Navegando por Palavras-chave "Ischemic Preconditioning"
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- ItemAcesso aberto (Open Access)Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-10-01) Neves, José de Souza [UNIFESP]; Abrahão, Marcos de Souza [UNIFESP]; Salzedas-Netto, Alcides Augusto [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Gonzalez, Adriano Miziara [UNIFESP]; University of Mato Grosso Department of Surgery; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To evaluate the effects of ischemic preconditioning (IPC) associate with different preservation solutions, in the protecting of gut. METHODS: Four groups of 14 rats underwent laparotomy and collecting 20 cm of ileum, for preservation, at 4ºC, in Belzer (Belz), Ringer (RL), Celsior (Cs) and Custodiol (Cust) solutions, for 24 hours. Prior to collection, half of the animals in each group were subjected to IPC. During preservation, in the periods of zero, 12, 18 and 24 hours, were conducted evaluating the degree of mucosal injury and dosage of malondialdehyde acid (MDA). RESULTS: In all periods the RL group, with and without IPC, presented MDA values higher than the Belz and Cs. The degree of mucosal injury in the non-ipc RLgroup with 12h preservation was higher than the others; with 18 and 24h, the RL and Cust had higher degrees of damage than Cs and Belz. With IPC, in all periods, the group Cs and Belz had lower degrees of injury. CONCLUSION: The Celsior and Belzer solutions had better protective effects on the gut and these effects were enhanced by IPC.
- ItemAcesso aberto (Open Access)The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline(Acta Cirurgica Brasileira, 2017) Ribeiro de Oliveira, Teresinha Regina; de Oliveira, Geraldo Ferreira; Simoes, Ricardo Santos; Feitosa, Suellen Maurim [UNIFESP]; Tikazawa, Eduardo Hiroshi; Monteiro, Hugo Pequeno [UNIFESP]; Fagundes, Djalma Jose [UNIFESP]; Taha, Murched Omar [UNIFESP]Purpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+ R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes). Results: The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P< 0.05) compared to IR+ SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P> 0.05). The NOS-2 expression (qRTPCR) in the IRPTX group (P< 0.05) was higher than the values for the IPC+ IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P< 0.05), the IR-SS (P< 0.05) and the IR-PTX (P< 0.05) groups. Conclusions: The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect.
- ItemSomente MetadadadosIschemic preconditioning attenuates remote pulmonary inflammatory infiltration of diabetic rats with an intestinal and hepatic ischemia-reperfusion injury(Acta Cirurgica Brasileira, 2013-03-01) Thomaz Neto, Farid Jose; Koike, Marcia Kiyomi [UNIFESP]; Abrahão, Marcos de Souza [UNIFESP]; Carillo Neto, Francisco; Pereira, Renan Kenji Hanada; Machado, Jose Lucio Martins; Montero, Edna Frasson de Souza [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); IAMSPEPURPOSE: To assess ischemic preconditioning (IPC) effects in pulmonary lesion in intestinal and hepatic ischemia-reperfusion (IR) injury models using diabetic rats.METHODS: Diabetes (DM) was induced in 28 male Wistar rats by alloxan (42 mg/kg, IV). After 28 days, severe DM rats were submitted to intestinal or hepatic IR injury with or without IPC. Intestinal IR (30 min of mesenteric artery occlusion and 30 min of reperfusion; n=6) and IPC groups (10 min ischemia, 10 min reperfusion, followed by intestinal IR; n=6), and Hepatic IR (30 min of hepatic pedicle occlusion and 30 min of reperfusion; n=5) and IPC groups (10 min ischemia, 10 min reperfusion, followed by hepatic IR; n=5), were compared to DM rats group (n=6). Plasmatic lactate, glycemia were measured before and after IR injury. Histomorphology of lung was performed counting inflammatory cells. Data was expressed in mean +/- SE. P<0.05.RESULTS: Glycemia and lactate were similar among groups. IPC did not interfere in these parameters. On histological evaluation, IR increased inflammatory cells infiltration in pulmonary parenchyma compared to control in both IR injury models. IPC attenuated inflammatory infiltration in lungs.CONCLUSION: Ischemic preconditioning protects against remote ischemia-reperfusion injury in lung on intestinal or hepatic ischemia-reperfusion model with acute diabetes.
- ItemAcesso aberto (Open Access)Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant(Acta Cirurgica Brasileira, 2017) Luciano Gomes, Rudnei de Oliveira [UNIFESP]; Neto, Ricardo Artigiani [UNIFESP]; Guimaraes Neto, Jose de Freitas [UNIFESP]; Nunes, Adriana Porto [UNIFESP]; de Souza Montero, Edna Frasson [UNIFESP]; Martins, Jose Luiz [UNIFESP]Purpose: To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. Methods: We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) - Newborn rats born from a pregnant rat which did not undergo any intervention
- ItemAcesso aberto (Open Access)Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice(Acta Cirurgica Brasileira, 2016) Morello, Ricardo José [UNIFESP]; Koike, Marcia Kiyomi; Abrahão, Marcos de Souza [UNIFESP]; Saad, Karen Ruggeri; Saad, Paulo Fernandes; Montero, Edna Frasson de Souza [UNIFESP]PURPOSE: To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model. METHODS: Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically. RESULTS: The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2+/-0.4 vs 9.0+/-0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2+/-0.7 and 10.2+/-0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0+/-0.8, 9.0+/-1.2, 0.0+/-0.0, 0.5+/-0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2+/-0.8 vs Tx: 8.8+/-0.9, IPC-R: 8.0+/-0.4 and Fk: 9.0+/-0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx. CONCLUSION: Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.
- ItemAcesso aberto (Open Access)The role of ischemic preconditioning and pentoxifylline in intestinal ischemia/reperfusion injury of rats(Acta Cirurgica Brasileira, 2017) Ribeiro de Oliveira, Teresinha Regina [UNIFESP]; de Oliveira, Geraldo Ferreira; Simoes, Ricardo Santos [UNIFESP]; Tikazawa, Eduardo Hiroshi; Monteiro, Hugo Pequeno [UNIFESP]; Fagundes, Djalma Jose [UNIFESP]; Taha, Murched Omar [UNIFESP]Purpose: To investigate the role of ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal mucosa ischemia/reperfusion injury (IR). Methods: Thirty rats were assigned to 5 groups (N= 6): (CG): no clamping of the superior mesenteric artery (90 min.)
- ItemAcesso aberto (Open Access)Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-10-01) Damous, Luciana Lamarão [UNIFESP]; Silva, Sônia Maria da [UNIFESP]; Lopes, Renato Antônio Migliano [UNIFESP]; Sakano, Célia Regina de Souza Bezerra [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. METHODS: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. RESULTS: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). CONCLUSION: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.