Navegando por Palavras-chave "Fosfatase Calcineurina"

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    Proteômica Aplicada Ao Estudo De Corpúsculos Lipídicos E Sua Relação Com A Atividade Da Fosfatase Calcineurina Do Fungo Paracoccidioides Brasiliensis
    (Universidade Federal de São Paulo (UNIFESP), 2018-11-05) Lima, Beatriz Pereira De Sousa [UNIFESP]; Campos, Claudia Barbosa Ladeira De [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To Investigate The Role Of Calcineurin In Lipid Metabolism, Especially Lipid Droplets In Yeasts Of Paracoccidioides Brasiliensis. Method: Triplicate Samples Of Total Yeast Proteins Grown At 0.2% And 2% Glucose In The Absence And Presence Of Cyclosporin A Were Analyzed By Lc-Ms / Ms Type Mass Spectrometry. The Intensities Of The Proteins Identified By The Mass Spectrometer Were Submitted To The Triplicate Homogeneity Test. Working With Three Biologically Comparable Groups (1) 0.2% Control Versus 0.2% Csa, (2) 0.2% Control Versus 2% Control And (3) 2% Control Versus 2% Csa, Differentially Expressed Proteins Were Determined For Each Sample Combination Considering Pvalor " 0.05 And Log2 (Fold Change)> 1 And <-1. The Exclusive And Differentially Expressed Proteins Of Each Of The Three Comparable Groups Were Analyzed For The Biological Pathways That Are Involved From The Use Of The Keeg Pathway Virtual Tool. Results: Yeasts Grown At 2% Of Glucose With Csa Present A Metabolic Profile That Indicates To Be