Navegando por Palavras-chave "Espongina"

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    Biocompatibilidade do colageno marinho (espongina) extraida de esponjas marinhas para utilizacao na area de engenharia tecidual
    (Universidade Federal de São Paulo (UNIFESP), 2020-03-03) Santana, Alan De Franca [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Universidade Federal de São Paulo
    Bone tissue is a specialized system and able to regenerate, but in some situations, which can be a fracture due to over load, accidents, pathology, aging. The context of fracture incidence increases significantly, representing a serious public health problem. Estimating about 6.2 million annual fractures in the United States, and this 10% progress to cases of non-consolidation and pseudarthrosis. Generating an expense of approximately 25 thousand cases in cases of pseudarthrosis. Other therapeutic sources have been investigated in order to assist the evaluation process when consequences arise in the consolidation process. Thus biomaterials are gaining prominence as an alternative in the treatment of fractures, being that of collagen origin one of the most used in the area of tissue engineering for its irritability index, good biodegradation and biocompatibility. However, most of this material is of bovine or porcine origin and may be at risk of transmission and possibility of transmission of viruses and bacterial infections. In addition, it may have been paid for an extraction and manufacture of these sources, reflecting in high prices. For the research non marine environment emerge as an alternative to prospecting new sources of raw material to biomaterials. Among the organisms a sponge, the keyword is a structural component, is a piece called inorganic called Basilica, and a part is called sponge, is an analog of type XIII. Aplyina fulva fluidin, in vitro and in vivo of spp. A characterization made by SEM / EDX, FTIR, pH Assay and Mass. Collagen was used for comparison in pH and mass analysis. The SEM / EDX results were displayed as a PES, showed irregular presence numbers of Al (Aluminum), C (Carbon), O (Oxygen) and S (Sulfur), Ca (Calcium), Cl (Chlorine), Fe ( Iron), Ti (Magnesium), Na (Sodium), P (Phosphorus) and Si (Silicon). The FTIR results were possible observing the peaks referring to the O-H, N-H and C-O groups. In pH analysis it is observed that on day 3 that ESP acidifies the incubation medium, and from day 7 onwards an alkalinization occurs, while COL acidifies the medium on day 3 and alkalizes the new walk near physiological pH again from day one. 14. Results were negative for both groups and are observed at a mass loss from incubation period to day 14. Results of histological analysis compared to the amount of days after formation of newly formed bone tissue and You can have a wide variety of components to store material waste. In the in vivo assays it was observed that in the different concentrations of ESP used, did not inhibit the cell proliferation of the used strains, showing no cytotoxic effects. Therefore, for future stages, genotocixity assays will be performed through the Comet and Micronucleus tests to evaluate DNA damage.
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    Efeito do scaffold de biosilicato®/espongina no reparo de defeitos em tíbias de ratos
    (Universidade Federal de São Paulo (UNIFESP), 2019-08-15) Fermino, Bianca Priscilla Dorileo [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Bone repair is a highly complex process that includes the interaction of a series of biological events to restore tissue integrity. Nevertheless, some situations may result in delayed consolidation and even nonunion (SENA et al., 2005; MARSELL; EINHORN, 2010). This scenario has required the development of new therapeutic practices, such as the manufacture of composites that unite the properties of two or more materials, mainly due to the possibility they offer to mimic bone tissue (SIQUEIRA; ZANOTTO, 2011). Thus, the combination of Biosilicate® (BS) (which would represent the inorganic part of bone tissue) with an organic component, such as spongin (ESP) from marine sponges, could represent a therapy with increased osteogenic potential. Given the above, the objective of the present study was to evaluate the effect of the Biosilicate® Spongine (BS/ESP) scaffold on the repair process of induced bone defects in rat tibias. In this study, 30 rats allocated to the following experimental groups were used: CG (control); BS; and BS/ESP. The animals were submitted to the surgical procedure to perform the tibial bone defect and received the respective materials. After 15 days, the animals were euthanized and the samples collected for histopathological, morphometric – where three variables were investigated: ratio of neoformed bone tissue volume (BV) to defect volume (TV), (BV/TV), %), values for the number of osteoblasts per tissue area (N.Ob/T.Ar, mm2) and representation for the osteoblast surface as a percentage of bone surface (Ob.S/BS, %) – and immunohistochemistry analysis, which was performed qualitatively and semiquantitatively for RankL (activator of nuclear factor kappa-B ligand) and Runx2 (runt-related transcription factor-2) factors. Histopathological analysis indicated the presence of granulation tissue and few inflammatory cells at the bone defect site for all groups. Furthermore, there was a prevalence of bone trabeculae in the periphery of the defect for the biomaterials groups and signs suggestive of BS and BS/ESP degradation in the center of the bone defect. Regarding the morphometric analysis, no statistically significant difference was observed in any of the analyzed variables. Regarding qualitative immunohistochemical analysis, RankL immunostaining for CG was predominant in granulation tissue, osteocytes and bone marrow. For BS and BS/ESP, this immunostaining was more evident in granulation tissue and newly formed bone. Immunostaining for Runx2, on the other hand, was observed in all experimental groups in granulation tissue and newly formed bone. And for quantitative immunohistochemical analysis no statistically significant differences were observed between the studied groups for RankL and Runx2. It was concluded that the BS and BS/ESP scaffolds allowed the growth of bone trabeculae mainly at the edges of the defect. In addition, both materials showed a positive immunostaining for RankL and Runx2, mainly in granulation tissue and newly formed bone.
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    Efeitos de scaffolds desenvolvidos a partir de compósitos bioativos extraídos de esponjas marinhas no processo de reparo ósseo de ratas osteoporóticas
    (Universidade Federal de São Paulo, 2023-12-20) Cruz, Matheus de Almeida [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Granito, Renata Neves [UNIFESP]; http://lattes.cnpq.br/1941145984734628; http://lattes.cnpq.br/7546924431469938; http://lattes.cnpq.br/7546924431469938; Universidade Federal de São Paulo (UNIFESP)
    Durante o processo de reparo ósseo, cerca de 5 a 20% dos casos podem culminar em um processo de consolidação anormal e resultar em um atraso na consolidação ou não união óssea. Uma vez este quadro instalado, pode gerar prejuízos na qualidade de vida destes indivíduos, altos índices de morbimortalidade e elevados custos ao sistema de saúde. Desta forma, se faz necessário a investigação de tratamentos que apresentem potencial osteogênico e que tenham a capacidade de acelerar o processo de reparo ósseo. Dentre estes recursos, pode-se evidenciar a aplicação de biomateriais, por exemplo, os materiais bioativos, como o biovidro (BG) e dos provenientes de esponjas marinhas como a biosilica (BS) e a espongina (SPG). Assim, o objetivo do presente estudo foi avaliar a biocompatibilidade dos compósitos de BS e BG associados ainda à SPG, por meio de estudo in vitro e in vivo, utilizando um modelo de defeito ósseo em tíbias de ratas osteoporóticas. Para o estudo in vitro, células MC3T3-E1. L929 e CHOK-1 foram analisadas através da viabilidade celular, ensaio de cometa, teste de micronúcleo e vermelho de alizarina. No estudo in vivo foram realizadas as seguintes análises: Histopatológica, histomorfométrica, Análise de colágeno e Imunohistoquímica. O estudo in vitro demostrou que, após 7 dias de cultura celular, houve um aumento significativo de viabilidade celular do grupo controle em comparação com os grupos BS, BS/SPG, BG e BG/SPG na concentração de 100%, nenhum dos materiais apresentou sinais de Genotoxicidade e os materiais apresentaram marcação por vermelho de alizarina. Para análise histológica, in vivo, 15 dias após a cirurgia, os grupos apresentaram tecido de granulação e algumas áreas de tecido ósseo neoformado. Trinta dias após a cirurgia, os grupos revelaram um tecido ósseo neoformado mais maduro e progressiva degradação do material. Todos os grupos tratados apresentaram deposição de colágeno organizado em rede após 30 dias. A análise de imuno-histoquímica demonstrou uma maior imunomarcação para Runx-2 em todos os grupos que receberam implantes de biomaterial, 15 e 30 dias após a cirurgia. Para a análise de OPG, observou-se um aumento da Imunomarcação para BS/SPG e BG/SPG em comparação com CG e BS e BG, 30 dias após a cirurgia. Desta forma, pode-se concluir que os estudos in vitro e in vivo apontaram que as amostras de BS e BS/SPG promoveram maior viabilidade celular e melhores propriedades biológicas nos defeitos ósseos na tíbia de ratas osteoporóticas, destacando o potencial dos compósitos associados a SPG para serem usados como enxertos ósseos em aplicações na medicina regenerativa.