Navegando por Palavras-chave "MPTs Em Histonas"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Identificação do perfil global de modificações pós-traducionais em histonas e de proteínas associadas à cromatina na progressão do melanoma
    (Universidade Federal de São Paulo (UNIFESP), 2021) Pessoa, Guilherme Cavalcante [UNIFESP]; Jasiulionis, Miriam Galvonas [UNIFESP]; Universidade Federal de São Paulo
    Cutaneous melanoma, resulting from the malignant transformation of melanocytes, is the most aggressive type of skin tumors since it is highly metastatic. Its incidence has been increasing worldwide. It has been shown that changes in epigenetic marks, including posttranslational changes (MPTs) in histones, are involved in tumor development and progression, but studies of these changes in different stages of melanoma progression are still scarce. In nucleosomes, DNA is associated with histones, and MPTs in histones mediated by enzymes and multiprotein complexes interfere in this association. These interactions can trigger secondary mechanisms, such as the recruitment and activation of other proteins, remodeling chromatin and altering gene expression. Thus, changes in proteins associated with chromatin and in MPTs in histones impact the chromatin remodeling, and some of these MPTs can occur at sites in the genome associated with tumor development. A linear model consisting of cell lines that correspond to different stages of melanoma progression has been established in our laboratory (melanocytes melan-a, pre-malignant melanocytes 4C, non-metastatic melanoma cells 4C11- and metastatic 4C11 +). The objective of this work was to identify and quantify MPTs in histones and proteins associated with chromatin in four cell lines of our melanoma model. The identification of histone MPTs and chromatin-associated proteins was performed by mass spectrometry analysis. The results revealed histone modifications and chromatinassociated proteins potentially correlated with malignant transformation, EMT and metastasis in melanoma. These studies provided valuable data that may indicate potential new targets for melanoma treatment, in addition to promising prognostic markers.