Navegando por Palavras-chave "Mycobacterium abscessus"
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- ItemSomente MetadadadosEnterobacterial repetitive intergenic consensus PCR is a useful tool for typing Mycobacterium chelonae and Mycobacterium abscessus isolates(Elsevier B.V., 2006-06-01) Sampaio, JLM; Viana-Niero, C.; De Freitas, D.; Hofling-Lima, A. L.; Leao, S. C.; Universidade Federal de São Paulo (UNIFESP); Inst Fleury Ensino & PesquisaOutbreaks of rapidly growing mycobacterium (RGM) infections are increasingly being reported worldwide. Information about genetic relatedness of isolates obtained during outbreaks can provide Opportunities for prompt intervention. Pulsed-field gel electrophoresis (PFGE) is expensive, time consuming, and labor intensive. Other than that, Mycobacterium abscessus isolates call suffer DNA degradation during electrophoresis. Polymerase chain reaction (PCR)-based methods are cheaper, faster, and easier to perform, but discriminatory power varies depending oil the primer used. Ill this study, we tested the competence of enterobacterial repetitive intergenic consensus (ERIC) PCR in comparison with PFGE to distinguish unrelated isolates (24 Mycobacterium chelonae and 24 M. abscessus) obtained front human and/or environmental samples and to group 56 isolates front 6 outbreaks confirmed epidemiologically, caused by M. chelonae and M abscessus after ophthalmologic refractive surgery and mesotherapy. Enterobacterial repetitive intergenic consensus PCR presented discriminatory power. calculated using Simpson's index of diversity, of 0.989 for M abscessus and 0.975 for M. chelonae and grouped outbreak isolates ill distinct groups showing epidemiologic concordance. Pulsed-field gel electrophoresis also grouped outbreak isolates and presented discriminatory power of 0.972 and 0.993 for M. abscessus and M. chelonae, respectively. DNA from 8 (22%) of 36 M. abscessus isolates analyzed showed degradation during electrophoresis. Compared with PFGE and epidemiologic information as the gold standard, ERIC PCR is a simple, high throughput, affordable, reproducible, and discriminatory molecular typing method for inference of genetic relatedness of RGMs of the M. chelonae-abscessus group. (c) 2006 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)Genomic epidemiology of a national outbreak of post-surgical Mycobacterium abscessus wound infections in Brazil(Microbiology Soc, 2017) Everall, Izzy; Nogueira, Christiane Lourenco [UNIFESP]; Bryant, Josephine M.; Sanchez-Buso, Leonor; Chimara, Erica; Duarte, Rafael da Silva; Ramos, Jesus Pais; Batista Lima, Karla Valeria; Lopes, Maria Luiza; Palaci, Moises; Kipnis, Andre; Monego, Fernanda; Andres Floto, R.; Parkhill, Julian; Leao, Sylvia Cardoso [UNIFESP]; Harris, Simon R.An epidemic of post-surgical wound infections, caused by a non-tuberculous mycobacterium, has been on-going in Brazil. It has been unclear whether one or multiple lineages are responsible and whether their wide geographical distribution across Brazil is due to spread from a single point source or is the result of human-mediated transmission. 188 isolates, collected from nine Brazilian states, were whole genome sequenced and analysed using phylogenetic and comparative genomic approaches. The isolates from Brazil formed a single clade, which was estimated to have emerged in 2003. We observed temporal and geographic structure within the lineage that enabled us to infer the movement of sub-lineages across Brazil. The genome size of the Brazilian lineage was reduced relative to most strains in the three subspecies of Mycobacterium abscessus and contained a novel plasmid, pMAB02, in addition to the previously described pMAB01 plasmid. One lineage, which emerged just prior to the initial outbreak, is responsible for the epidemic of post-surgical wound infections in Brazil. Phylogenetic analysis indicates that multiple transmission events led to its spread. The presence of a novel plasmid and the reduced genome size suggest that the lineage has undergone adaptation to the surgical niche.
- ItemSomente MetadadadosIn vitro activity of fluoroquinolones against Mycobacterium abscessus and Mycobacterium chelonae causing infectious keratitis after LASIK in Brazil(Lippincott Williams & Wilkins, 2005-08-01) Hofling-Lima, Ana Luisa [UNIFESP]; Freitas, Denise de [UNIFESP]; Sampaio, Jorge Luiz Mello [UNIFESP]; Leao, Sylvia Cardoso [UNIFESP]; Contarini, Patricia [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: To evaluate the in vitro activity of fluoroquinolones against Mycobacterium abscessus and Mycobacterium chelonae isolated from outbreaks of infectious keratitis in Brazil.Material and Methods: Micobacterial isolates were recovered from infectious keratitis cases related outbreaks that occurred in Brazil after LASIK for myopia. Two outbreaks occurred in Rio de Janeiro in 1998 and 1999, and 3 in Sao Paulo between 2000 and 2003. All laboratorial analysis, including molecular identification and antibiotic susceptibility testing with determination of the minimum inhibitory concentration (MIC) levels for ciprofloxacin, ofloxacin, gatifloxacin, and moxifloxacin, were performed at Universidade Federal de Sao Paulo in Brazil.Results: Fifteen samples were identified as M chelonae, and 3 were identified as M. abscessus. The outbreaks studied were designated SP-1 in 2000; SP-2 in 2000-2001; and SP-3 in 2003, R1 in 1988 and R2 in 1999. All but 1 of the M. chelonae were resistant to all fluoroquinolones with an MIC90 greater than 32 mu g/mL. The only susceptible isolate had MIC levels for ciprofloxacin, ofloxacin, gatifloxacin, and moxifloxacin of 0.38 mu g/mL, 0.032 mu g/mL, 0.047 mu g/mL, and 0.19 mu g/mL, respectively. MIC levels for all 3 M abscessus isolates tested were greater then 32 mu g/mL for all fluoroquinolones tested.Conclusions: Fluoroquinolone MICs for 17 M abscessus and M chelonae isolates recovered from infectious keratitis cases in Brazil indicate that they are not susceptible to these drugs in vitro. Further studies to investigate the in vivo effectiveness of fluoroquinolones against mycobacteria are required because in vitro tests do not support their use in the treatment of micobacterial keratitis in this particular geographic area.
- ItemAcesso aberto (Open Access)Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp massiliense CRM0019 in alveolar epithelial cells(Biomed Central Ltd, 2017) Ribeiro, Giovanni Monteiro [UNIFESP]; Matsumoto, Cristianne Kayoko [UNIFESP]; Real, Fernando [UNIFESP]; Teixeira, Daniela [UNIFESP]; Duarte, Rafael Silva; Mortara, Renato Arruda [UNIFESP]; Leao, Sylvia Cardoso [UNIFESP]; Carvalho-Wodarz, Cristiane de Souza [UNIFESP]Background: Outbreaks of infections caused by rapidly growing mycobacteria have been reported worldwide generally associated with medical procedures. Mycobacterium abscessus subsp. massiliense CRM0019 was obtained during an epidemic of postsurgical infections and was characterized by increased persistence in vivo. To better understand the successful survival strategies of this microorganism, we evaluated its infectivity and proliferation in macrophages (RAW and BMDM) and alveolar epithelial cells (A549). For that, we assessed the following parameters, for both M. abscessus CRM0019 as well as the reference strain M. abscessus ATCC 19977: internalization, intracellular survival for up 3 days, competence to subvert lysosome fusion and the intracellular survival after cell reinfection. Results: CRM0019 and ATCC 19977 strains showed the same internalization rate (approximately 30% after 6 h infection), in both A549 and RAW cells. However, colony forming units data showed that CRM0019 survived better in A549 cells than the ATCC 19977 strain. Phagosomal characteristics of CRM0019 showed the bacteria inside tight phagosomes in A549 cells, contrasting to the loosely phagosomal membrane in macrophages. This observation holds for the ATCC 19977 strain in both cell types. The competence to subvert lysosome fusion was assessed by acidification and acquisition of lysosomal protein. For M. abscessus strains the phagosomes were acidified in all cell lines
- ItemSomente MetadadadosMycobacterium abscessus, a taxonomic puzzle(Microbiology Soc, 2018) Tortoli, Enrico; Kohl, Tomas A.; Brown-Elliott, Barbara A.; Trovato, Alberto; Cardoso-Leao, Sylvia [UNIFESP]; Garcia, Maria Jesus; Vasireddy, Sruthi; Turenne, Christine Y.; Griffith, David E.; Philley, Julie V.; Niemann, Stefan; Wallace, Richard J., Jr.; Cirillo, Daniela M.
- ItemAcesso aberto (Open Access)Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil(Instituto Oswaldo Cruz, Ministério da Saúde, 2012-12-01) Matsumoto, Cristianne Kayoko [UNIFESP]; Chimara, Erica; Ramos, Jesus Pais; Campos, Carlos Eduardo Dias; Caldas, Paulo Cesar de Souza; Lima, Karla Valeria Batista; Lopes, Maria Luiza; Duarte, Rafael Silva; Leao, Sylvia Cardoso [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Instituto Adolfo Lutz; Escola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio Fraga; Instituto Evandro Chagas; Universidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de GóesA single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain.