Navegando por Palavras-chave "HepG2"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Avaliação do impacto da co-exposição do Triclosan e do Ftalato dehp sobre a viabilidade celular e a estabilidade genética, in vitro
    (Universidade Federal de São Paulo (UNIFESP), 2020-01-15) Duarte, Nathalia De Assis Aguilar [UNIFESP]; Barcelos, Gustavo Rafael Mazzaron [UNIFESP]; Universidade Federal de São Paulo
    Triclosan (TCS) is an antimicrobial agent widely used in Personal Care Products (PCPs) and Di-(2-ethylhydroxyl-phtalate) (DEHP) is a phthalate, a group of chemical compounds derived from phthalic acid, used in medical devices and plastic products with Polyvinyl Chloride (PVCs). As a result of extensive use, TCS and DEHP has been found in the environment and previous studies have demonstrated the interaction between their exposure and genotoxic damage to aquatic organisms. However, there is a shortage in the literature of these effects on human cells. Therefore, the aim of this study is to evaluate the cytotoxicity, genotoxicity, mutagenicity and interaction between TCS and DEPH compounds in HepG2 human hepatocarcinoma cell culture. Three different concentrations of each drug were chosen using the MTT test (0,10 μM, 1 μM e 10 μM) and 8 different combinations were evaluated later (e.g. TCS 10 μM + DEHP 1μM). The number of micronuclei (MN), nucleoplasmic bridges (NPBs), nuclear buds (NBUDs), nuclear division index (NDI), nuclear division cytotoxic index (NDCI), apoptotic and necrotic cells were evaluated. The results have shown that the three concentrations of TCS and the two highest DEHP concentrations increased the number of MN (p<0,05) as well as their combinations. The lower concentrations of both drugs had lower NDI and NDCI (p<0,05) when compared to the control group (DMSO 1%). The compounds showed synergistic and antagonist effects in several parameters analyzed. Finally, the results have shown a significant mutagenic (increase of MN) and cytostatic (decrease of NDI) and cytotoxic (decrease of NDCI) damage especially with co-exposure treatments, which is a more realistic model than studying drugs separately.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Prospecção dos efeitos biológicos do Poligodial isolado da Drimys brasiliensis (Winteraceae) na via de sinalização da insulina e no estresse oxidativo em células hepáticas
    (Universidade Federal de São Paulo, 2015-08-31) Gomes, Moisés Felipe Pereira [UNIFESP]; Caperuto, Luciana Chagas [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    According Brazilian Diabetes Society (SBD, 2006), "Diabetes mellitus" is not a single disease but a heterogeneous group of metabolic disorders has in common hyperglycemia, which is the result of defects in insulin action in secretion or both. Sustained hyperglycemia promotes extensive deleterious effects and sometimes irreversible to the body as renal failure, retinopathy, cardiovascular and liver diseases, thereby impairing the regulation of metabolism of carbohydrates. Due to this insulin resistance in the early stages of type 2 diabetes mellitus (DM2), the sensitive lipase hormone (LHS) does not have its activity suppressed by this hormone, thus promoting increase in free fatty acids (FFA) Current that will be drained into the hepatic portal system, thus promoting non-alcoholic fatty liver disease (NAFLD). In traditional medicine, Drimys brasiliensis infusions are used for the treatment of diabetes. However, there are no reports on the polygodial (PGD), the major compound of D. brasiliensis, could be responsible for the hypoglycemic effect. Therefore, we analyzed cell viability by hepatocytes (HepG2) grown with PGD (30, 300 and 3000nM) and evaluate the antioxidant activity in cultured cells with 2-deoxy-D-ribose (2-drib) (50mM) for aindução the oxidative stress. We also study the effect on the insulin receptor, Akt and JNK, as well as their phosphorylated forms. Our results demonstrate that: 1) the PGD does not have antioxidant activity; 2) Treatment with PGD presents a trend in decreased expression of AKT and 3) has a synergistic effect with PGD 2-drib, leading to an increase in the phosphorylation of JNK. We conclude that diabetics who use tea D. brasiliensis may have worsening of symptoms due to the deleterious effect of PGD in enhanced oxidative stress.