Navegando por Palavras-chave "Monoterpenes"

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    Avaliação dos efeitos dos componentes dos óleos essenciais (timol, carvacrol e p-cimeno) no enfisema pulmonar induzido por instilação de elastase em camundongos
    (Universidade Federal de São Paulo, 2016-03-23) Pereira, Ellen Games Jacob [UNIFESP]; Prado, Carla Maximo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Pulmonary emphysema is the most important component of Chronic Obstructive Pulmonary Disease (COPD) and is characterized by destruction of alveolar walls resulting in an enlargement and progressively airflow limitation. Although COPD represents the fourth leading cause of mortality in the world there is still no specific therapeutic measures for the treatment, which is still symptomatic. Phytotherapy, including essential oils, is widely used to treat various diseases, however, in most cases, there is not enough scientific evidence. Objective: To evaluate the effects of treatment with thymol, carvacrol and p-cymene, monoterpenes derived from essential oils of Brazilian plant species, the changes in respiratory mechanics and pulmonary histopathology induced by instillation of elastase. Methodology: Male C57BL6 mice were used, which were given saline or elastase. 30 minutes after instillation of elastase animals were treated with thymol, carvacrol, p-cymene or vehicle. These treatments were repeated on days 7, 14, 21 and 28. Animals that received saline were treated with vehicle only. On the 28th, after 30 minutes of treatment they were evaluated: mechanics of the respiratory system; exhaled nitric oxide (NoEx), pulmonary inflammation and cytokines in the bronchoalveolar lavage (BAL), evaluation of the content of collagen fibers and alveolar diameter and expression of MMP-9, TIMP-1, NF-kB and 8-iso-PGF- 2? by immunohistochemistry. Statistical analysis was performed using the SigmaStat using analysis of variance One way ANOVA. Results: The elastase group had pulmonary emphysema (increase of Lm), an increase of macrophages and IL-6, IL-8, IL-1? and IL-17 levels in BAL, in addition to increased deposition of fibers collagen and expression of MMP-9, TIMP-1, isoprostane and NF-kB in the lung parenchyma. Still, there was an increase of NoEx these animals. The treatments with thymol, carvacrol and p-cymene reduced pulmonary inflammation, the cytokines IL-6, IL-8, IL-17 and IL-1? the values Lm and collagen deposition in the lung parenchyma, as well as MMP-9 expression, NF-KB and isoprostane in lung tissue. TIMP 1 levels in the treated groups were higher than the control group but similar to animals receiving no treatment elastase. Treatment with thymol still reduced exhaled NO. Conclusion: These compounds reduced the inflammation and tissue destruction attenuated and reduced collagen deposition associated with MMP-9 reduced. These effects can be attributed to reduced NF-kB expression of pro-inflammatory cytokines (IL-17, IL-1?) and also reduce oxidative stress. Apparently, the position or the hydroxyl radical presence does not interfere with the biological effects of these compounds in the emphysema model. Other mechanisms of action have yet to be evaluated.
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    Estudo da interação do gama-terpineol em filmes de Langmuir como modelos de membrana
    (Universidade Federal de São Paulo, 2021-01-29) Jaroque, Guilherme Nuñez [UNIFESP]; Caseli, Luciano [UNIFESP]; Sartorelli, Patricia [UNIFESP]; http://lattes.cnpq.br/6836392358779448; http://lattes.cnpq.br/8929162910172931; http://lattes.cnpq.br/3306052344968821
    This work aimed to study the interaction of γ-terpineol with lipid Langmuir monolayers, which served as a model for cell membranes to analyze the possible biological actions of the compound at the molecular level. The phospholipids used to compose the films were dipalmitoylphosphatidylcholine (DPPC), dipalmitoyl phosphatidylethanolamine (DPPE), pure or mixed with cholesterol or with bacterial peptidoglycan. The lipids were spread on the air-water interface through organic solutions assembling a Langmuir monolayer. The γ-terpineol compound was incorporated in the monolayer by co-spreading it with the lipids. The interfacial films formed at the air-water interface were compressed by movable barriers, reaching surface pressures that approached the viscoelastic and thermodynamic properties of a proteolipid bilayer. The interaction of the drug with the monolayers was analyzed using tensiometric measurements (surface pressure- area isotherms, tensiometric stability, hysteresis assays), Brewster angle microscopy, and polarization-modulation infrared reflection-adsorption spectroscopy. The physicochemical properties obtained by these measurements were analyzed, and the interactions of γ-terpineol with the lipids at the air-water interface were inferred and extrapolated for the action of the compound in related cell membranes.
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    Structurally Related Monoterpenes p-Cymene, Carvacrol and Thymol Isolated from Essential Oil from Leaves of Lippia sidoides Cham. (Verbenaceae) Protect Mice against Elastase-Induced Emphysema
    (Mdpi Ag, 2016) Games, Ellen [UNIFESP]; Guerreiro, Marina [UNIFESP]; Santana, Fernanda Paula Roncon [UNIFESP]; Pinheiro, Nathalia Montouro; Oliveira, Emerson Alves de [UNIFESP]; Lopes, Fernanda Degobbi Tenorio Quirino dos Santos; Olivo, Clarice Rosa; Tibério, Iolanda de Fátima Lopes Calvo; Martins, Milton de Arruda; Lago, Joao Henrique Ghilardi [UNIFESP]; Prado, Carla Máximo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and inflammation. Natural products, such as monoterpenes, displayed anti-inflammatory and anti-oxidant activities and can be used as a source of new compounds to COPD treatment. Our aim was to evaluate, in an elastase-induced pulmonary emphysema in mice, the effects of and underlying mechanisms of three related natural monoterpenes (p-cymene, carvacrol and thymol) isolated from essential oil from leaves Lippia sidoides Cham. (Verbenaceae). Methods: Mices received porcine pancreatic elastase (PPE) and were treated with p-cymene, carvacrol, thymol or vehicle 30 min later and again on 7th, 14th and 28th days. Lung inflammatory profile and histological sections were evaluated. Results: In the elastase-instilled animals, the tested monoterpenes reduced alveolar enlargement, macrophages and the levels of IL-1 beta, IL-6, IL-8 and IL-17 in bronchoalveolar lavage fluid (BALF), and collagen fibers, MMP-9 and p-65-NF-kappa B-positive cells in lung parenchyma (p < 0.05). All treatments attenuated levels of 8-iso-PGF2 alpha but only thymol was able to reduced exhaled nitric oxide (p < 0.05). Conclusion: Monoterpenes p-cymene, carvacrol and thymol reduced lung emphysema and inflammation in mice. No significant differences among the three monoterpenes treatments were found, suggesting that the presence of hydroxyl group in the molecular structure of thymol and carvacrol do not play a central role in the anti-inflammatory effects.