Navegando por Palavras-chave "Fator De Crescimento Transformador Beta"

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    Alterações da expressão de genes de matriz extracelular e da familia TGFB nas lesões do manguito rotator
    (Universidade Federal de São Paulo (UNIFESP), 2019-03-29) Figueiredo, Eduardo Antonio De [UNIFESP]; Cohen, Moises [UNIFESP]; Ejnisman, Benno; http://lattes.cnpq.br/1124807952912223; http://lattes.cnpq.br/6174355233304675; http://lattes.cnpq.br/2837820077224310; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Lack of synthesis of extracellular matrix compounds may contribute to degeneration of the tendons. Objective: we aimed to evaluate the expression of extracellular matrix and TGFB family members in ruptured and non-ruptured tendons of the rotator cuff, as well as the effect of clinical factors on gene expression in tendon samples, and the relationship between histological findings and altered gene expression. Methods: Injured and non-injured supraspinatus tendon samples and subscapular non-injured tendon samples were collected from 38 patients with rotator cuff tears. Noninjured supraspinatus tendons were obtained from eight controls. Specimens were used for histological evaluation, quantification of collagen fibers, and mRNA and protein expression analyses. Results: Increased COL1A1, COL1A2, COL3A1, COL5A1, FN1, TNC, and TGFBR1 mRNA expression was observed in the tear samples (p < 0.05). Duration of symptoms was correlated with the levels of collagen type I/III fibers (p = 0.032; p = 0.0447) and FN1 immunostaining (p = 0.031; p = 0.417). Smoking was associated with increased frequency of microcysts, myxoid degeneration, and COL5A1, FN1, TNC, and TGFB1 mRNA expression (p < 0.05). FN1 immunostaining was correlated with the number of years of smoking (p=0.048; p=0.384). Lower levels of collagen type I/III fibers were detected in samples with fissures (p=0.046). High frequency of microcysts was associated with increased COL5A1, FN1, and TNC expression (p < 0.05, for all comparisons). Neovascularization was associated with reduced FN1 (p=0.035) and TGFBR1 expression (p=0.034). Conclusion: Our findings show differential expression of matrix extracellular genes and TGFB family members in the degeneration process involved in rotator cuff tears. These molecular alterations are influenced by clinical factors.
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    Expressão proteica de BMP-7, MMP-9 e TGF-β e suas correlações com as interleucinas 6 e 10 nas rinossinusites crônicas
    (Universidade Federal de São Paulo (UNIFESP), 2020-08-27) Lucas, Bernardo Relvas [UNIFESP]; Pezato, Rogerio [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Chronic Rhinosinusitis has a worldwide prevalence in the general population of 5% to 12% and is divided phenotypically into two main groups: Chronic Rhinosinusitis without Nasal Polyposis and Chronic Rhinosinusitis with Nasal Polyposis, presenting processes distinct remodeling. Involved in the remodeling process, the BMP-7, MMP-9 and TGF-β proteins appear to exhibit different behaviors in the two phenotypes and different relationships with the interleukins IL-6 and IL-10, which will be investigated in this study. Objective: to evaluate the expression of BMP-7, MMP-9, TGF-β protein in Rhinosinusitis with and without Nasal Polyposis and its relationship with interleukins IL-6 and IL-10. Method: the sample consisted of 86 participants, divided into three groups: one of patients with Chronic Rhinosinusitis With Nasal Polyposis (n = 34), one of patients with Chronic Rhinosinusitis without Nasal Polyposis (n= 26), both groups according to EPOS 2012 criteria, and a control group without inflammatory nasal mucosa disease (n = 26). BMP-7, MMP-9, TGF-β, IL-6 and IL-10 were dosed into the tissue by Elisa method. The presence of eosinophils was evaluated by immunohistochemical Hematoxylin/ Eosin. Results: the high-power field (HPF) eosinophil count in the Nasal Polyp sample was above 5 eosinophils per high-power field in 91% of the cases and 73% presented more than 10 eosinophils by High-Power Field. TGF-β level were significantly lower in the polypoid tissue group when compared to control group and Chronic Rhinosinusitis without Nasal Polyposis group. Dosage of IL-6 was significantly higher in the Nasal Polyps group when compared to the other groups. Dosage of IL-10 was significantly higher in control group when compared to the other groups. There were significant negative correlations between IL-6 and IL-10 in all group, and between MMP-9 and IL-6 in control group and Nasal Polyp group. There was a positive correlation between MMP-9 and IL-10 in Nasal Polyp group. There was a positive correlation between BMP-7 and IL-10 in all groups. Finally, the correlation between TGF-β and IL-6 was positive in control group and negative in Chronic Rhinosinusitis without Nasal Polyposis group. Conclusion: In Chronic Rhinosinusitis without Nasal Polyposis there is a low concentration of IL-10 and a high concentration of TGF-β. In Nasal Polyposis there is a low concentration of TGF-β and IL-10 and an increase in IL-6. IL-6 and IL-10 have a negative correlation with each other and distinct interactions with BMP-7 and MMP-9 in the groups analyzed. We concluded, therefore, that there is a close relationship between interleukins 6 and 10 and the main proteins involved in the remodeling process in Chronic Rhinosinusitis.