Navegando por Palavras-chave "Hipoxia"

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    Modelo experimental de indução de lesão oxidativa hepática em ratos por halotano
    (Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED, 2007-03-01) Brasil, Luis Josino; Amaral, José Luiz Gomes do [UNIFESP]; Zettler, Claudio Galeano; Marroni, Claudio Augusto; Vercelino, Rafael; Marroni, Norma; Irmandade da Santa Casa de Misericórdia de Porto Alegre Serviço de Anestesiologia; Universidade Federal de São Paulo (UNIFESP); Fundação Faculdade de Ciências Médicas de Porto Alegre Departamento de Patologia; Fundação Faculdade de Ciências Médicas de Porto Alegre Departamento de Hepatologia; Universidade Federal do Rio Grande do Sul Hospital das Clínicas de Porto Alegre Laboratório de Fisiologia Experimental
    BACKGROUND: The anesthetic halothane can be reductively metabolized to reactives intermediates that may initiate lipid peroxidation accompanied by hepatic injury. Hypoxia and phenobarbital pretreatment in rats increases metabolism of halothane, the oxidative stress, cause liver antioxidant enzymes changes and tissue damage. AIMS: We investigated the effect of halothane on hepatic lipid peroxidation and on hepatic histology after increases reductive metabolism of halothane caused by hypoxia and phenobarbital pretreatment. METHODS: Twenty-five male wistar rats were divided in five equals groups: CO (Control), HO14 (Halothane/Hypoxia), F (fenobarbital alone), O14 (Hypoxia alone) and H (Halothane alone). After 24 hours the rats were killed, their livers removed to determine chemoluminescence, thiobarbituric acid-reactive substances, catalase, superoxide dismutase, and blood samples were taken to determine AST and ALT. The histopathologic evaluation was performed with hematoxylin and eosin staining. Histopathologic scores are presented as 25th-75th percentile/range values and median ± range. RESULTS/CONCLUSION: Halothane-hypoxic exposure resulted in a significant changes in the activities of antioxidant enzymes, and induced hepatic lipoperoxidation. Moreover it resulted in histopathologic liver injury as well as significant increase of serum activity of AST and ALT.