Navegando por Palavras-chave "Membrane-Glycoproteins"

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    Quantification, 2de analysis and identification of enriched glycosylated proteins from mouse muscles: difficulties and alternatives
    (Wiley-blackwell, 2016) Menegoci Eugenio, Patricia de Fatima; Assunção, Nilson Antonio [UNIFESP]; Sciandra, Francesca; Aquino, Adriano; Brancaccio, Andrea; Carrilho, Emanuel
    One of the problems with 2DE is that proteins present in low amounts in a sample are usually not detected, since their signals are masked by the predominant proteins. The elimination of these abundant proteins is not a guaranteed solution to achieve the desired results. The main objective of this study was the comparison of common and simple methodologies employed for 2DE analysis followed by MS identification, focusing on a pre-purified sample using a wheat germ agglutinin (WGA) column. Adult male C57Black/Crj6 (C57BL/6) mice were chosen as the model animal in this study