Navegando por Palavras-chave "Muscle, Skeletal"
Agora exibindo 1 - 5 de 5
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)Blockage of intercellular adhesion molecule-1 (ICAM-1) in the prevention of reperfusion lesion in the skeletal musculature of EPM-1 Wistar rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2004-12-01) David Filho, Roberto [UNIFESP]; Moraes, Marcelo Rodrigo de Souza [UNIFESP]; Baptista-Silva, José Carlos Costa [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Ulian, Marcela [UNIFESP]; Miranda Júnior, Fausto [UNIFESP]; Burihan, Emil [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: Ischemia-reperfusion lesions are a form of acute inflammation in which leukocytes are considered to play a pivotal role. This study was made with the objective of determining whether the blockage of intracellular adhesion molecule-1, involved in the diapedesis of leukocytes, is efficacious in minimizing this lesions in the skeletal musculature of the posterior limbs of rats. Methods: The juxta-infrarenal aorta of three groups of six adult rats was clipped for six hours. After this, one group was sacrificed (control group) and the others underwent 24 hours of reperfusion, one with 0.9% physiological saline (reperfusion group) and the other with anti-ICAM-1 monoclonal antibodies (ICAM-1 group). A myeloperoxidase assay was utilized for estimating the infiltrate of neutrophils. Biopsies were obtained to make thin sections of hematoxylin-eosin and NADH. Blood samples were collected for making assays of biochemical parameters (creatinine; potassium; DHL; leukogram; venous pH; CK). Results: The myeloperoxidase levels were raised in the reperfusion (p < 0.001) and ICAM-1 (p < 0.019) groups in relation to the control group. The oxidative activity of the muscle fibers was significantly raised in the groups that underwent reperfusion. The other parameters did not present significant differences. Conclusions: The reperfusion lesion was bigger than the ischemic lesion. There was an increase in oxidative activity and inflammatory infiltrate with the reperfusion, without significant muscle necrosis being seen under the optical microscope. The blockage of ICAM-1 diminished the inflammatory infiltrate but not the rise in oxidative activity observed with the reperfusion.
- ItemAcesso aberto (Open Access)The effects of pentoxifylline into the kidneys of rats in a model of unilateral hindlimb ischemia/reperfusion injury(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2008-02-01) Teruya, Roberto; Fagundes, Djalma José [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Brasileiro, José Lacerda; Marks, Guido; Ynouye, Celso Massachi; Simões, Manuel de Jesus [UNIFESP]; Federal University of Mato Grosso do Sul Surgery Clinic Department; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To study the role of pentoxifylline (PTX) on remote kidney injury caused by muscle ischemia of left hindlimb of rats. METHODS: After xylazine and ketamine anesthesia, the left hindlimb of rats (n=66) were submitted to 6 hours ischemia (clamping the left common iliac artery). Three groups were used: sham group (SG, n=6), early group (EG, n=30) with reperfusion after 4 hours and late group (LG, n=30) with reperfusion after 24 hours. The saline solution (EG1, n=10 and LG1, n=10) or PTX (40mg.Kg-1) was administered in the reperfusion beginning (EG2, n=10/LG2, n=10) or divided in two doses in the ischemia beginning and reperfusion beginning (EG3, n=10/LG3, n=10). The plasmatic creatinokinase, urea, creatinine, sodium and potassium values were measure and histological samples from left kidney were prepared and H&E stained for scored cellular necrosis and degeneration of kidney tubules and thickness glomerulus determination. The apoptosis index was determined by immunohistochemical expression of the caspase-3. The tests of Mann-Whitney and Kruskal-Wallis (p < 0.05) were applied. RESULTS: The urea (90.5 ± 30.96 mg.dL-1), creatinine (2.28 ± 0.54 mg.dL-1), potassium (16 ± 3.66 mmol.dL-1) and mesangium thickness (0.97 ± 0.42 µm) values were significantly higher in group LG3. There was no significantly difference of caspase 3 expression between EG2 (16.35 ± 1.65%) and LG3 (15.57 ± 2.54%), and both were significantly worse than SG (9.8 ± 1.98%). CONCLUSIONS: The PTX has some protecting effect on remote kidney injury due to hindlimb ischemia/reperfusion injury only in the early phase of reperfusion.
- ItemAcesso aberto (Open Access)An experimental model for the study of collagen fibers in skeletal muscle(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-10-01) Calvi, Eliziane Nitz de Carvalho [UNIFESP]; Nahas, Fabio Xerfan [UNIFESP]; Barbosa, Marcus Vinicius [UNIFESP]; Calil, José Augusto; Ihara, Silvia Saiuli Miki [UNIFESP]; Silva, Marcelo de Souza; Franco, Marcello Fabiano de [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.
- ItemSomente MetadadadosMecanismo de ação dos alcalóides isolados do Geissospermum laeve (Vellozo) Bailon (Pau-Pereira) no músculo esquelético(Universidade Federal de São Paulo (UNIFESP), 1999) Tanae, Mirtes Midori [UNIFESP]; Lapa, Antonio José [UNIFESP]I. O Geissospermum laeve (Vellozo) Baillon conhecido no sudeste brasileiro como pau-pereira, é uma planta de casca amarga utilizada na medicina popular como febrífuga, tônica e hepatoprotetora. II. O Geissospermum laeve (Vellozo) Baillon é rico em alcalóides dos quais a geissospermina é a mais abundante. Também foram identificados a geissoschizina e a geissoschizolina (pereirina} como majoritários. III. A literatura descreve que os alcalóides do G. laeve têm ação curarizante. IV. Este trabalho objetivou isolar os alcalóides majoritários da casca do caule de G. laeve e estudar o mecanismo de ação daqueles com atividade na contração da musculatura esquelética. V. O material vegetal foi obtido de plantas do acervo do Jardim Botânico do Rio de Janeiro. VI. O pó das cascas secas foi extraído com etanol e por partiçoes sucessivas com clorofórmio / água em diferentes pH foi obtido um extrato rico em alcalóides. VII. Do extrato clorofórmico foram obtidos cristais identificados com análise química instrumental como geissospemina. VIII. Do extrato ácido foi obtida uma mistura de alcalóides denominada FAT. IX. A FAT foi purificada em CLAE fornecendo 4 frações ativas na preparação nervo frênico / músculo diafragma de camundongo. A fração mais ativa foi novamente purificada em CLAE e coluna de sílica gel fornecendo uma única fração ativa denominada F(4E)98-102. X. A geissospermina (3 a 100 mg/mL) bloqueou irreversivelmente as contrações do diafragma obtidas por estímulo direto e indireto. XI. Registros eletrofislológicos intracelulares mostraram que a geissospermina despolariza a membrana das fibras musculares, sem afetar especificamente a transmissão neuromuscular. XII Em miobolas de músculos de ratos recém nascidos, a geissospermina aumentou a condutância da membrana, impedindo o registro de correntes por "patch-clamp". Este efeito foi independente da presença de ACh e não foi bloqueado pela tetrodotoxina (300 nM). XIII. A F(4E)98-102 bloqueou a transmissão neuromuscular no diafragma de camundongo. O efeito foi revertido após lavagem mas não pela incubação de neostigmina. XIV. A F(4E)98-102 (1O a 30 mg/mL) diminuiu a amplitude dos potenciais em miniatura da placa terminal sem alterar a polaridade da membrana celular. A maior dose bloqueou a transmissão neuromuscular. XV. A incubação da F(4E)98-102 (1 a 10 mg/mL) em culturas de miobola diminuiu a freqüência de aberturas do canal iôníco associado ao receptor nicotínico e diminuiu ...(au).
- ItemAcesso aberto (Open Access)Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-04-01) Armstrong, Dinani Matoso Fialho de Oliveira; Armstrong, Anderson da Costa; Figueiredo, Regina Célia Bressan Queiroz; Florentino, Joao Eduardo; Saad, Paulo Fernandes [UNIFESP]; Fox-Talbot, Karen; Halushka, Marc Kenneth; Berkowitz, Dan E.; Taha, Murched Omar [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Sao Francisco Valley Federal University Department of Surgery; UNIVASF Department of Cardiology; Research Center Aggeu Magalhaes Department of Microbiology; Pernambuco Federal University; UNIVASF Department of Surgery; Johns Hopkins University Department of Pathology; Johns Hopkins University Department of Anesthesiology and Critical Care Medicine; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.