Navegando por Palavras-chave "Mycosis"

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    Análise proteômica comparativa de isolados de p. brasiliensis e sua relação com a virulência fúngica
    (Universidade Federal de São Paulo (UNIFESP), 2018-06-07) Amaral, Cristiane Candida do [UNIFESP]; Camargo, Zoilo Pires de [UNIFESP]; http://lattes.cnpq.br/0632912481397728; http://lattes.cnpq.br/9021981707387327
    Paracoccidioidomycosis (PCM) is a systemic mycosis commonly found in Latin America that is caused by two distinct species of Paracoccidioides genus, Paracoccidioides brasiliensis complex (S1, PS2, PS3 e PS4) and Paracoccidioides lutzii. Its pathobiology has been recently explored by different approaches to clarify the mechanisms of host-pathogen interactions underpinning PCM. The diversity of clinical forms of this disease has been attributed to both host- and fungus-related factors. For better understanding of the molecular underpinnings of host-fungus interactions, we evaluated in vivo virulence of nine Paracoccidoides brasiliensis isolates and correlated it to protein expression profiles obtained by two-dimensional gel electrophoresis. Based on the recovery of viable fungi from mouse organs, the isolates were classified as those having low, moderate, or high virulence. Highly virulent isolates overexpressed proteins related to adhesion process and stress response, probably indicating important roles of those fungal proteins in regulating the colonization capacity, survival, and ability to escape host immune system reaction. Moreover, highly virulent isolates exhibited enhanced expression of glycolytic pathway enzymes concomitantly with repressed expression of succinyl-CoA ligase beta chain, a protein related to the tricarboxylic acid cycle. Our findings may point to the mechanisms used by highly virulent P. brasiliensis isolates to withstand host immune reactions and to adapt to transient iron availability as strategies to survive and overcome stress conditions inside the host.
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    Feline sporotrichosis due to Sporothrix brasiliensis: an emerging animal infection in São Paulo, Brazil
    (Biomed Central Ltd, 2014-11-19) Montenegro, Hildebrando; Rodrigues, Anderson Messias [UNIFESP]; Dias, Maria Adelaide Galvao; Silva, Elisabete Aparecida da; Bernardi, Fernanda; Camargo, Zoilo Pires de [UNIFESP]; Zoonosis Control Ctr São Paulo; Universidade Federal de São Paulo (UNIFESP)
    Background: Sporotrichosis is a mycotic infectious disease that is generally acquired by traumatic inoculation of contaminated materials especially from plant debris or through bites and scratches from diseased animals, such as domestic cats. It affects the skin, lymphatic system, and other organs in the warm-blooded host. Etiological agents are embedded in the plant-associated order Ophiostomatales. With essential differences between possible outbreak sources and ecological niche, host-environment interactions are classic determinants of risk factors for disease acquisition. Sporotrichosis outbreaks with zoonotic transmission, such as those that are ongoing in southern and southeastern Brazil, have highlighted the threat of cross-species pathogen transmission. Sporothrix brasiliensis has emerged as a human threat owing to the intimate contact pattern between diseased cats and humans in endemic areas.Results: We describe the recent emergence of feline sporotrichosis in the metropolitan region of São Paulo, Brazil, with an overwhelming occurrence of S. brasiliensis as the etiological agent. A phylogenetic and a haplotype approach were used to investigate the origin of this epidemic and the impact of feline transmission on genetic diversity. During the last 3-year period, 163 cases of feline sporotrichosis were reported in São Paulo with proven S. brasiliensis culture. the haplotype diversity of feline S. brasiliensis isolates revealed the expansion of a clonal population with low genetic diversity. Haplotype analysis confirmed that isolates from São Paulo shared the haplotype originated in the long-lasting outbreak of cat-transmitted sporotrichosis in Rio de Janeiro, which differed from the haplotype circulating in the Rio Grande do Sul epidemic.Conclusions: the fast spread of sporotrichosis in a short period of time highlights the potential for outbreaks and suggests that the mycosis may affect an urban population with a high concentration of susceptible felines. the feline sporotrichosis epidemic shows no signs of slowing, and this epidemiological pattern may require specific public health strategies to control future outbreaks.