Navegando por Palavras-chave "Molecular diagnostic techniques"

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    Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis
    (Conselho Brasileiro de Oftalmologia, 2009-10-01) Bispo, Paulo José Martins [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Pignatari, Antonio Carlos Campos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Bacterial endophthalmitis is a serious but uncommon intraocular infection which frequently results in vision loss. Early diagnosis and appropriate therapy are associated with better visual outcome. Conventional microbiological methods are currently used for microbiological characterization of eyes with suspected endophthalmitis. However, the sensitivity of bacterial detection from aqueous and vitreous humor using microbiology techniques is poor, and time-consuming to confirm the results. The application of molecular methods enhances significantly laboratory confirmation of bacterial endophthalmitis, demanding a shorter time to draw a definitive result and thereby promoting the early initiation of a more specific therapy to limit the empirical or unnecessary use of broad-spectrum antibiotics. PCR-based techniques, including post-PCR methods such RFLP, DNA probe hybridization and DNA sequencing have been successfully used for the diagnostic elucidation of clinically suspected bacterial endophthalmitis cases, showing promising application in the routine practice of ocular microbiology laboratories.
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    The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients
    (Soc Brasileira Dermatologia, 2013-01-01) Dantas, Katia Cristina; Freitas, Roseli S.; Moreira, Adriana Pardini Vicentini [UNIFESP]; Silva, Marcos Vinicius da; Benard, Gil; Vasconcellos, Cidia; Criado, Paulo Ricardo; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); State Hlth Secretariat; Emilio Ribas Infectol Inst; Insitute State Publ Employees IAMSPE
    The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.