Navegando por Palavras-chave "Glycine"
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- ItemAcesso aberto (Open Access)Efeitos da glicina na mucosite oral induzida por 5-fluorouracil em hamster(Universidade Federal de São Paulo (UNIFESP), 2010-07-28) Sá, Odara Maria de Sousa [UNIFESP]; Caran, Eliana Maria Monteiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Mucosite oral é complicação comum no tratamento do câncer. A glicina demonstra efeito antiinflamatório, imunomodulador e citoprotetor. Este estudo tem como objetivo avaliar os efeitos da suplementação de glicina na reparação da mucosite oral induzida por 5-fluorouracil em hamster. Os animais foram divididos em dois grupos: grupo experimental (GI; n=20) e grupo controle (GII; n=20) ambos receberam injeção intraperitoneal de 5-fluorouracil no 1° e 3° dia. Os animais tiveram a sua bolsa jugal direita evertida e arranhada superficialmente no dia 3. O Grupo I, foi submetido ao tratamento com glicina a 5% por infusão intraperitoneal durante 7 dias e o Grupo II, recebeu placebo. A mucosa do GI e GII foi avaliada clinicamente, por meio de escore, no D3 e D7. Ao final do experimento a bolsa jugal dos animais de ambos os grupos foi retirada e avaliada segundo parâmetros histopatológicos e bioquímicos. Os grupos I e II apresentaram acentuado processo inflamatório durante o período inicial, segundo a avaliação clínica. No GI houve redução da severidade da mucosite, diminuição do processo inflamatório, cicatrização acelerada e redução da peroxidação lipídica quando comparado ao GII no final do experimento (p < 0,001). A suplementação com glicina demonstrou ser promissor instrumento para tratamento da mucosite, devido aos seus efeitos no processo inflamatório. Palavras chaves: Glicina; Estomatite; Fluoruracila; Cricetulus.
- ItemAcesso aberto (Open Access)Efeitos da glicina nas expressões imunohistoquimicas de PDGF, FGF e EGF em mucosite oral induzita em hamster(Universidade Federal de São Paulo (UNIFESP), 2017-12-08) Sá, Odara Maria de Sousa [UNIFESP]; Caran, Eliana Maria Monteiro [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Lopes, Nilza Nelly Fontana [UNIFESP]; Maria Teresa de Seixas Alves : http://lattes.cnpq.br/0357765137541523; Nilza Nelly Fontana Lopes : http://lattes.cnpq.br/8672330651019555; http://lattes.cnpq.br/1783139918188371; http://lattes.cnpq.br/3916790331038294; Universidade Federal de São Paulo (UNIFESP)Introduction: Oral mucositis is one of the most frequent toxic effects of chemotherapeutic and/or radiotherapeutic treatment, resulting from complex multifaceted biological events involving DNA damage, multiple signaling, and interactions between epithelial, submucosal, and connective tissue. Clinical manifestations such as pain, difficulty in swallowing and communication, and infections have a negative impact on the tolerance to treatment and the quality of life of cancer patients. Preventive measures and curative treatment of mucositis, secondary to chemotherapy and radiotherapy, are still not well established, requiring cooperative studies and new synchronic and effective therapeutic approaches. On the other hand, the nonessential amino acid glycine has anti-inflammatory, immunomodulatory and cytoprotective action, being a potential therapeutic option in mucositis. Therefore, we performed this study with the objective of evaluating the effects of glycine on the expression of collagen and growth factors, platelet (PDGF) and epidermal (EGF), in experimental models of oral mucositis, as apoptotic markers. Methods: The biological material used was the hamster jugal mucosa. The mucositis of which was induced by the protocol proposed by Sonis. A total of 40 hamsters were used, divided into two groups: group I (n= 20)- control; Group II (n= 20) supplemented with 5% intraperitoneal glycine, 0,2 ml (2,0 mg /g) diluted in herpes . On day 7 histopathological sections were performed in order to perform the immune-histochemical method, the evaluation of quantitative and qualitative collagen expression by means of picrossirius under polarized light and the growth factors: EGF and PDGF. Results: It was observed that the group supplemented with glycine higher amounts of collagen expression and predominance type of collagen I when compared to the compared to the control group (p≤0.0002). The glycine group presented lower immunoexpression (p≤0,0001) of the growth factors, EGF and PDGF in relation to the control group. The animals that expressed type I collagen fibers, independent of the experimental group, presented higher amount of collagen and less immunoexpression of the growth factors: EGF and PDGF. Conclusion: The group supplemented with glycine showed a marked healing process of the oral mucosite, demonstrated by the predominance of collagen type I and reduction of growth factors, EGF and PDGF.
- ItemAcesso aberto (Open Access)Glycine reduces tissue lipid peroxidation in hypoxia-reoxygenation-induced necrotizing enterocolitis in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2006-06-01) Meyer, Karine Furtado [UNIFESP]; Martins, José Luiz [UNIFESP]; Freitas Filho, Luiz Gonzaga de [UNIFESP]; Oliva, Maria Luiza Vilela [UNIFESP]; Patricio, Francy Reis da Silva [UNIFESP]; Macedo, Mauricio [UNIFESP]; Wang, Lina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Public Servant Hospital of São PauloPURPOSE: To assess the protective effect of glycine in an experimental model of Neonatal Necrotizing Enterocolitis (NEC). METHODS: Fifty (50) neonatal Wistar rats, from a litter of six female rats and weighing 4 to 6 grams, were used. Five animals were cannibalized and the 45 remaining were distributed into three groups: the G1 normal control group (n=12); the G2 Group (n=16), of animals that underwent hypoxia-reoxygenation (HR); the G3 Group of animals (n=17) that underwent HR following a 5% intraperitoneal glycine infusion. The animals underwent hypoxia in a CO2 chamber receiving an air flow of 100% CO2 for 5 minutes and reoxygenation receiving an O2 flow at 100% for 5 minutes. One centimeter long small bowel and colon segments were prepared for histological analysis. The rest of the bowel was removed in a block and frozen at minus 80°C for homogenization and determination of tissue malondialdehyde (MDA). Tissue lesions were classified as Grade 0 to Grade 5, according to the level of damaged mucosa. RESULTS: The animals in Group G1 had levels of small bowel and colon lesion significantly smaller as compared to the animals in Groups G2 and G3. The G2 group had mean MDA values significantly higher than the animals in the G1 (p = .015) and G3 (p=0.021) groups. MDA values did not differ significantly (p = 0.992) for the animals in groups G1 and G3. CONCLUSION: Glycine reduces tissue MDA levels (a measurement of lipid peroxidation) following HR in neonatal rats.