Navegando por Palavras-chave "Ethylenethiourea"
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- ItemAcesso aberto (Open Access)Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-03-01) Lemos, Sidney Pereira Pinto [UNIFESP]; Martins, Jose Luiz [UNIFESP]; Lemos, Patrícia Veruska Ribeiro Barbosa; Silva, Silvio Romero Gonçalves e [UNIFESP]; Santos, Fernando Leandro dos; Silva Júnior, Valdemiro Amaro da; UNIVASF; Universidade Federal de São Paulo (UNIFESP); Federal Rural University Department of Veterinary Medicine; Federal Rural University Department of Animal Morphology and PhysiologyPURPOSE: The pathophysiology of abnormalities associated with myenteric plexus lesions remains imperfectly understood. Such abnormalities have been correlated with subocclusive intestinal conditions in children with Hirschsprung's disease, cases of chronic constipation and, postoperatively, in cases of anorectal anomalies. This study evaluated abnormalities of the myenteric plexus in fetus from female rats that received ethylenethiourea. METHODS: Female rats were exposed to ethylenethiourea on the 11th day of pregnancy (experimental group) or to 0.9% physiological solution (control group). Abnormalities were only found in the experimental group. The digestive tract muscle layer was analyzed morphometrically and changes to the frequencies of nerve plexus cells and interstitial cells of Cajal were evaluated, using hematoxylin-eosin, S-100 protein, neuron-specific enolase and C-Kit, respectively. RESULTS: Muscle and skeletal abnormalities were observed in 100%, anorectal anomalies in 86%, absent tail in 71%, short tail in 29%, duodenal atresia in 5%, esophageal atresia in 5% and persistent omphalomesenteric duct in 5%. Histopathological analysis showed a thinner muscle layer associated with lower frequencies of ganglion cells and interstitial cells of Cajal, in all gastrointestinal tract. CONCLUSION: Severe nerve plexus abnormalities associated with muscle layer atrophy were observed throughout the gastrointestinal tract in newborn rats exposed to ethylenethiourea.
- ItemAcesso aberto (Open Access)Análise morfológica do sistema digestório de recém-nascidos de ratas prenhes submetidas à ingestão de etilenotioureia(Universidade Federal de São Paulo (UNIFESP), 2010-10-27) Lemos, Sidney Pereira Pinto [UNIFESP]; Martins, Jose Luiz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)INTRODUCTION: Congenital abnormalities are important causes of death and severe sequelae, and improvement of the knowledge of chronic diseases such as aganglionosis and other abnormalities of the myenteric plexus is of great importance. OBJECTIVES: To morphologically, histologically and immunohistochemically evaluate abnormalities of the myenteric nervous system in the gastrointestinal tract of newborns from pregnant rats exposed to ethylenethiourea (ETU). METHOD: Newborns from female Wistar rats that had received ETU on the eleventh day of pregnancy were studied. Cesarean section was performed on the twentieth day of pregnancy. After birth, the newborns were analyzed and the congenital abnormalities were identified and recorded. Morphometry was performed on the muscle layer, and abnormalities in the ganglionic cells of the myenteric plexus and interstitial cells of Cajal (ICC) were evaluated, using HE, P S-100, C-KIT and neuron-specific enolase. The data obtained were expressed as means for each group. RESULTS: The newborns from the female rats exposed to ETU presented abnormalities of embryo formation, with musculoskeletal abnormalities in 100% of the newborns, anorectal abnormalities in 86%, taillessness in 71% and short or vestigial tail in 29%. The histopathological analysis showed atrophy of the muscle layer associated with lesions of myenteric plexus, in all segments of the gastrointestinal tract, characterized by reduced density in the ganglionic cells and ICC and thinning of the musculature, thus suggesting that the process occurred linearly. CONCLUSIONS: 1) ETU was efficient in producing various types of congenital abnormalities in the newborns from the exposed female rats. 2) ETU was efficient in producing severe abnormalities in the myenteric plexus of the gastrointestinal tract. 3) ETU promoted atrophy of the muscle layer throughout the gastrointestinal tract. 4) The nerve abnormalities in the myenteric plexus and muscle abnormalities occurred concomitantly throughout the gastrointestinal tract.
- ItemSomente MetadadadosEffect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats(Acta cirurgica brasileira, 2016) Fiorindo Faria, Danilo Jose [UNIFESP]; Simoes, Manuel de Jesus [UNIFESP]; Teixeira, Luciana Cristina [UNIFESP]; Pinheiro Faria, Angela Tucci; Simoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP]; Martins, Jose Luiz [UNIFESP]PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADR
- ItemAcesso aberto (Open Access)Evaluation of an experimental model for anorectal anomalies induced by ethylenethiourea(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2007-04-01) Macedo, Mauricio [UNIFESP]; Martins, José Luiz [UNIFESP]; Meyer, Karine Furtado [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To evaluate an experimental model for anorectal anomalies and their principal associated malformations induced by ethylene thiourea (ETU). METHODS: Rat fetuses were utilized, divided into two groups: experimental group - fetuses from rats that received ETU on the 11th day of gestation at the dose of 125 mg/kg, diluted in distilled water to 1% concentration (12.5 ml/kg); and control group - fetuses from rats that received distilled water alone, at a volume of 12.5 ml/kg. On the 21st day of gestation, the animals were sacrificed by hypoxia in a carbon dioxide chamber, followed by laparotomy to remove the fetuses. These were initially examined externally to determine the sex and whether anorectal anomalies and malformations of the vertebral column and tail were present. Then, with the aid of microscopy, the fetuses underwent exploratory laparotomy to characterize the type of anorectal anomaly and investigate urological malformations. RESULTS: None of the fetuses in the control group presented anorectal anomaly, vertebral column malformation or urological structural alterations. In the experimental group, 71% presented anorectal anomaly, 80% presented vertebral column alterations and 35% presented urological alterations. CONCLUSION: The model described was shown to be easy to implement and presented results that allow its use in studying anorectal anomalies and associated malformations.
- ItemAcesso aberto (Open Access)Hepatic damage in newborns from female rats exposed to the pesticide derivative ethylenethiourea(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-12-01) Lemos, Patrícia Veruska Ribeiro Barbosa; Martins, Jose Luiz [UNIFESP]; Lemos, Sidney Pereira Pinto [UNIFESP]; Santos, Fernando Leandro Dos; Silva, Sílvio Romero Gonçalves E; Sao Francisco Valley Federal University Department of Medicine; Universidade Federal de São Paulo (UNIFESP); UNIVASF Department of Medicine; Federal Rural University Department of Veterinary MedicinePURPOSE: To evaluate hepatic morphological-histological abnormalities in newborns from female rats exposed to ethylenethiourea. METHODS: A randomized study was conducted on fifty-five newborn Wistar rats were studied: 34 in the experimental group, whose mothers had been exposed to 1% ethylenethiourea; and 21 in the control group, whose mothers had received 0.9% physiological solution. The solution was administered via gavage on the 11th day of gestation. Cesarean section was performed on the 20th day of gestation. The newborns' livers were examined and any morphological-histological abnormalities were registered. The presence of megakaryocytes was quantified in 50 microscope fields, as the total number of these cells per mm². RESULTS: The entire experimental group presented abnormalities of embryonic formation, with musculoskeletal anomalies, digestive system anomalies, hepatic congestion and friability, hydrops and delayed intrauterine growth. The histopathological analysis showed that morphological-histological hepatic destructuring had occurred in all entire experimental with removal of the hepatic trabeculae and severe hepatic megakaryocytosis. The mean megakaryocyte density ranged from 107.9 to 114.2 per mm², and it was eight times greater than in the control group, thus characterizing a situation of extramedullary hematopoiesis. CONCLUSION: The fetal exposure to ethylenethiourea caused hepatic damage characterized by severe extramedullary hematopoiesis.
- ItemAcesso aberto (Open Access)Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2007-12-01) Macedo, Mauricio [UNIFESP]; Martins, José Luiz [UNIFESP]; Meyer, Karine Furtado [UNIFESP]; Soares, Iberê Cauduro; Universidade Federal de São Paulo (UNIFESP); Hospital of São Paulo (HSPE) Public ServantsPURPOSE: To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.