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- ItemAcesso aberto (Open Access)A aplicação de adesivo de cianoacrilato por histeroscopia e os testes de perviedade na esterilização tubária de ovelhas(Colégio Brasileiro de Cirurgiões, 2008-02-01) Bigolin, Sergio [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Rivoire, Henri Chaplin; Simões, Ricardo Santos [UNIFESP]; Fagundes, Anna Tereza Negrini; Simões, Manuel de Jesus [UNIFESP]; Universidade Regional Integrada do Alto Uruguai e Missões Campus Erechim Assistente da Disciplina de Embriologia; Universidade Federal de São Paulo (UNIFESP); Fundação Universidade do Rio Grande Departamento de Cirurgia; Universidade de Santo AmaroBACKGROUND: To evaluate, through pregnancy and pressure burst tests, the effectiveness of sheep uterine tube sterilization through hysteroscopic insertion of cyanoacrylate adhesive. METHODS: Adult sheep (n=12), with one previous pregnancy, were distributed as follows: PO Group (n=3) submitted to laparotomy and Pomeroy surgical procedure of tubal obstruction, Group SH (n=3) submitted to hysteroscopy application of saline solution inside the tubal isthmus, and Group AD (n=6) submitted to hysteroscopy application of 0.5 mL of n-2-butil-cyanoacrylate inside the tubal isthmus. They were allowed to copulate with fertile males during ninety days. The non pregnant sheep, at the 90th day, were submitted to laparotomy, the uterine tubes were removed and their diameters analyzed. The uterine tubes were evaluated for patency with methylene blue dye injection. The overpressure burst test (80mmHg) was then applied through the uterine cervix. Data were evaluated by t Student and Fisher test. RESULTS: All sheep from groups PO and AD did not get pregnant (0%) in contrast with sheep from group SH (100%); the adhesive remained stable inside the tubal lumen. The diameter of the proximal tube in AD group (0.650 ± 0.16cm) was significantly wider than the PO group (0.195 ± 0.04* cm) (p= 0.01). The overpressure burst and dye patency tests were negative for all animals from PO group and only one animal in AD group was positive for the pressure burst test. CONCLUSION: The hysteroscopic insertion of cyanoacrylate inside the tubal lumen of the sheep was effective to obstruct the uterine tube and to promote the sterilization, and would be an option for future applying in human beings.
- ItemAcesso aberto (Open Access)Esterilização tubária com adesivo cirúrgico sintético: estudo experimental(Colégio Brasileiro de Cirurgiões, 2003-10-01) Rivoire, Henri Chaplin; Fagundes, Djalma José [UNIFESP]; Taha, Murched Omar [UNIFESP]; Novo, Neil Ferreira [UNIFESP]; Juliano, Yara [UNIFESP]; FURG-RS Departamento de Cirurgia; Universidade Federal de São Paulo (UNIFESP)BACKGROUND: Study of the patency of the uterine tube of rabbits submitted to the transvaginal approach application of n-butyl-2-cyanoacrylate. METHODS: Twenty rabbits were used (Orictolagus cuniculus) of the lineage New Zealand, distributed in two groups, 1 (control) and 2 (try). Control group was submitted to the technique for sterilization through the method proposed by Pomeroy and the group experiment had the application of 0,25mL of the surgical adhesive n-butyl-2-cyanoacrylate in the isthmus of uterine tube, via transvaginal. After four weeks and four time mated and the clinical diagnosis of gestation appearance, they were submitted to three patency tests: histerosalpingography (in vivo), patency test with color metilen blue (in vitro) and test of breaking pressure (in vitro). Digital computerized morfometry was accomplished to measure the diameter of tubes, mucous membrane and miosalpinx. RESULTS: The results showed leak in only one uterine tube of the group 2, although the sticker was present in the isthmus of uterine tube. Statistical significancy was shown by the morfometry that showed increase in the measures of the diameter tube, of mucous membrane and of miosalpinx, that we considered to be for the presence of the polymeric formed by the adhesive, not cellular damages. CONCLUSIONS: We concluded that the application n-butyl-2-cyanoacrylate of transvaginal route in the uterine tubes of rabbits is so effective for sterilization as the method of Pomeroy.