Navegando por Palavras-chave "Growth factors"
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- ItemSomente MetadadadosAngiogenesis and growth factor modulation induced by altelnagin C, a snake venom disintegrin-like, cysteine-rich protein on a rat skin wound model(Elsevier B.V., 2008-11-01) Correia St Ana, Estela Maria; Cacao Paiva Gouvea, Cibele Marli; Nakaie, Clovis Ryuichi [UNIFESP]; Selistre-de-Araujo, Heloisa Sobreiro; Universidade Federal de São Carlos (UFSCar); Univ Fed Alfenas; Universidade Federal de São Paulo (UNIFESP)In this work, we show that alternagin-C (ALT-C) and ALT-C PEP, a peptide derived from its sequence, were able to induce angiogenesis ill Wounded rat skin. A spherical Cutaneous excision was made in the back of each animal and treated with three different concentrations of ALT-C or ALT-C PEP. After that, the skin was removed and analyzed to verify the presence of new vessels and the expression of growth factors. ALT-C and ALT-C PEP induced the formation of new vessels and modulated the expression of growth factors, mainly VEGF and FGH. the expression of VEGF increased and it Could be detected Up to 7 days after injury. FGH also significantly increased, but at a lesser extent than VEGF. in conclusion, the present Study shows for the first time the stimulation of angiogenesis in an injured tissue by a disintegrin-like protein and that ALT-C may exert this effect by modulating the expression of growth factors.(c) 2008 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)Efeitos da glicina nas expressões imunohistoquimicas de PDGF, FGF e EGF em mucosite oral induzita em hamster(Universidade Federal de São Paulo (UNIFESP), 2017-12-08) Sá, Odara Maria de Sousa [UNIFESP]; Caran, Eliana Maria Monteiro [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Lopes, Nilza Nelly Fontana [UNIFESP]; Maria Teresa de Seixas Alves : http://lattes.cnpq.br/0357765137541523; Nilza Nelly Fontana Lopes : http://lattes.cnpq.br/8672330651019555; http://lattes.cnpq.br/1783139918188371; http://lattes.cnpq.br/3916790331038294; Universidade Federal de São Paulo (UNIFESP)Introduction: Oral mucositis is one of the most frequent toxic effects of chemotherapeutic and/or radiotherapeutic treatment, resulting from complex multifaceted biological events involving DNA damage, multiple signaling, and interactions between epithelial, submucosal, and connective tissue. Clinical manifestations such as pain, difficulty in swallowing and communication, and infections have a negative impact on the tolerance to treatment and the quality of life of cancer patients. Preventive measures and curative treatment of mucositis, secondary to chemotherapy and radiotherapy, are still not well established, requiring cooperative studies and new synchronic and effective therapeutic approaches. On the other hand, the nonessential amino acid glycine has anti-inflammatory, immunomodulatory and cytoprotective action, being a potential therapeutic option in mucositis. Therefore, we performed this study with the objective of evaluating the effects of glycine on the expression of collagen and growth factors, platelet (PDGF) and epidermal (EGF), in experimental models of oral mucositis, as apoptotic markers. Methods: The biological material used was the hamster jugal mucosa. The mucositis of which was induced by the protocol proposed by Sonis. A total of 40 hamsters were used, divided into two groups: group I (n= 20)- control; Group II (n= 20) supplemented with 5% intraperitoneal glycine, 0,2 ml (2,0 mg /g) diluted in herpes . On day 7 histopathological sections were performed in order to perform the immune-histochemical method, the evaluation of quantitative and qualitative collagen expression by means of picrossirius under polarized light and the growth factors: EGF and PDGF. Results: It was observed that the group supplemented with glycine higher amounts of collagen expression and predominance type of collagen I when compared to the compared to the control group (p≤0.0002). The glycine group presented lower immunoexpression (p≤0,0001) of the growth factors, EGF and PDGF in relation to the control group. The animals that expressed type I collagen fibers, independent of the experimental group, presented higher amount of collagen and less immunoexpression of the growth factors: EGF and PDGF. Conclusion: The group supplemented with glycine showed a marked healing process of the oral mucosite, demonstrated by the predominance of collagen type I and reduction of growth factors, EGF and PDGF.
- ItemSomente MetadadadosGlycosaminoglycans from aged human hippocampus have altered capacities to regulate trophic factors activities but not A beta 42 peptide toxicity(Elsevier B.V., 2012-05-01) Minh Bao Huynh; Villares, Joao [UNIFESP]; Diaz, Julia Elisa Sepulveda; Christiaans, Stephy; Carpentier, Gilles; Ouidja, Mohand Ouidir; Sissoeff, Ludmilla; Raisman-Vozari, Rita; Papy-Garcia, Dulce; Univ Paris Est; Universidade Federal de São Paulo (UNIFESP); Univ Paris 06Glycosaminoglycans (GAGs) are major extracellular matrix components known to tightly regulate cell behavior by interacting with tissue effectors as trophic factors and other heparin binding proteins. Alterations of GAGs structures might thus modify the nature and extent of these interactions and alter tissue integrity. Here, we studied levels and composition of GAGs isolated from adult and aged human hippocampus and investigated if their changes can influence the function of important trophic factors and the A beta 42 peptide toxicity. Biochemical analyses showed that heparan sulfates are increased in the aged hippocampus. Moreover, GAGs from aged hippocampus showed altered capacities to regulate trophic factor activities without changing their capacities to protect cells from A beta 42 toxicity, compared to adult hippocampus GAGs. Structural alterations in GAGs from elderly were suggested by differential transcripts levels of key biosynthetic enzymes. C5-epimerase and 2-OST expressions were decreased while NDST-2 and 3-OST-4 were increased; in contrast, heparanase expression was unchanged. Results suggest that alteration of GAGs in hippocampus of aged subjects could participate to tissue impairment during aging. (C) 2012 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosTGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding(Wiley-Blackwell, 2014-10-01) Alves, Claudia Cristina; Torrinhas, Raquel Susana; Giorgi, Ricardo; Brentani, Maria Mitzi; Logullo, Angela Flavia [UNIFESP]; Waitzberg, Dan Linetzky; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. in experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets.