Navegando por Palavras-chave "Fucan"
Agora exibindo 1 - 2 de 2
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)Heterofucans from Dictyota menstrualis have anticoagulant activity(Associação Brasileira de Divulgação Científica, 2004-02-01) Albuquerque, Ivan Rui Lopes; Queiroz, Karla Cristiana Souza; Alves, Luciana Guimarães [UNIFESP]; Santos, Elizeu Antunes dos [UNIFESP]; Leite, Edda Lisboa [UNIFESP]; Rocha, Hugo Alexandre Oliveira [UNIFESP]; Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais; Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia; Universidade Federal de São Paulo (UNIFESP)Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all fractions are composed mainly of fucose, xylose, galactose, uronic acid, and sulfate. The anticoagulant activity of these heterofucans was determined by activated partial thromboplastin time (APTT) using citrate normal human plasma. Only the fucans F1.0v and F1.5v showed anticoagulant activity. To prolong the coagulation time to double the baseline value in the APTT, the required concentration of fucan F1.0v (20 µg/ml) was only 4.88-fold higher than that of the low molecular weight heparin Clexane® (4.1 µg/ml), whereas 80 µg/ml fucan 1.5 was needed to obtain the same effect. For both fucans this effect was abolished by desulfation. These polymers are composed of fucose, xylose, uronic acid, galactose, and sulfate at molar ratios of 1.0:0.8:0.7:0.8:0.4 and 1.0:0.3:0.4:1.5:1.3, respectively. This is the fist report indicating the presence of a heterofucan with higher anticoagulant activity from brown seaweed.
- ItemAcesso aberto (Open Access)Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora(Associação Brasileira de Divulgação Científica, 2005-04-01) Silva, T.m.a.; Alves, L.g. [UNIFESP]; Queiroz, K.c.s. de; Santos, M.g.l.; Marques, C.t.; Chavante, Suely Ferreira [UNIFESP]; Rocha, Hugo Alexandre Oliveira [UNIFESP]; Leite, E.l.; Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia; Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais; Universidade Federal de São Paulo (UNIFESP)The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.