Navegando por Palavras-chave "Feline"

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    Feline sporotrichosis due to Sporothrix brasiliensis: an emerging animal infection in São Paulo, Brazil
    (Biomed Central Ltd, 2014-11-19) Montenegro, Hildebrando; Rodrigues, Anderson Messias [UNIFESP]; Dias, Maria Adelaide Galvao; Silva, Elisabete Aparecida da; Bernardi, Fernanda; Camargo, Zoilo Pires de [UNIFESP]; Zoonosis Control Ctr São Paulo; Universidade Federal de São Paulo (UNIFESP)
    Background: Sporotrichosis is a mycotic infectious disease that is generally acquired by traumatic inoculation of contaminated materials especially from plant debris or through bites and scratches from diseased animals, such as domestic cats. It affects the skin, lymphatic system, and other organs in the warm-blooded host. Etiological agents are embedded in the plant-associated order Ophiostomatales. With essential differences between possible outbreak sources and ecological niche, host-environment interactions are classic determinants of risk factors for disease acquisition. Sporotrichosis outbreaks with zoonotic transmission, such as those that are ongoing in southern and southeastern Brazil, have highlighted the threat of cross-species pathogen transmission. Sporothrix brasiliensis has emerged as a human threat owing to the intimate contact pattern between diseased cats and humans in endemic areas.Results: We describe the recent emergence of feline sporotrichosis in the metropolitan region of São Paulo, Brazil, with an overwhelming occurrence of S. brasiliensis as the etiological agent. A phylogenetic and a haplotype approach were used to investigate the origin of this epidemic and the impact of feline transmission on genetic diversity. During the last 3-year period, 163 cases of feline sporotrichosis were reported in São Paulo with proven S. brasiliensis culture. the haplotype diversity of feline S. brasiliensis isolates revealed the expansion of a clonal population with low genetic diversity. Haplotype analysis confirmed that isolates from São Paulo shared the haplotype originated in the long-lasting outbreak of cat-transmitted sporotrichosis in Rio de Janeiro, which differed from the haplotype circulating in the Rio Grande do Sul epidemic.Conclusions: the fast spread of sporotrichosis in a short period of time highlights the potential for outbreaks and suggests that the mycosis may affect an urban population with a high concentration of susceptible felines. the feline sporotrichosis epidemic shows no signs of slowing, and this epidemiological pattern may require specific public health strategies to control future outbreaks.
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    Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigens
    (Elsevier B.V., 2011-01-27) Fernandes, Geisa Ferreira [UNIFESP]; Lopes-Bezerra, Leila Maria; Bernardes-Engemann, Andrea Reis; Pacheco Schubach, Tania Maria; Galvao Dias, Maria Adelaide; Pereira, Sandro Antonio; Camargo, Zoilo Pires de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade do Estado do Rio de Janeiro (UERJ); Fiocruz MS; Ctr Zoonosis Prefeitura São Paulo
    The main objective of this study is to standardize an ELISA for the diagnosis of feline sporotrichosis. Sporothrix schenckii is the etiological agent of human and animal sporotrichosis. Cats may act as reservoirs for S. schenckii and can transmit the infection to humans by a bite or scratch. There are few methods for the serological diagnosis of fungal diseases in animals. in this paper, an ELISA test for the diagnosis of cat sporotrichosis is proposed, which detects S. schenckii-specific antibodies in feline sera. Two different kinds of antigens were used: SsCBF, a specific molecule from S. schenckii that consists of a Con A-binding fraction derived from a peptido-rhamnomannan component of the cell wall, and a S. schenckii crude exoantigen preparation. the ELISA was developed, optimized, and evaluated using sera from 30 cats with proven sporotrichosis (by culture isolation); 22 sera from healthy feral cats from a zoonosis center were used as negative controls. SsCBF showed 90% sensitivity and 96% specificity in ELISA; while crude exoantigens demonstrated 96% sensitivity and 98% specificity. the ELISA assay described here would be a valuable screening tool for the detection of specifics. schenckii antibodies in cats with sporotrichosis. the assay is inexpensive, quick to perform, easy to interpret, and permits the diagnosis of feline sporotrichosis. (C) 2010 Elsevier B.V. All rights reserved.
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    Serological Survey of Paracoccidioidomycosis in Cats
    (Springer, 2013-10-01) Oliveira, Gabriela Goncalves de; Belitardo, Donizeti Rodrigues; Stipp Balarin, Mara Regina; Freire, Roberta Lemos; Camargo, Zoilo Pires de [UNIFESP]; Ono, Mario Augusto; Universidade Estadual de Londrina (UEL); Universidade Federal de São Paulo (UNIFESP)
    The objective of the present study was to evaluate infection of cats by Paracoccidioides brasiliensis. Serum samples of 136 cats from rural (n = 86) and urban areas (n = 50) were analyzed by indirect ELISA and immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens, respectively, and an overall reactivity of 31.6 % was observed by ELISA although no reactivity was detected by immunodiffusion. the positivity observed in animals living in rural areas (48.8 %) with free access to soil was significantly higher (P < 0.0001) than among urban animals (2 %) with limited access to soil, although no significant difference was observed in relation to age or sex. the high rates of positivity observed in cats from rural areas suggest that not diagnosed cases of this mycosis may be occurring in cats living in endemic areas for human paracoccidioidomycosis. This is the first report showing serological evidence of P. brasiliensis infection in cats.