Científica, 2008-05-01) Teixeira, A.m.s. [UNIFESP]; Plavnik, Frida Liane [UNIFESP]; Fernandes, Fernanda Barrinha [UNIFESP]; Marson, Odair [UNIFESP]; Christofalo, Dejaldo Marcos de Jesus [UNIFESP]; Ajzen, Sergio Aron [UNIFESP]; Sesso, Ricardo de Castro Cintra [UNIFESP]; Franco, Maria do Carmo Pinho [UNIFESP]; Casarini, Dulce Elena [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)We described angiotensin-I-converting enzyme (ACE) isoforms with molecular masses of 190, 90, and 65 kDa in the urine of normotensive offspring of hypertensive subjects. Since they did not appear in equal amounts, we suggested that 90 kDa ACE might be a marker for hypertension. We evaluated the endothelial response in normotensive offspring with or without family history of hypertension and its association with the 90 kDa ACE in urine. Thirty-five normotensive subjects with a known family history of hypertension and 20 subjects without a family history of hypertension, matched for age, sex, body weight, and blood pressure, were included in the study. Endothelial function was assessed by ultrasound and a sample of urine was collected for determination of ACE isoforms. In the presence of a family history of hypertension and detection of 90 kDa ACE, we noted a maximal flow mediated dilation of 12.1 ± 5.0 vs 16.1 ± 6.0% in those without a previous history of hypertension and lacking urinary 90 kDa ACE (P < 0.05). In subjects with a family history of hypertension and presenting 90 kDa ACE, there were lower levels of HDL-cholesterol (P < 0.05) and higher levels of triglycerides (P < 0.05). Subjects with 90 kDa ACE irrespective of hypertensive history presented a trend for higher levels of triglycerides and HDL-cholesterol (P = 0.06) compared to subjects without 90 kDa ACE. Our data suggest that the 90 kDa ACE may be a marker for hypertension which may be related to the development of early atherosclerotic changes.
- ItemSomente MetadadadosEfeito do treinamento físico aeróbico sobre a resposta vasomotora de ratas ovariectomizadas(Universidade Federal de São Paulo (UNIFESP), 2014-02-26) Nogueira, Viviane Aparecida Vieira [UNIFESP]; Medeiros, Alessandra [UNIFESP]; http://lattes.cnpq.br/0071198026371230; Universidade Federal de São Paulo (UNIFESP)BP reduction, however, is not yet well established effect of aerobic exercise on vasomotor response in the absence of estrogen. The present study examined the effect of aerobic exercise training on vasomotor response of ovariectomized rats. The sample consisted of female Wistar rats divided to 4 groups: sedentary and trained ovariectomized trained and sedentary (OVX and OVX-TRE) control (SHAM and SHAM-TRE). The results showed that groups OVX and OVX-TRE showed an increase in body mass and flow blood pressure during the weeks after ovariectomy, however, after the 5th of experiment group OVX-TRE showed a reduction in gain body and tail arterial pressure mass when compared to group OVX. After the 7th without experiment, group SHAM-TRE had a reduction in body weight compared to the SHAM group. The group OVX showed a lower endothelium dependent vasodilation, and this response was prevented in the group OVX-TRE by aerobic training. The endothelium dependent vasodilation of the group SHAM-TRE was close to the values of the group SHAM. On the other hand, endothelium independent vasodilation was not different between the groups. The level of reactive oxygen species was higher in the group OVX compared with the other groups (SHAM, SHAM-TRE and OVX-TRE), thus aerobic training was able to reduce the production of reactive oxygen species in the vessel group OVX-TRE. The results suggest that physical training promotes improved body composition and decreasing blood pressure levels of ovariectomized rats, besides contributing to the improvement of vasomotor response and reduced superoxide anion production in the vessel, even in estrogen deficiency.
- ItemAcesso aberto (Open Access)Efeito do treinamento físico aeróbico sobre os níveis de espécies reativas de oxigênio na aorta de ratas ovariectomizadas(Universidade Federal de São Paulo (UNIFESP), 2013-12-17) Sales, Daniele Correia de [UNIFESP]; Medeiros, Alessandra [UNIFESP]; Braga, Viviane Aparecida Vieira Nogueira [UNIFESP]; http://lattes.cnpq.br/9613840344126322; http://lattes.cnpq.br/0071198026371230; http://lattes.cnpq.br/8907272443949264; Universidade Federal de São Paulo (UNIFESP)Sabe-se que a hipertensão arterial é uma doença multifatorial que atinge grande parte da população adulta, principalmente mulheres em idade mais avançada no período da pós-menopausa. Isso se deve a alterações relacionadas ao hipoestrogenismo. O hipoestrogenismo está relacionado com o surgimento do estresse oxidativo nos vasos sanguíneos e consequentemente promove o surgimento da disfunção endotelial, que por sua vez contribui para o desenvolvimento da hipertensão arterial. Sendo assim, o objetivo do presente estudo foi avaliar o efeito do treinamento físico aeróbio sobre os níveis de espécies reativas de oxigênio na aorta de ratas ovariectomizadas. Métodos: Foram estudadas 40 ratas Wistar divididas em 4 grupos: controle sedentárias, controle treinadas, ovariectomizadas sedentárias e ovariectomizadas treinadas. Todas as ratas passaram pela cirurgia de ooforectomia bilateral, porém o grupo controle não teve seus ovários removidos. Após a cirurgia foram submetidas a um treinamento físico de intensidade moderada, uma hora por dia, cinco vezes por semana, durante oito semanas. Ao final desse período foram sacrificadas, a aorta foi retirada e congelada para análise das medidas de espécies reativas de oxigênio (EROs). Resultados: Ao final do período de experimento, pode-se observar que as ratas dos grupos ovariectomizados apresentaram maior quantidade de EROs (superóxido) na aorta, quando comparadas as ratas do grupo controle. No entanto, pode-se observar também que o treinamento físico aeróbico foi capaz de atenuar esse índice mesmo na condição de deficiência de estrogênio. Conclusão: O treinamento físico aeróbico foi capaz de atenuar o índice de EROs na aorta de ratas ovariectomizadas.
- ItemAcesso aberto (Open Access)Efeitos da suplementação de vitamina D sobre a pressão arterial, metabolismo glicídico, responsividade vascular e função renal de ratos espontaneamente hipertensos submetidos à dieta hiperlipídica palatável(Universidade Federal de São Paulo (UNIFESP), 2016-10-31) Geraldini, Simone [UNIFESP]; Cesaretti, Mario Luis Ribeiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Background: Vitamin D insufficiency has been associated with several diseases such as obesity, diabetes mellitus and hypertension. However, the cause-effect relationship of vitamin D supplementation on dysfunctions and abnormalities of metabolic syndrome has not been clearly elucidated. Objective: Analyze the effects of cholecalciferol supplementation on blood pressure, glucose metabolism, body weight, renal function and vascular relaxation in spontaneously hypertensive rats (SHR), with palatable hyperlipidic diet (PHD)-fed. Methods: Male 12-weeks-old SHR rats were divided into four groups: control SHR (SHR-C), SHR+PHD (SHR-OB), SHR+vit.D (SHR-VD), SHR+PHD+vit.D (SHR-OBVD). Cholecalciferol supplementation was performed by gavage (1000 UI/kg/daily), and the animals were treated for 12 weeks. Weekly, body weight and tail blood pressure (TAP) were measured. At the end of 12 weeks, Oral Glucose Tolerance Test (GTT) and Insulin Tolerance Test (ITT) were performed. Proteinuria was determined in the baseline and the end period, 25-hidroxivitamina D serum and clearance of creatinine were determined in the end of treatment. The vascular endothelium-dependent function was evaluated in isolated rings of the thoracic aortas, also the relative visceral fat weight was performed. Results: The SHR-OB group presented lower body weight gain compared to the SHR-C and SHR-OBVD groups (p=0,02). However, both treated groups with PHD showed higher epididimal fat than the groups standard rat chow-fed (p<0,001). The vitamin D was not able to reduce final TAP as well as shift maximal responses of endothelium-dependent vascular relaxation. Besides, vitamin D treatment attenuated insulin resistance only on SHR-VD group, in the same way decreased the proteinuria only in this group (p=0,001). Conclusion: An oral supplementation of vitamin D can be an adequate strategy for delay the development of glucose metabolism dysfunctions in metabolic syndrome when associated with a balanced diet, avoiding therefore the loss of renal function.
- ItemAcesso aberto (Open Access)Homocisteína(Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2004-10-01) Neves, Lindalva Batista [UNIFESP]; Macedo, Danielle Mazziero [UNIFESP]; Lopes, Antonio Carlos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Homocysteine, formed from hepatic methionine, is metabolized through the pathways of demethylation and transsulfuration. Its plasmatic and urinary values reflect the cell synthesis. Its determination after fasting and increased infusion of methionine shows the differences of these two metabolic pathways, mainly when it is related to genetic diseases. Hyperhomocysteinemia has been associated with a higher risk of vascular thrombotic events. Several authors suggest a causal relationship between these events independently of other risk factors for vascular diseases. Decrease in plasmatic homocysteine to normal levels is followed by a significant reduction on the incidence of vascular thrombotic events. The correlation between the liver and homocysteine is becoming more important because of the recent findings that alterations of lipoproteins and methionine clearance are common in patients with hepatocellular and canalicular chronic liver disease. The treatment of hiperhomocysteinemia is based on the supplementation of folic acid and vitamins B6 and B12.
- ItemSomente MetadadadosIntrauterine growth restriction-induced deleterious adaptations in endothelial progenitor cells: possible mechanism to impair endothelial function(Cambridge Univ Press, 2017) Oliveira, Vanessa [UNIFESP]; Souza, Livia Victorino de [UNIFESP]; Fernandes, Tiago; Silva Júnior, Sebastião Donato; Carvalho, Maria Helena Catelli de; Akamine, Eliana Hiromi; Michelini, Lisete Compagno; Oliveira, Edilamar Menezes de; Franco, Maria do Carmo Pinho [UNIFESP]Intrauterine growth restriction (IUGR) can induce deleterious changes in the modulatory ability of the vascular endothelium, contributing to an increased risk of developing cardiovascular diseases in the long term. However, the mechanisms involved are not fully understood. Emerging evidence has suggested the potential role of endothelial progenitor cells (EPCs) in vascular health and repair. Therefore, we aimed to evaluate the effects of IUGR on vascular reactivity and EPCs derived from the peripheral blood (PB) and bone marrow (BM) in vitro. Pregnant Wistar rats were fed an ad libitum diet (control group) or 50% of the ad libitum diet (restricted group) throughout gestation. We determined vascular reactivity, nitric oxide (NO) concentration, and endothelial nitric oxide synthase (eNOS) protein expression by evaluating the thoracic aorta of adult male offspring from both groups (aged: 19-20 weeks). Moreover, the amount, functional capacity, and senescence of EPCs were assessed in vitro. Our results indicated that IUGR reduced vasodilation via acetylcholine in aorta rings, decreased NO levels, and increased eNOS phosphorylation at Thr495. The amount of EPCs was similar between both groups; however, IUGR decreased the functional capacity of EPCs from the PB and BM. Furthermore, the senescence process was accelerated in BM-derived EPCs from IUGR rats. In summary, our findings demonstrated the deleterious changes in EPCs from IUGR rats, such as reduced EPC function and accelerated senescence in vitro. These findings may contribute towards elucidating the possible mechanisms involved in endothelial dysfunction induced by fetal programming.