Navegando por Palavras-chave "Fos Immunoreactivity"

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    Efeito de concentrações suprafisiológicas de hormônios esteroides gonadais na resposta de estresse em ratas
    (Universidade Federal de São Paulo (UNIFESP), 2018-02-08) Mariotti, Flora Franca Nogueira [UNIFESP]; Le Sueur-Maluf, Luciana [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    It Is Estimated That Infertility Affects About 10-15% Of Couples Of Childbearing Age Within The World Population, While Annually 7-10% Of Couples Of Reproductive Age In Brazil Rely On Services Specialized In Fertility Disorders Treatment. From A Psychosocial Perspective, Infertile Individuals Experience Intense Psychological Stress, Related To Emotional Disorders, Which Have Repercussions On Marital And Social Relationships. While The Stress Impact On Assisted Reproduction Treatment (Art) Success Has Been Comprehensively Studied, The Role Of Supraphysiological Concentrations Of Gonadal Hormones (Commonly Experienced By Women In Treatment) On Stress Response, Remains To Be Elucidated. The Aim Of This Study Was To Evaluate The Effect Of Supraphysiological Levels Of Gonadal Hormones On The Induced-Stress Response In Female Wistar Rats. In Order To Mimic The Context Of Art In Rodents, Female Rats (11 Weeks Old) With Proven Regular Cycle Were Submitted To The Superovulation Protocol (150 Ui/Kg Ip Of Pregnant Mare
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    Unpredictable chronic mild stress exerts anxiogenic-like effects and activates neurons in the dorsal and caudal region and in the lateral wings of the dorsal raphe nucleus
    (Biomed Central Ltd, 2016) Lopes, Danielle Abreu [UNIFESP]; Lemes, Jessica Alves [UNIFESP]; Melo-Thomas, Liana; Schor, Herbert [UNIFESP]; Andrade, José Simões de [UNIFESP]; Machado, Carla M.; Horta-Junior, Jose A. C.; Céspedes, Isabel Cristina [UNIFESP]; Viana, Milena de Barros [UNIFESP]
    In previous studies, we verified that exposure to unpredictable chronic mild stress (UCMS) facilitates avoidance responses in the elevated T-maze (ETM) and increased Fos-immunoreactivity in different brain structures involved in the regulation of anxiety, including the dorsal raphe (DR). Since, it has been shown that the DR is composed of distinct subpopulations of serotonergic and non-serotonergic neurons, the present study investigated the pattern of activation of these different subnuclei of the region in response to this stress protocol. Male Wistar rats were either unstressed or exposed to the UCMS procedure for two weeks and, subsequently, analyzed for Fos-immunoreactivity (Fos-ir) in serotonergic cells of the DR. To verify if the anxiogenic effects observed in the ETM could be generalized to other anxiety models, a group of animals was also tested in the light/dark transition test after UCMS exposure. Results showed that the UCMS procedure decreased the number of transitions and increased the number of stretched attend postures in the model, an anxiogenic effect. UCMS exposure also increased Fos-ir and the number of double-labeled neurons in the mid-rostral subdivision of the dorsal part of the DR and in the mid-caudal region of the lateral wings. In the caudal region of the DR there was a significant increase in the number of Fos-ir. No significant effects were found in the other DR subnuclei. These results corroborate the idea that neurons of specific subnuclei of the DR regulate anxiety responses and are differently activated by chronic stress exposure. (C) 2015 Elsevier B.V. All rights reserved.