Navegando por Palavras-chave "Estrogenetic Receptors"

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    Relação Entre Os Polimorfismos Pvuii E Xbai Do Gene Do Receptor De Estrogênio Alfa Com A Densidade Mamográfica Em Mulheres Após A Menopausa
    (Universidade Federal de São Paulo (UNIFESP), 2017-11-30) Baldisserotto, Fernanda Della Giustina [UNIFESP]; Nazario, Afonso Celso Pinto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Assess the relation between the presence of PVUII and XBAI polymorphisms in the estrogen receptor alpha gene and marrtmographic density in postmenopausal women. Methods For the present analysis, 189 postmenopausal women who had never used hormonal therapy and who did not have clinicai or mammographic features were selected. Based on the ACR-BIRAOS® 2003 classification, the mammographic density was determined by three independent readers (two subjective ratings and one computerized - Adobe Photoshop ® 7.0 software). Blood samples were available to extract DNA according to KIT GFX ® protocol. PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) was then used to identify the pc[ymorphisms. Results There was a high degree of agreement amoní; the trree readers to determine the mammographic density (Kappa>0.75). Sixty women (32%) had dense breasts and 129 (68%) had non-dense breasfci. The PVUII polymorphism was found in 132 (69.8%) of 189 women, while the XBAI was found in 135 (71.4%) of women. Parity (p=0.02) and body mass inaex (p<0.0001) were associated with mammographic density. It was observed that for the XBAI polymorphism, women with two mutated alleles were approximately 25 times more likely to be classified in dense breasts group (p=0.003) and the presence of both wild alleles was associated with fibroglandular tissue replacement by fat (p=0.02). Conclusions: There was no significant association of the PVLII polymorphism in the estrogen receptor alpha gene with mammograohic density (p=0.34). However, the XBAI polymorphism was observed at a higher mutated homozygous frequency in women with dense breasts and th are wau an increased frequency of wild-type homozygous and heterozygous vjomen with fat-replaced breasts (p=0.01).