Navegando por Palavras-chave "Lesões da medula espinal"

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    Terapia a laser de baixa intensidade na neuroplasticidade e recuperação funcional da lesão medular
    (Universidade Federal de São Paulo (UNIFESP), 2016) Campo, Paula Fernanda Gallani Martin Del [UNIFESP]; Aguiar, Odair [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The spinal cord injury (SCI) consists of any damages to the structures in the medullary canal. This injury can be traumatic or non-traumatic, the latter being the most common. The central nervous system (CNS) has a low ability to get repaired, requiring the use of therapeutic approaches in order to favor neuroplasticity. In this context, low-level laser therapy (LLLT) has been proposed as a promising treatment for repairing central and peripheral nervous system injuries. Aim: to investigate the effects of LLLT, after traumatic injuries, on the regeneration and neuroplasticity of the spinal cord, as well as on the sensorial and motor functional recovery. Materials and methods: female wistar rats (n= 33), aging 10 weeks, were submitted to spinal cord injury contusion model (CEUA N: 9329290514). The animals were divided into two groups: control (C; n= 19) and laser (L; n= 14), and they were euthanized after 4 and 8 weeks. The L group was treated, for 15 consecutive days, with continuous emission of GaAlAs laser which was adjusted with the following parameters: λ = 808 nm, power of 100 mW, spot area = 0.028 cm²; t = 4'42 ''; dosage of 1000 J / cm²; E = 28 J; irradiance = 3.57 mW / cm². At days 1, 14, 28 and 56 after the surgery, the motor function (using BBB score and computed kinematics), the thermal sensitivity (using ice) and the tactile sensitivity (using Von Frey® monofilaments) were evaluated. Additionally, histological and immunohistochemical analysis were performed to investigate the lesion volume and the expression of GFAP respectively. Results: The main findings of this study demonstrate, based on the BBB score, that LLLT was able to improve the motor performance in the group L compared to group C after 56 days. Furthermore, LLLT was capable to decrease the immunoexpression of of GFAP marker and the lesion volume in the injury site after 56 days. Conclusion: Based on the results, it is suggested that the effect of LLLT in the lesion (i) assisted in the motor recovery; (ii) reduced the lesion volume and (iii) decreased the GFAP immunostaining. This study indicates that the LLLT is a promising therapeutic approach for the SCI treatment, justifying the need of further investigations utilizing other experimental models and other specific markers.