Navegando por Palavras-chave "Environmental factors"

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    Environmental Factors Associated with Psychotropic Drug Use in Brazilian Nightclubs
    (Springer, 2017) Carlini, Claudia [UNIFESP]; Andreoni, Solange [UNIFESP]; Sanchez, Zila M. [UNIFESP]
    The purpose of this study was to identify environmental factors associated with patterns of psychotropic drug use in nightclubs. Mixed methods were used to investigate psychotropic drugs consumption among patrons of 31 nightclubs in So Paulo, Brazil. A total of 1822 patrons at the entrance and exit of the venues and 30 staff members of the nightclubs were interviewed. The observational data were collected through 307 h of observational research using a structured guide to register environmental measures. Psychotropic drug use in nightclubs was classified into three categories (1: no drugs
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    Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells
    (Biomed Central Ltd, 2014-12-20) Romao, Fabiano T. [UNIFESP]; Hernandes, Rodrigo T. [UNIFESP]; Yamamoto, Denise [UNIFESP]; Osugui, Lika [UNIFESP]; Popi, Ana Flavia [UNIFESP]; Gomes, Tania A. T. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    Background: Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. in this study, we evaluated the effect of different glucose (5 and 25 mM) and CO2 (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551-2.Results: We found that a CO2-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551-2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). in addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. the presence of bile salts did not interfere with the adhesion properties of the 1551-2 strain to HeLa cells.Conclusions: Our data suggest that a CO2-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process.