Navegando por Palavras-chave "Interleukin-1"
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- ItemSomente MetadadadosGuidelines for the management and treatment of periodic fever syndromes familial mediterranean fever(Elsevier science inc, 2016) Terreri, Maria Teresa R. A. [UNIFESP]; Bernardo, Wanderley Marques; Len, Claudio Arnaldo [UNIFESP]; Almeida da Silva, Clovis Artur; Ribeiro de Magalhaes, Cristina Medeiros; Sacchetti, Silvana B.; Leme Ferriani, Virginia Paes; Petry Piotto, Daniela Gerent [UNIFESP]; Cavalcanti, Andre de Souza; de Moraes, Ana Julia Pantoja; Sztajnbok, Flavio Roberto; Feitosa de Oliveira, Sheila Knupp; Arruda Campos, Lucia Maria; Bandeira, Marcia; Sena Teixeira Santos, Flavia Patricia; Magalhaes, Claudia SaadObjective: To establish guidelines based on scientific evidence for the management of familial Mediterranean fever. Description of the evidence collection method: The Guideline was prepared from 5 clinical questions that were structured through PICO (Patient, Intervention or indicator, Comparison and Outcome), to search in key primary scientific information databases. After defining the potential studies to support the recommendations, these were graduated considering their strength of evidence and grade of recommendation. Results: 10,341 articles were retrieved and evaluated by title and abstract
- ItemAcesso aberto (Open Access)Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-08-01) Gragnani, Alfredo [UNIFESP]; Müller, Bruno Rafael [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Noronha, Samuel Marcos Ribeiro de [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To evaluate the gene expression of KGF, TNF-alpha and IL-1 beta in skin fibroblasts and keratinocytes cultured from burned patients. METHODS: Three patients with large burns and three patients with small burns, as well as two controls, were included. The cell culture was initiated by the enzymatic method. After extraction and purification of mRNA, qPCR was used to assess the gene expression of KGF, TNF-alpha and IL-1 beta. RESULTS: The expression of KGF was increased on average 220-fold in large burns and 33.33-fold in small burns in fibroblasts, and 11.2-fold in large burns and 3.45-fold in small burns in keratinocytes compared to healthy patients (p<0.05). Expression of TNF-alpha was not observed. IL-1 beta is down-regulated in fibroblasts of burned patients, and much more repressed in small burns (687-fold, p<0.05). In keratinocytes, the repression of IL-1 beta expression occurs in patients with small burns (28-fold), while patients with large burns express this gene intensively (15-fold). CONCLUSIONS: The study showed a quantitative pattern in the expression of KGF gene, which is more expressed according to the size of the burn. TNF-alpha was not expressed. A qualitative pattern in the expression of IL-1 beta gene was demonstrated.
- ItemSomente MetadadadosLow-level laser therapy prevents degenerative morphological changes in an experimental model of anterior cruciate ligament transection in rats(Springer, 2014-09-01) Bublitz, Caroline [UNIFESP]; Medalha, Carla Christina [UNIFESP]; Oliveira, Poliani de [UNIFESP]; Assis, Livia [UNIFESP]; Milares, Luiz Paulo [UNIFESP]; Fernandes, Kelly Rossetti [UNIFESP]; Tim, Carla Roberta [UNIFESP]; Vasilceac, Fernando Augusto [UNIFESP]; Mattiello, Stela Márcia [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The aim of this study was to analyze the effects of low-level laser therapy (LLLT) on the prevention of cartilage damage after the anterior cruciate ligament transection (ACLT) in knees of rats. Thirty male rats (Wistar) were distributed into three groups (n = 10 each): injured control group (CG); injured laser-treated group at 10 J/cm(2) (L10), and injured laser-treated group at 50 J/cm(2) (L50). Laser treatment started immediately after the surgery and it was performed for 15 sessions. An 808 nm laser, at 10 and 50 J/cm(2), was used. To evaluate the effects of LLLT, the qualitative and semi-quantitative histological, morphometric, and immunohistochemistry analysis were performed. Initial signs of tissue degradation were observed in CG. Interestingly, laser-treated animals presented a better tissue organization, especially at the fluence of 10 J/cm(2). Furthermore, laser phototherapy was able of modulating some of the aspects related to the degenerative process, such as the prevention of proteoglycans loss and the increase in cartilage area. However, LLLT was not able of modulating chondrocytes proliferation and the immunoexpression of markers related to inflammatory process (IL-1 and MMP-13). This study showed that 808 nm laser, at both fluences, prevented features related to the articular degenerative process in the knees of rats after ACLT.
- ItemSomente MetadadadosPolimorfismos de genes dos receptores de progesterona, do receptor de IL-1 e do VEGF e o aborto espontâneo de repetição(Universidade Federal de São Paulo (UNIFESP), 2008) Traina, Evelyn [UNIFESP]; Mattar, Rosiane [UNIFESP]Objetivo: avaliar se polimorfismos de genes que codificam a expressao do receptor de progesterona (PROGINS), do receptor de IL-1 (Pst I) e do fator de crescimento endotelial vascular (-634C/G e 936C/T) podem estar relacionados a ocorrencia de aborto espontaneo de repeticao. Pacientes e metodos: o estudo tem carater caso-controle. Para o grupo caso foram selecionadas pacientes com antecedente de pelo menos tres abortos espontaneos consecutivos (AER), sem etiologia definida. O grupo controle foi composto por mulheres com pelo menos duas gestacoes de termo sem intercorrencias e sem antecedentes de abortamento. Realizada coleta de sangue por puncao venosa periferica e extracao do DNA pelo metodo DTAB/CTAB. As genotipagens foram realizadas por reacao de polimerase em cadeia, seguida de diGestão por enzima de restricao, quando necessario. Resultados: 89 pacientes foram incluidas no grupo caso e 191 no grupo controle. As frequencias encontradas para o polimorfismo PROGINS foram de 72,3% T1/T1 e 27,7% T1/T2 no grupo com AER e 76,4% T1/T1, 22,3% T1/T2 e 1,3% T2/T2 no grupo controle. Nao houve diferencas entre os grupos, analisando-se as frequencias genotipicas (p=0,481) e alelicas (p=0,656). Para o polimorfismo Pst I: grupo com AER − 19,5% CC, 58,4% CT e 22,1% TT; grupo controle − 23,2% CC, 57,4% CT, 19,4% TT. Nao foram encontradas diferencas significantes considerando-se os modelos de heranca co-dominante (p=0,769), dominante (p=0,517) e alelos (p=0,512). Os genotipos encontrados para o polimorfismo -634C/G foram: 22,1% CC, 48,1% CG e 29,9% GG no grupo caso e 12,9% CC, 55,3% CG e 31,8% GG no grupo controle. Os valores encontrados para analises em modelos co-dominante, dominante e frequencias alelicas foram respectivamente de p=0,300; p=0,125 e p=0,317. Na analise do polimorfismo 936C/T, as frequencias do grupo com AER foram de 73,8% CC, 25,0% CT, 1,3% TT e no grupo controle, 78,3% CC, 20,9% CT, 0,8% TT, resultando valores de p=0,451 e p=0,446, respectivamente, para analise dos genotipos em modelo dominante e de alelos. Conclusoes: Nao foi observada associacao entre os polimorfismos de genes que codificam a expressao de receptores de progesterona (PROGINS), de receptor de IL- 1 (Pst I) e de VEGF (-634C/G e 936C/T) e a ocorrencia de aborto espontaneo de repeticao
- ItemSomente MetadadadosPolymorphisms in VEGF, progesterone receptor and IL-1 receptor genes in women with recurrent spontaneous abortion(Elsevier B.V., 2011-01-01) Traina, Evelyn [UNIFESP]; Daher, Silvia [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Sun, Sue Yazaki [UNIFESP]; Franchim, Camila Sommerauer [UNIFESP]; Mattar, Rosiane [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Hormonal, inflammatory and vascular alterations during pregnancy are thought to be involved in pregnancy loss. the role of progesterone, the actions of which involve cytokines and vascular endothelial growth factor (VEGF), has been thoroughly studied. Genetic factors are involved in modulation of these molecules, and several genes have been associated with recurrent spontaneous abortion (RSA), as well other obstetric conditions. Here we investigate the relationship between RSA and gene polymorphisms of the progesterone receptor (PROGINS), interleukin-1 receptor 1 (PstI) and vascular endothelial growth factor (VEGF) (-634, 936). This case-controlled study comprised a total of 89 women with idiopathic RSA and a control group of 191 women with at least two successful pregnancies and no miscarriages. Genomic DNA was extracted from whole blood, and polymorphism genotyping was conducted by digesting PCR products with specific restriction endonucleases. No correlations were found in any of the investigated polymorphisms, even among dominant, co-dominant and additive inheritance models and alleles. Our results support the hypothesis that these specific gene polymorphisms are not the major determinant of pregnancy success. Although we did not find any correlations, the investigation of these and other polymorphisms remains a challenge in the evaluation of RSA. (C) 2010 Elsevier Ireland Ltd. All rights reserved.