Navegando por Palavras-chave "Network Analysis"

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    Effects of acute aerobic exercise on leukocyte inflammatory gene expression in systemic lupus erythematosus
    (Centro Federal Educacao Tecnologica Rio Grande Norte, 2016) Perandini, L. A.; Sales-de-Oliveira, Diego; Almeida, D. C. [UNIFESP]; Azevedo, Hatylas; Moreira-Filho, C. A.; Cenedeze, Marco Antonio [UNIFESP]; Benatti, F. B.; Lima, F. R.; Borba, Eduardo; Bonfa, E.; Sa-Pinto, A. L.; Roschel, H.; Camara, N. O.; Gualano, B.
    Systemic lupus erythematosus (SLE) is an autoimmune disease with a persistent systemic inflammation. Exercise-induced inflammatory response in SLE remains to be fully elucidated. The aim of this study was to assess the effects of acute exercise on leukocyte gene expression in active (SLEACTIVE) and inactive SLE (SLEINACTIVE) patients and healthy controls (HC). Methods: All subjects (n = 4 per group) performed a 30-min single bout of acute aerobic exercise (similar to 70% of VO2 peak) on a treadmill, and blood samples were collected for RNA extraction from circulating leukocyte at baseline, at the end of exercise, and after three hours of recovery. The expression of a panel of immune-related genes was evaluated by a quantitative PCR array assay. Moreover, network-based analyses were performed to interpret transcriptional changes occurring after the exercise challenge. Results: In all groups, a single bout of acute exercise led to the down-regulation of the gene expression of innate and adaptive immunity at the end of exercise (e.g., TLR3, IFNG, GATA3, FOXP3, STAT4) with a subsequent up-regulation occurring upon recovery. Exercise regulated the expression of inflammatory genes in the blood leukocytes of the SLE patients and HC, although the SLE groups exhibited fewer modulated genes and less densely connected networks (number of nodes: 29, 40 and 58
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    Identificação molecular de candidatos a biomarcadores/bioindicadores de impactos antrópicos através de micro-organismos aquáticos em região estuarina da Baixada Santista
    (Universidade Federal de São Paulo (UNIFESP), 2019-05-10) Garcia, Heitor [UNIFESP]; Gregoracci, Gustavo Bueno [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The estuarine region of Santos and Sao Vicente is amongst the most impacted areas of Brazil. In this context, it is possible to include ahigh number of ships bound to the greatest port in Latin America, an industrial district located in Cubatão near the estuary, and lack of sanitation. Despite its great importance, surface water quality is impacted by inefficient sewage collection and treatment and by industrial effluents released in the river path. To evaluate the antropic impacts we employed a culture independent methodology (shotgun metagenomics) to seek potential biomarker and/or bioindicator microorganisms which could determine environmental contamination of the water column. The methodology employed here optimized the analysed information by using incremented good quality data, which was added to the files and annotated in MG-Rast. We analysed physical-chemical parameters along the relative picoplankton abundances to correlate organisms and environment. Santos presented the smaller species richness and biodiversity, when confronted with Sao Vicente and control site Cananeia, probably due to larger income of chemical contamination, which likely was limiting factor to many micro-organisms. All sampling sites in this work have shown impacts related to sewage release in the marine environment or lack of treatment, including control site of Cananeia, which did not differ significatively from Sao Vicente, near palafitas. The current beach water balneability index seems ineffective, given the presence of many pathogenic microorganisms which impact human health and are not detected by this approach. Co-occurrence correlation networks are compromised for several physical-chemical parameters, and several pathogenic species are present. This problematic may be connected to the presence of metals and organic contaminants, and the proliferation of key microorganisms identified here may be necessary to make bioremediation possible and to lessen environmental impact. Marinomonas sp. MWYL1 and Marinobacter hydrocarbonoclasticus species could be sentinel organisms for HPA contamination and maybe biorremediation. Enterococcus faecalis and Staphylococcus aureus species seem to tolerate elevated NO2levels. Thalassiossira pseudonana maybe an import species to represent environmental water quality. Species from the Pseudomonas genus may be used as biomarkers of nitrogen contamination or bioremediation, being able to transform those compounds. This work makes it possible to observe the relation between microbial species and several physical-chemical parameters and identify targets which could enable healthier and more complete correlation networks, and maybe provide tools for future bioremediation of this environment.
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    Intragraft transcriptional profiling of renal transplant patients with tubular dysfunction reveals mechanisms underlying graft injury and recovery
    (Bentham Science Publ Ltd, 2016) Azevedo, Hatylas; Renesto, Paulo Guilherme [UNIFESP]; Chinen, Rogerio [UNIFESP]; Naka, Erika [UNIFESP]; Carvalho de Matos, Ana Cristina [UNIFESP]; Cenedeze, Marcos Antonio [UNIFESP]; Moreira-Filho, Carlos Alberto; Saraiva Camara, Niels Olsen [UNIFESP]; Pacheco-Silva, Alvaro [UNIFESP]
    Background: Proximal tubular dysfunction (PTD) is associated with a decreased long-term graft survival in renal transplant patients and can be detected by the elevation of urinary tubular proteins. This study investigated transcriptional changes in biopsies from renal transplant patients with PTD to disclose molecular mechanisms underlying graft injury and functional recovery. Methods: Thirty-three renal transplant patients with high urinary levels of retinol-binding protein, a biomarker of PTD, were enrolled in the study. The initial immunosuppressive scheme included azathioprine, cyclosporine, and steroids. After randomization, 18 patients (group 2) had their treatment modified by reducing cyclosporine dosage and substituting azathioprine for mycophenolate mofetil, while the other 15 patients (group 1) remained under the initial scheme. Patients were biopsied at enrollment and after 12 months of follow-up, and paired comparisons were performed between their intragraft gene expression profiles. The differential transcriptome profiles were analyzed by constructing gene co-expression networks and identifying enriched functions and central nodes in each network. Results: Only the alternative immunosuppressive scheme used in group 2 ameliorated renal function and tubular proteinuria after 12 months of follow-up. Intragraft molecular changes observed in group 2 were linked to autophagy, extracellular matrix, and adaptive immunity. Conversely, gene expression changes in group 1 were related to fibrosis, endocytosis, ubiquitination, and endoplasmic reticulum stress. Conclusion: These results suggest that molecular networks associated with the control of endocytosis, autophagy, protein overload, fibrosis, and adaptive immunity may be involved in improvement of graft function.