Navegando por Palavras-chave "Esfingomielinase D"

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    Identificação de potenciais antígenos do carrapato Rhipicephalus (Boophilus) microplus para o desenvolvimento de vacinas
    (Universidade Federal de São Paulo (UNIFESP), 2020-07-30) Silva, Fernando Allan Abreu [UNIFESP]; Tanaka, Aparecida Sadae [UNIFESP]; Universidade Federal de São Paulo
    The tick Rhipicephalus (Boophilus) microplus is responsible for considerable economic losses to the livestock sector. The main control method of infestation of this parasite is by acaricides and can result in the selection of resistant ticks, environment and products contamination, between other factors. The vaccine is a more sustainable alternative, but the products available on the market are not efficient in the Brazilian territory. Although the tick genome has not yet been elucidated, transcriptomes and proteomes can indicant potential targets for selection of antigens. Two proteins sequences were selected for this work, a sphingomyelinase D – like and a cathepsin A – like from a tick transcriptome. The DNA sequences that codified these two proteins were cloned into the pET14b expression vector and confirmed by DNA sequencing. The analysis of the translated amino acids of cathepsin A – like showed the absence of amino acids residues important for the activity of this enzyme when compared to others cathepsins A sequences. However, it was not possible to confirm experimentally this data since the recombinant protein was not express in bacterial system. In contrast, sphingomyelinase D – like was expressed in bacteria in large quantities, but in the form of inclusion body. The sphingomyelinase D – like was partially purified in the presence of urea. The analysis of sphingomyelinase D – like primary structure showed a replacement of a histidine for an asparagine in an important position for the enzymatic activity. Therefore, the perspective of this work is to obtain the purified sphingomyelinase D – like and carry out its biochemical and functional characterization.