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    Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
    (Sociedade Brasileira de Pesquisa Odontológica - SBPqO, 2007-06-01) Nunes, Fábio Daumas; Valenzuela, Maria Da Graça Silva; Rodini, Camila Oliveira; Massironi, Silvia Maria Gomes; Ko, Gui Mi [UNIFESP]; University of São Paulo School of Dentistry Department of Oral Pathology; University of São Paulo Biomedical Sciences Institute Department of Immunology; Universidade Federal de São Paulo (UNIFESP)
    A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh.