Navegando por Palavras-chave "IgG"

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    Antibody response against plasmid-encoded toxin (Pet) and the protein involved in intestinal colonization (Pic) in children with diarrhea produced by enteroaggregative Escherichia coli
    (Elsevier B.V., 2005-02-01) Bellini, E. M.; Elias Junior, Waldir Pereira [UNIFESP]; Gomes, Tania Aparecida Tardelli [UNIFESP]; Tanaka, T. L.; Taddei, C. R.; Huerta, R.; Navarro-Garcia, F.; Martinez, M. B.; Universidade de São Paulo (USP); Inst Butantan; Universidade Federal de São Paulo (UNIFESP); CINVESTAV
    Enteroaggregative Escherichia eoli (EAEC) is an emerging cause of pediatric and adult travellers diarrhea. the mechanism by which EAEC induce diarrhea is not completely known. Two serine protease autotransporter proteins, named Pet and Pic have been identified in EAEC strains. Pet has enterotoxic and cytotoxic activities, while the role of Pic in pathogenesis may lie on its mucinolytic activity. Little is known about Pet and Pic biological activities in vivo. in this study the antibody responses against these autotransporter proteins in convalescent children is investigated. Fifteen (83%) children showed specific antibodies against Pet or Pic in their sera. IgG and IgM antibodies were the main isotype found. Specific antibodies against Pic, but not against Pet, were detected in sera from age-matched control group. These data show that specific anti-Pet and anti-Pic antibodies are produced during the course of a natural EAEC infection in children. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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    Antigenuria and antigenaemia in experimental murine paracoccidioidomycosis
    (Taylor & Francis Ltd, 2005-11-01) Ramos, S. P.; Sano, A.; Ono, M. A.; Camargo, Z. P.; Estevao, D.; Miyaji, M.; Nishimura, K.; Itano, E. N.; Universidade Estadual de Londrina (UEL); Chiba Univ; Universidade Federal de São Paulo (UNIFESP)
    In this study, Swiss mice were experimentally infected with Paracoccidoides brasiliensis (Pb18) and we investigated the levels of gp43 in urine and plasma, anti-gp43 and IgG-gp43 immune complexes in plasma. These levels were correlated with the histopathological findings. Blood and urine samples were collected from mice at 7, 28, 56 and 84 days after intravenous inoculation of 105 yeast cells, and analysed by ELISA. the results showed increased levels of soluble gp43 in the plasma in all periods, and anti-gp43 IgG and immune complexes after day 28. High gp43 levels were detected in the urine, except for day 28, coincident with the presence of compact granulomas in lungs. All the infected mice showed fungal cells in the lungs, with initial granulomatous lesions at day 7, dissemination of lesions to other organs at day 56, and granulomas lacking the surrounding mononuclear cells infiltration, especially at days 56 and 84. Our results suggest that gp43 diffuses passively into the urine, and the determination of gp43 levels in urine samples may be a non-invasive alternative method for diagnosis and follow up of PCM. Further studies are needed to determine if the cellular immune response correlate with decreased urine gp43 levels.
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    Reactivity of antibodies from patients with acute and chronic paracoccidioidomycosis to a high molecular mass antigen from Paracoccidioides brasiliensis
    (Wiley-Blackwell, 2005-01-01) Marquez, A. S.; Vicentini, A. P.; Ono, M. A.; Watanabe, MAE; Camargo, Z. P. de; Itano, E. N.; Universidade Estadual de Londrina (UEL); Adolfo Lutz Inst; Universidade Federal de São Paulo (UNIFESP)
    Yeast forms of Paracoccidioides brasiliensis produce polydispersed high molecular mass (h-MM) antigens. We investigated the antibodies to an h-MM antigen from P. brasiliensis by immunoblotting and ELISA in sera from paracoccidioidomycosis (PCM) patients. IgG from the sera of chronic PCIVI patients was able to recognize the h-MM antigen at a higher frequency in the cell-free antigen (CFA) (8/13) than in the somatic antigen (SA) (2/13), as assessed by immunoblotting. the CFA was fractionated by Sephadex G-200 chromatography, and fraction 17 (F17) with the h-MM antigen of approximately 366 kDa was used in ELISA to analyze specific levels of IgG and IgE. Patients with the chronic form showed significantly higher levels of IgG (P < 0.05) but not IgE (P > 0.05) to F17 by ELISA, compared to patients with the acute form or to healthy donors. in conclusion, CFA is better than SA as a source of the P. brasiliensis h-MM antigen. This study reveals a new characteristic to differentiate between the acute and chronic forms of PCM, by demonstrating a higher level of seric IgG to h-MM antigen in chronic compared to acute PCM patients.
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    Serum anti-phenolic glycolipid1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?
    (Wiley, 2018) Macedo, Alexandre C. de; Guimaraes, Juliana A.; Rodrigues, Raphael O.; Araujo, Thiago D. V.; Tavares, Clodis M.; Cabral, Paula B.; Moraes-Pinto, Maria Isabel de [UNIFESP]; Nagao-Dias, Aparecida T.
    ObjectiveThe aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. MethodAnalysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. ResultsA strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MB patients. A moderate correlation was found in all analyses in PB patients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%
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    Transfer of IgG subclasses across placenta in term and preterm newborns
    (Assoc Bras Divulg Cientifica, 1996-02-01) Costa Carvalho, B. T.; Vieira, H. M.; Dimantas, RBR; Arslanian, C.; Naspitz, Charles Kirov [UNIFESP]; Solé, Dirceu [UNIFESP]; CarneiroSampaio, MMS; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    In order to study placental transfer of IgG subclasses, paired blood samples were collected from mothers and umbilical cord of preterm (N = 69) and full-term (N = 68) newborns. The full-term group was further divided into 3 subgroups: appropriate for gestational age (AGA, N = 43), large for gestational age (LGA, N = 13) and small for gestational age (SGA, N = 12), according to birth weight. IgC subclasses (IgG1, IgG2, IgG3 and IgG4) were measured by the single radial immunodiffusion technique using monoclonal antibodies. IgG1 and IgG3 newborn subclass concentrations (10.17 and 0.57 g/l, respectively) increased with increasing gestational age and reached maternal levels (IgG1 = 8.86; IgG3 = 0.67 g/l) during the 37th week of pregnancy. Low levels of these subclasses were found in premature newborns. IgG2 from newborns were always lower than maternal levels (P<0.05). LGA and SGA newborns had equivalent levels of IgG1 and IgG2 compared with AGA. SGA newborns had higher levels of lgG3 and lower levels of IgG4 than LGA and AGA newborns.
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    The Value of Specific IgE to Peanut and Its Component Ara h 2 in the Diagnosis of Peanut Allergy
    (Elsevier B.V., 2013-07-01) Lopes de Oliveira, Lucila C. [UNIFESP]; Aderhold, Martina; Brill, Marianne; Schulz, Gabriele; Rolinck-Werninghaus, Claudia; Mills, E. N. Clare; Niggemann, Bodo; Naspitz, Charles Kirov [UNIFESP]; Wahn, Ulrich; Beyer, Kirsten; Charite; Universidade Federal de São Paulo (UNIFESP); VBC Genom; Inst Food Res; German Red Cross Hosp Westend
    BACKGROUND: To avoid unnecessary oral food challenges, which are time consuming, stressful, and risky, improved in vitro diagnostic methods for food allergy such as component resolved diagnostics are still under investigation.OBJECTIVE: To investigate the role of whole peanut-and peanut-component (Ara h 1, Ara h 2, Ara h 3, Ara h 6 and Ara h 8)-specific IgE levels in the diagnostic procedure of peanut allergy as well as the diagnostic properties of peanut-specific IgG and IgG(4).METHODS: Sixty-one children underwent oral peanut challenge tests for diagnostic purposes irrespective of their peanut-specific IgE levels. Peanut-specific serum IgE, IgG, and IgG(4) levels were determined by ImmunoCAP FEIA and specific IgE against individual peanut proteins by Immuno Solid-phase Allergen Chip.RESULTS: Thirty-four of 61 patients (56%) had a peanut allergy. No significant difference was observed for peanut-specific IgG or peanut-specific IgG(4) levels between patients who were allergic and tolerant patients, whereas peanut-specific IgE was significant higher in patients who were allergic than in tolerant patients (P < .005). Twenty-five of 61 children had peanut-specific IgE above a previously proposed cutoff level of 15 kU(A)/L; however, 7 of these 25 children (28%) were clinically tolerant. Ara h 2-specific IgE was significantly lower in tolerant than in patients with allergies (P < .0001). Interestingly, 94% of the patients with peanut allergies showed IgE-binding to Ara h 2. Unfortunately, 26% of the sensitized but tolerant patients have shown IgE-binding to Ara h 2 too.CONCLUSIONS: Neither the level of specific IgE to peanut nor to Ara h 2 was able to clearly distinguish patients with clinical relevant peanut allergy from those who were clinical tolerant in our population. As expected, peanut-specific IgG and IgG(4) did not improve the diagnostic procedure. (C) 2013 American Academy of Allergy, Asthma & Immunology