Navegando por Palavras-chave "Moa-Mb-23"

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    Ação Do Inibidor De Tripsina Isolado De Sementes De Enterolobium Contortisiliquum Sobre A Linhagem De Células De Câncer De Mama Mda-Mb-231
    (Universidade Federal de São Paulo (UNIFESP), 2017-08-31) Lobo, Yara Aparecida [UNIFESP]; Oliva, Maria Luiza Vilela [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Breast cancer is the most frequent type among women and one of the leading causes of death in the world. MOA-MB-231 is a hormone-independent human breast cancer cells and has least favorable prognosis. In tumor progression, the activity of proteinases is involved in the cellular processes of proliferation, invasion and metastasis, so inhibitors of these enzymes have emerged as potential therapeutic targets. EcTI is a Kunitz-type serine proteinases inhibitor isolated from Enterolobium contortisiliquum seeds with effective antitumor action on several cell lines. The objective of this study was to evaluate the action of EcTI on the breast cancer MOA-MB-231 cellline. The EcTI was tested on cell viability, proliferation, adhesion, migration and invasion and the understanding of its effect investigated by the expression of integrins, cytokines and proteolytic enzymes, as well as cell signaling. The inhibitor blocked adhesion of these cells to collagen I (reduction - 30%) as well as migration (60%) and invasion (70%), inhibited the expression of a6 and [34 integrin subunits decreasing the phosphorylation of FAK and ERK and Akt, pathways related to cell proliferation, survival, adhesion, migration, and invasion. In addition, IL-6 and consequently, NFKB, overexpressed in cancer, IL-8 'cytokine, involved in survival and metastasis processes, and proliferation-related cytokine TGF-a, were decreased. Regarding to proteinase expression, the EcTI decreases the TPSA and MCP-1, which participate in the degradation of the ECM. Finally, the EcTI increased the content of heparan and chondroitin sulfate. Although the mechanism involved in this process has not yet been elucidated, the effect suggests that the increase of this proteoglycan may have amplified the adhesive process of MOA - MB - 231 cells, decreasinq the metastatic potential. In this way, EcTI is able to interfere in the complex triple negative cell signaling system, being an important instrument for understanding the structural requirements involved in establishing the aggressiveness of this type of tumor, indicating that this protein may contribute to the understanding of the tumor etiology.